Raman Spectroscopy Analytical Service
- Suitable for qualitative and quantitative characterization of micro areas or small-sized samples
- Commonly used for detecting internal defects in materials, as well as microscopic analysis in cells and tissues
- Dramatically enhances Raman signal intensity using metallic nanostructured substrates
- Suitable for trace-level detection (e.g., drug residues, environmental pollutants) and low-concentration component analysis
- Employs a confocal optical system for spectral acquisition at different depths or layers of the sample
- Capable of 3D Raman imaging, ideal for studying the spatial distribution of multilayer materials or biological tissues
- Obtains spectral information point by point across the sample surface or cross-section
- Visualizes the distribution, structure, and interactions of chemical components within the sample
- Investigation of biomacromolecular (proteins, polysaccharides, nucleic acids) conformations
- Cell and tissue section imaging, drug crystal form identification, and tablet surface analysis
- Identification and quantitative analysis of pollutants and microplastics in water, soil, or air
- Hazardous chemical detection, heavy metal speciation, and safety assessment
- Food quality and safety testing (e.g., component identification, adulteration analysis)
- Agricultural product quality evaluation and characterization
Raman Spectroscopy is a vibrational spectroscopic technique based on Raman scattering. When a laser beam irradiates the sample, most photons undergo Rayleigh scattering, while only a very small proportion of photons experience a change in energy to produce Raman scattering. By analyzing these Raman signals, one can obtain information on the vibrational or rotational modes of the sample’s molecules, thereby deducing its chemical bond structure, molecular conformation, and component distribution.
Muchaamba F, et al. Methods Protoc. 2024.
Figure 1. The Principal of Raman Spectroscopy
Raman spectroscopy offers a non-destructive analytical approach that requires no complex pretreatment or labeling, enabling direct measurement while preserving sample integrity. Its broad applicability spans solid, liquid, and gaseous samples across diverse fields, including materials science, life science, chemical engineering, environmental studies, and food science. To meet varied research needs, Raman spectroscopy supports multiple analytical modes such as Micro-Raman, Surface-Enhanced Raman Spectroscopy (SERS), Confocal Raman, and Raman Imaging. The following table summarizes the key advantages of Raman spectroscopy, common limitations encountered during its application, and corresponding solutions or complementary techniques.
Table 1. Advantages, Limitations, and Complementary Techniques of Raman Spectroscopy
By properly employing enhancement methods, adjusting testing parameters, or combining Raman with other characterization techniques (e.g., XRD, SEM, TEM, mass spectrometry), one can effectively mitigate limitations in sensitivity and interference, achieving more comprehensive and in-depth analysis of complex systems.
Services at MtoZ Biolabs
Building on a professional Raman spectroscopy platform and a team of experienced specialists, MtoZ Biolabs provides Raman Spectroscopy Analytical Service to meet scientific and industrial applications:
1. Micro-Raman
2. Surface-Enhanced Raman Spectroscopy (SERS)
3. Confocal Raman
4. Raman Imaging
Analysis Workflow
1. Requirement Consultation
Clarify sample type, research objectives, and application scope; determine the appropriate mode (Micro-Raman, SERS, Imaging, etc.).
2. Project Design
Select a suitable sample preparation method and Raman parameters (e.g., laser wavelength, grating resolution, exposure time) based on client requirements and sample properties.
3. Sample Testing
Carry out spectral acquisition under stable conditions; use SERS or confocal technology when needed for higher signal-to-noise ratios or depth-resolved information.
4. Data Processing and Analysis
Perform spectral pretreatment (baseline correction, noise filtering, etc.) and identify characteristic peaks for qualitative or quantitative analysis. If required, conduct 2D or 3D Raman imaging.
5. Report Preparation and Delivery
Provide detailed testing conditions, spectral results, component analysis, quantitative data, and expert interpretation of findings.
6. Follow-up Technical Support
Offer data re-analysis or multi-technique correlation as needed, and provide timely responses to subsequent inquiries.
Service Advantages
1. High Sensitivity and Resolution
State-of-the-art micro-Raman systems and surface-enhancement technology enable precise detection at microscopic or trace levels, fully covering the diverse needs of both research and industrial applications.
2. Multi-technology Integration
Offers Micro-Raman, SERS, Confocal Raman, and Raman Imaging; can incorporate additional techniques as appropriate, ensuring more comprehensive results.
3. Expert Data Interpretation
Our team has extensive experience in spectral interpretation and method development, ensuring accurate identification of Raman characteristic peaks and robust qualitative, quantitative, and structural data.
4. Customized, One-stop Service
MtoZ Biolabs manages the entire process—from experimental design and sample preparation to data analysis and report generation—delivering fully personalized, integrated solutions.
Applications
1. Life Sciences and Pharmaceuticals
2. Environmental Monitoring
3. Food and Agriculture
Case Study
1. Raman Spectroscopy for the Determination of Proteins Denaturation and Amino Acids Decomposition Temperature
This study employed Raman spectroscopy to investigate the thermal denaturation of three lyophilized proteins (BSA, lysozyme, and ovalbumin) and the thermal decomposition of three amino acids (L-glutamine, L-cysteine, and L-alanine). Raman spectra were recorded at predefined temperatures to determine the denaturation temperature (TD) and decomposition temperature (TM-dc). The results were highly consistent with data obtained from differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA), confirming the reliability and accuracy of Raman spectroscopy in thermal stability studies. Raman Spectroscopy Analytical Service supports monitoring thermally induced structural changes in proteins and small molecules, particularly in lyophilized samples. It enables the identification of conformational transitions and evaluation of critical transformation temperatures, providing valuable insights when integrated with thermal analysis data.
Ojeda-Galván, HJ. et al. Spectrochim Acta A Mol Biomol Spectrosc. 2023.
With its high sensitivity, non-destructive nature, and minimal interference from aqueous environments, Raman spectroscopy has become an indispensable characterization tool across numerous fields. MtoZ Biolabs is committed to providing high-quality, comprehensive Raman Spectroscopy Analytical Services for both scientific research and industrial enterprises, helping you rapidly obtain reliable molecular vibrational information and spatial distribution data. By flexibly utilizing various Raman detection modes and complementary analytical techniques, we deliver more complete and in-depth sample analysis. Should you require Raman spectroscopy or other multi-omics services, please feel free to contact us anytime. Let us join forces and drive innovation for the progress of science and industry!
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