High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening Service
High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening Service is a compound screening service based on high-throughput platforms, designed to systematically and rapidly discover and identify potential inhibitors of Sialyltransferases (STs) and fucosyltransferases (FUTs). By integrating advanced technologies such as fluorescence polarization (FP) assays and surface plasmon resonance (SPR), this service enables comprehensive evaluation of candidate molecules in terms of binding activity, inhibitory efficiency, and kinetic characteristics. It provides efficient and precise data support for drug discovery, mechanistic studies, and vaccine design.
STs and FUTs are important members of the glycosyltransferase family, responsible for catalyzing key glycosyl transfer reactions during glycan synthesis and modification. These enzymes play critical roles in regulating cell signaling, immune responses, cell adhesion, and pathogen infection. Their abnormal activities are closely associated with various diseases, including tumor metastasis, chronic inflammation, autoimmune disorders, and infectious diseases. Therefore, the development of ST and FUT inhibitors not only helps elucidate the roles of glycosylation in disease mechanisms but also provides novel targets for the development of anticancer, anti-infective, and immunotherapeutic drugs.
Rillahan C. et al. Nat Chem Biol. 2012.
Figure 1. Fluorinated Monosaccharide Analogs Act as Metabolic Glycosyltransferase Inhibitors.
Services at MtoZ Biolabs
Leveraging advanced high-throughput screening platforms and multidimensional analytical technologies, MtoZ Biolabs provides High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening Service to rapidly and systematically screen and identify potential transferase inhibitors, comprehensively evaluate their binding activity, inhibitory efficiency, and kinetic characteristics, thereby delivering robust data support and technical assurance for drug discovery, disease mechanism research, and immunotherapy development.
Analysis Workflow
The general workflow of the High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening Service is as follows:
1. Sample and Compound Library Preparation
Receive client-provided compound libraries or customized substrates, followed by necessary preprocessing and quality control.
2. High-Throughput Screening Experiments
Conduct preliminary screening using the FP platform, with validation and affinity measurement performed by SPR technology.
3. Data Acquisition and Processing
Collect experimental data and apply statistical and algorithmic analysis for candidate molecule screening and classification.
4. Report Delivery
Provide a detailed experimental and results report, including candidate inhibitor lists, kinetic parameters, and visualized charts.
Service Advantages
Advanced Analysis Platform: MtoZ Biolabs established an advanced High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
High Throughput and Efficiency: Enables rapid screening of hundreds to thousands of compounds, significantly shortening the discovery cycle for candidate molecules.
Customized Service: Flexibly adjusts screening strategies according to client objectives, supporting personalized drug development needs.
Real-Time Kinetic Monitoring: Provides real-time monitoring of enzyme–inhibitor interactions to accurately assess binding activity and affinity.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all High-Throughput Sialyl- and Fucosyltransferases Inhibitors Screening data, providing clients with a comprehensive data report.
Sample Submission Suggestions
Storage and Transportation
Compounds and enzyme samples should be stored at low temperatures (−20°C or below) and transported on dry ice to maintain stability.
It is recommended to contact the MtoZ Biolabs technical team prior to sample submission to obtain detailed and tailored guidelines for sample preparation and submission.
Applications
Disease Mechanism Studies
Elucidate the role of glycans in tumorigenesis, pathogen infection, and immune regulation.
Drug Discovery
Efficiently identify potential glycosyltransferase inhibitors and accelerate the development of glycosylation-targeted therapeutics.
Vaccine and Immunotherapy Development
Explore glycan modification–based antigen regulation strategies.
Glycosylation Modification Research
Systematically investigate the functions and regulatory mechanisms of glycosyltransferases in cell-surface glycan remodeling.
FAQ
Q1: What Are the Detection Sensitivity and Specificity of the Platform?
A1: The screening system at MtoZ Biolabs can detect inhibitor activity in the nanomolar to micromolar range, offering high sensitivity and high selectivity. With the inclusion of strict control groups and secondary validation, reliable results are ensured.
Q2: Can Clients Provide Their Own Compound Libraries?
A2: Yes, clients may submit self-constructed compound libraries (natural products or synthetic molecules), as long as they meet the basic solubility and purity requirements.
Q3: Is Data Visualization and Interpretation Provided?
A3: Yes, including heatmaps, scatter plots, binding kinetics curves, and more.
Related Services
High-Throughput Glycoengineered Cell Line Glycan Screening Service
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