Total Starch Content Analysis Service

Starch is one of the most abundant polysaccharides in nature and serves as an important source of energy for humans and animals. It is mainly composed of two glucose polymers, amylose and amylopectin. The ratio and structure of these two components vary depending on plant species and processing conditions, which determine the physicochemical properties and functional performance of starch. Whether in the food, feed, pharmaceutical, or biomaterial industries, accurately determining the total starch content of raw materials and products is critical for quality control, formulation optimization, and regulatory compliance.

 

Zhiguang, C. et al. NPJ Sci Food. 2025.

Figure 1. Left-hand Helix Amylose and Glycosidic Bond of Starch Molecules

 

Total starch content analysis quantitatively measures the total starch present in a sample, providing essential data on nutritional composition, formulation ratios, and stability for researchers and manufacturers. Accurate starch quantification not only ensures truthful product labeling but also influences process stability and batch consistency, forming the foundation for research and production across all stages.

 

Relying on advanced mass spectrometry, chromatography, and enzymatic analysis platforms, MtoZ Biolabs provides high-accuracy and high-reproducibility total starch content analysis service offering complete solutions from sample preparation to data interpretation. Based on internationally standardized methodologies and supported by a rigorous quality system, we ensure analytical precision, traceability, and global data comparability. Our services help clients across food, pharmaceutical, agricultural, and material industries achieve reliable quality evaluation and process optimization.

Services at MtoZ Biolabs

Accurate measurement of total starch content is a key step in ensuring product quality, safety, and compliance. With internationally recognized analytical methods, advanced instrumentation, and a professional technical team, MtoZ Biolabs provides scientific, systematic, and traceable total starch content analysis service that covers a wide range of sample types including grains, starch derivatives, plant tissues, feeds, excipients, and industrial additives. The analytical process strictly follows international standards such as AOAC, AACC, and ISO, ensuring the scientific validity and comparability of results.

 

Whether your focus is on food formulation development, pharmaceutical manufacturing, feed optimization, or biomaterial innovation, MtoZ Biolabs provides reliable analytical data and professional consultation. Contact us to learn more about our total starch content analysis service.

 

Analysis Workflow

1. Sample Preparation and Homogenization

Samples are milled, homogenized, and pre-dried to remove soluble sugars and moisture interference, ensuring representativeness and accuracy in testing.

 

2. Gelatinization and Enzymatic Hydrolysis

Using thermostable alpha-amylase and amyloglucosidase, starch is completely hydrolyzed into glucose monomers.

 

3. Glucose Quantification

Glucose concentration is determined by the glucose oxidase-peroxidase (GOPOD) method or hexokinase (Hexokinase/G6PDH) method through colorimetric or spectrophotometric analysis, ensuring high sensitivity and specificity.

 

4. Result Calculation and Correction

Total starch content is calculated based on the released glucose concentration, with corrections for sample dry weight, moisture, and blanks, yielding the final starch concentration.

 

Service Advantages

1. International Standard Compliance

MtoZ Biolabs strictly follows AOAC and AACC standardized analytical methods, using validated enzymatic hydrolysis and colorimetric detection systems. This ensures globally comparable data and regulatory acceptance. All processes are performed within a traceable quality framework, and results can be directly used for product registration, regulatory filing, or international collaboration.

 

2. Broad Sample Compatibility

Whether the sample is natural cereal, processed food, or modified starch, our total starch content analysis service enables efficient hydrolysis and precise quantification. By optimizing enzyme concentration, reaction temperature, and duration, we ensure stable and accurate results for starches with different structural characteristics.

 

3. Multi-Technology Integration

In addition to enzymatic assays, MtoZ Biolabs integrates analytical techniques such as HPLC, GC, and FTIR to investigate starch composition, molecular structure, and related saccharides. This multi-dimensional analytical approach provides clients with a more comprehensive carbohydrate characterization.

 

Applications

✔️ Food and Beverage Industry: For nutritional label verification, formulation optimization, and stability studies

✔️ Animal Feed Sector: To evaluate digestible carbohydrate content and energy utilization efficiency

✔️ Pharmaceutical Industry: To assess starch content in excipients and tablets ensuring formulation consistency

✔️ Agricultural Research: To study the effects of crop varieties or genetic modification on starch accumulation

✔️ Biomaterials and Bioplastics: To analyze starch-based composites and evaluate their component proportions and performance

 

Sample Submission Suggestions

1. Provide at least 5 grams of dry powdered sample for each test

2. Samples should be well-sealed to prevent moisture absorption or exposure to high temperatures

3. Indicate sample source, type, and intended purpose to ensure an optimized testing plan

4. Preliminary testing is recommended for complex or novel materials

 

Deliverables

1. Detailed explanation of analytical methods and workflow

2. Original data and absorbance or spectrum graphs

3. Calculated total starch content (both as-is and dry basis)

4. Validation results of standard materials and duplicate samples

5. Interpretation of results and notes on influencing factors

6. Deliverable files in PDF and Excel formats for documentation or publication