Nuclear Magnetic Resonance (NMR) Testing Service
- High-Field NMR Platform: Equipped with 400–600 MHz instruments, suitable for analyzing complex molecules at high resolution.
- Multi-Nucleus Detection: Supports ¹H, ¹³C, ³¹P, ¹⁹F, ¹⁵N, and more for versatile analytical applications.
- Experienced Technical Team: Our team has backgrounds in organic chemistry, pharmaceutical analysis, and structural biology, ensuring accurate data interpretation.
- Flexible Service Models: Offers one-time, batch, or long-term testing services tailored to project needs.
- Data Compatibility: Results can be used directly for publications, patents, and regulatory submissions.
- Comprehensive Reporting: Deliverables include raw spectra, processed files, experimental details, and preliminary structural evaluations for research and decision-making.
Nuclear Magnetic Resonance (NMR) is an advanced analytical technique based on the magnetic properties of atomic nuclei, widely used to study molecular structures and dynamic changes. Since its adoption in structural biology, pharmaceutical chemistry, and materials science, NMR has become an indispensable tool in modern research and industrial applications. Compared with other analytical methods, NMR is non-destructive, provides molecular-level structural information, and is applicable to various sample states including liquids and solids. It allows for the identification of bond types, spatial conformations, and substituent positions, making it widely used in organic synthesis validation, natural product identification, impurity profiling, and reaction monitoring.
Fan, S. et al. TCAS-I. 2022.
Figure 1. The Principle of NMR
Services at MtoZ Biolabs
MtoZ Biolabs, equipped with high-resolution NMR spectrometers ranging from 400 MHz to 600 MHz and supported by a professional analytical team, provides precise and reliable Nuclear Magnetic Resonance (NMR) Testing Services to academic institutions, universities, pharmaceutical companies, and industrial clients. Our comprehensive NMR solutions cover routine analysis of nuclei such as ¹H and ¹³C, as well as multi-nuclear detection including ³¹P, ¹⁹F, and ¹⁵N. According to clients’ research goals, our service includes:
1. 1D NMR analysis (e.g., ¹H NMR, ¹³C NMR)
2. 2D NMR analysis (e.g., COSY, HSQC, HMBC) for structural elucidation
3. Quantitative NMR (qNMR)
4. Dynamic NMR experiments (temperature/time dependent)
5. Purity and impurity profiling
6. Sample comparison and verification services
NMR is extensively used in the pharmaceutical industry for structural confirmation of APIs, impurity profiling, stability studies, chiral purity determination, and quantitative analysis of active ingredients in formulations. Its non-destructive nature and high resolution make it indispensable in early-stage drug screening, synthetic pathway verification, and quality control. MtoZ Biolabs provides standardized NMR testing support for new drug development and generic drug equivalence evaluation, assisting pharmaceutical companies in efficient R&D and regulatory submission. We also offer additional analytical services for chemical composition quantification and structure elucidation, including:
1. UV/Vis spectroscopy
2. Infrared (IR) spectroscopy
3. Raman spectroscopy
4. Mass spectrometry
5. Ion chromatography
6. High-performance liquid chromatography (HPLC)
7. Single crystal X-ray diffraction
Please feel free to contact us for more details about our services—we are here to help.
Analysis Workflow
1. Sample Preparation and Pre-Evaluation
Clients submit requests via email or our online system, along with sample details (compound type, solvent, suggested concentration). We provide clear submission guidelines and assist in selecting the appropriate testing strategy.
2. Test Plan Design
Based on the sample characteristics and client requirements, our specialists develop the testing plan including nucleus selection, scan parameters, and dimension setup.
3. Sample Testing and Data Acquisition
NMR spectra are collected under stable temperature conditions by experienced personnel, with optimized settings to ensure high signal-to-noise ratios and resolution.
4. Spectrum Processing and Preliminary Analysis
We process the raw data using professional software (e.g., TopSpin, MestReNova), including phase correction, integration, and chemical shift assignments.
5. Data Delivery and Technical Support
We provide full spectrum files, processed data, and PDF reports. If needed, we also offer structural confirmation or standardized technical reports.
Service Advantages
MtoZ Biolabs is committed to delivering high-quality, stable Nuclear Magnetic Resonance (NMR) Testing Service with the following core strengths:
Applications
Nuclear Magnetic Resonance (NMR) has broad application value across multiple fields and is suitable for researchers, pharmaceutical R&D personnel, and technical staff in new materials industries. The main application scenarios include:
1. Structural confirmation of organic small molecules: Used for structure identification of synthesized compounds, purity assessment, and differentiation of isomers.
2. Natural product research: Accurate identification of major components in plant or microbial metabolites.
3. Impurity analysis and quality control: Detection of low-level impurities in samples and precise quantification of components.
4. Drug development and synthesis pathway verification: Monitoring of reaction intermediates and support for mechanistic studies.
5. Materials science: Investigation of structural units and chemical environments in polymers and functional materials.
6. Structural analysis of biomacromolecules (under specific conditions): For example, studying the solution structure of proteins, which requires high concentration and specific labeling.
Case Study
1. NMR Can be Applied in Natural Product Research
Huang, Z. et al. Phytochem Anal. 2024.
Figure 2. The DOSY-COSY Spectrum of a Typical Herbal Preparation
FAQ
Q1. What is the recommended sample concentration for NMR?
A1: For ¹H NMR, 10–30 mg/mL is typical; for ¹³C NMR, 30–100 mg/mL. Requirements vary by sample and nucleus sensitivity.
Q2. What solvents are suitable for NMR testing?
A2: Common deuterated solvents include CDCl₃, DMSO-d₆, D₂O, and CD₃CN. Selection depends on sample solubility and target nuclei.
Q3. Can all elements be detected by NMR?
A3: No. Only nuclei with a non-zero nuclear spin (e.g., ¹H, ¹³C, ³¹P) can be detected. Nuclei like ¹²C and ¹⁶O have spin = 0 and are NMR-inactive.
Q4. How can labile protons (-OH, -NH) be better observed in ¹H NMR?
A4: Use dry solvents, dilute the sample, lower the temperature, increase relaxation delay (D1), and perform a D₂O exchange experiment.
Q5. Do you offer structure elucidation services?
A5: Yes. We provide basic spectrum interpretation and advanced structural analysis services upon request.
Q6. Is sample confidentiality guaranteed?
A6: Absolutely. All samples and results are handled under strict confidentiality and used exclusively for the client’s intended purpose.
MtoZ Biolabs combines a high-standard NMR platform with a professional scientific team to offer rigorous and streamlined NMR testing solutions. Whether you're conducting basic research, developing new compounds, or performing quality control, we provide efficient and dependable support. Contact us via our website or customer service to discuss a tailored NMR testing plan for your project.
How to order?
