Resources

Proteomics Databases



Metabolomics Databases

• • What is the Protein Sequence?

  A protein sequence, defined as the arrangement of amino acids in a protein molecule, constitutes the primary structure of the protein, also known as its basic structure. This sequence not only determines the protein's three-dimensional conformation but also influences its biological function within the organism.   Protein Sequence—Types of Amino Acids Proteins are macromolecules made up of 20 standard amino acids linked by peptide bonds. These amino acids include glycine (Gly), alanine (Ala), valine (......

• • What are the Main Functions of Olink Proteomics?

  Olink proteomics is an advanced biotechnology that enables high-throughput, high-sensitivity, and high-specificity quantitative analysis of specific proteins in biological samples. This is achieved using the innovative Proximity Extension Assay (PEA) technology.   Core Technology and Advantages The central technology behind Olink proteomics is the PEA technique, which involves the specific recognition and binding of antibodies to target proteins, followed by the amplification of oligonucleotide se......

• • What is the Role of Protein Sequencing?

  Protein sequencing, as a key method for deciphering protein structure and function, plays an indispensable role in these processes. In 1954, Sanger successfully sequenced bovine insulin, confirming for the first time the role of amino acids in protein synthesis, thus marking the beginning of the protein sequencing field. In the early 1970s, Sanger developed DNA sequencing technology, laying the foundation for the modification of protein sequences. Proteins are critical macromolecular organic compound......

• • What are the Steps of Edman Degradation?

  Edman degradation involves a series of steps to determine the amino acid sequence of a protein or peptide, specifically targeting the N-terminal amino acid. These steps include: 1. Adsorption of the Sample The protein or peptide to be sequenced is adsorbed onto a solid surface, with glass fiber coated with polybutadiene (a cationic polymer) being a commonly used support material. This is the first of the steps of Edman degradation where the sample is prepared for further chemical reactions.   2. Coupl......

• • What is Protein Normalization Expression in Olink?

  The protein normalization expression in Olink is a critical aspect of the Proximity Extension Assay (PEA) platform developed by Olink. The principle of this method involves using two labeled oligonucleotide probes, which, upon binding to a target protein, bring the probes into close proximity, allowing for detection through PCR amplification. This technique is highly sensitive, capable of detecting low-abundance proteins while minimizing non-specific signal interference. The protein normalization expr......

• • What is the Edman Degradation Method?

  The Edman degradation method, developed by Pehr Edman in the 1950s, is a technique used for sequencing amino acids in a peptide or protein. This method sequentially removes one amino acid at a time from the N-terminal of the polypeptide chain. The cleaved amino acid is identified using high-performance liquid chromatography (HPLC) or similar techniques. Each cycle reveals one amino acid, and by repeating the process, the complete amino acid sequence can be determined.   Analysis Workflow The key steps......

• • Applications and Significance of Tandem Mass Spectrometry in Proteomics

  Tandem mass spectrometry in proteomics has rapidly advanced in recent years and has become an important analytical technique.Proteomics is one of the key areas in biological research that focuses on the study of proteins, which play crucial roles in biological systems. It is of great significance for understanding cellular functions and disease mechanisms.

• • Exploring the Application and Breakthroughs of Novel Electrophoresis Techniques in Protein Band Analysis

  Protein electrophoresis is a commonly used analysis method for separating and identifying proteins. To overcome the limitations of traditional electrophoresis technology and improve the analysis effect, researchers continue to explore and develop novel electrophoresis techniques in protein band analysis.

• • Protein Analysis Revealing Protein Functions and Interactions

  The techniques and methods for protein analysis include mass spectrometry analysis, electrophoresis analysis, spectroscopy analysis, and more. Mass spectrometry analysis provides information on protein composition, modifications, and quantification, which helps reveal the basis of protein function and interactions. Electrophoresis analysis can be used to separate and detect proteins, providing information on their size, charge, and purity.

• • Development of Protein Modification Quantification Methods Based on Proteomics Analysis

  Protein modification refers to the process of altering the biochemical characteristics and functions of proteins through covalent binding or non-covalent interactions.