Glycoproteomics Service

Glycoproteomics is an essential branch of proteomics that focuses on the systematic study of glycoproteins, which are proteins covalently linked with glycans at specific sites through post-translational modifications. Glycoproteins play crucial roles in cell recognition, signal transduction, immune regulation, and disease progression.

MtoZ Biolabs offers Glycoproteomics Service that integrates advanced mass spectrometry platforms with cutting-edge bioinformatics workflows to deliver comprehensive insights into glycoprotein structures and functions, empowering researchers in disease mechanism studies, biomarker discovery, and therapeutic development.

Services at MtoZ Biolabs

Our Glycoproteomics Service delivers end-to-end coverage, from sample preparation and glycoprotein enrichment to quantitative profiling, structural characterization, and functional annotation. Key offerings include:

Analysis Workflow

1. Sample Preparation

Protein extraction, denaturation, reduction, and enzymatic digestion to obtain peptides and glycopeptides suitable for downstream analysis.

2. Separation & Enrichment

Application of advanced chromatographic methods (HPLC, nanoLC) and affinity-based techniques to enhance resolution of glycoproteins and glycopeptides.

3. High-Resolution Mass Spectrometry

Qualitative and quantitative analysis using cutting-edge platforms (HPLC-MS/MS, MALDI-TOF MS).

4. Bioinformatics & Data Interpretation

Integration of glycoproteomics databases with advanced algorithms to identify glycosylation sites, characterize structures, and predict biological functions.

5. Comprehensive Reporting

Delivery of detailed results with biological insights to support both fundamental research and translational applications.

Figure 1. Glycoproteomics Service Workflow

Why Choose MtoZ Biolabs?

✔ Comprehensive Coverage: One-stop solutions spanning separation, enrichment, quantification, structural characterization, and functional annotation.

✔ High-resolution MS Platforms: Equipped with HPLC-MS/MS, MALDI-TOF, and advanced multi-stage fragmentation technologies for site-specific and structural precision.

✔ Low-abundance Detection: Specialized enrichment and sensitive detection strategies enable accurate profiling of low-abundance glycoproteins and complex isomers.

✔ Multi-omics Integration: Seamless integration with proteomics, metabolomics, and systems biology to reveal the broader role of glycosylation in biological networks.

Sample Submission Suggestions

We accept a wide range of biological and clinical samples, including cells, tissues, blood, serum, urine, saliva, and purified proteins. Clients only need to provide sample origin details. Pretreatment and analytical procedures will be carried out by our team. A detailed submission guide is available upon request.

Applications

· Biomarker Discovery: Identification of glycoproteins associated with cancer, autoimmune, and infectious diseases.

· Drug Development & Evaluation: Exploration of glycoproteins as therapeutic targets, supporting drug discovery and efficacy studies.

· Mechanistic Research: Investigation of glycoproteins in cell signaling, immune modulation, and pathogen-host interactions.

· Biopharmaceutical Quality Control: Monitoring glycoprotein drug consistency and ensuring structural fidelity during production.

FAQ

Q1: How does MtoZ Biolabs ensure high-precision identification of glycosylation sites?

We combine advanced glycopeptide enrichment with high-resolution mass spectrometry and multi-stage fragmentation (HCD, ETD, EThcD). This enables precise differentiation of glycopeptides and accurate site-specific localization of glycans.

Q2: Can your glycoproteomics service resolve glycan isomeric heterogeneity?

Yes. By integrating multidimensional chromatography (e.g., HILIC, PGC-LC) with MSn analysis, we obtain critical information on glycan linkages, branching, and conformations, allowing comprehensive resolution of isomeric structures.

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Site-specific Glycoprotein Identification Report

4. Data Analysis, Preprocessing, and Estimation

5. Optional Bioinformatics Insights

6. Raw Data Files

With cutting-edge instrumentation, robust workflows, and a team of experienced scientists, MtoZ Biolabs delivers high-quality, reproducible glycoproteomics data. Our glycoproteomics service not only reveals glycoprotein structures and functions but also drives biomarker discovery and therapeutic development.

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