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Proteomics Databases



Metabolomics Databases

• • How Do Peptide Sequences Differ from Protein Sequences in Structure and Function?

  Peptide sequences and protein sequences, while both composed of amino acids, differ significantly in their structural complexity, biological functions, and applications.   Structural Differences Peptide sequences are relatively small chains of amino acids, typically containing 2–50 residues linked by peptide bonds. Peptides may exist as linear chains or, less commonly, as cyclic structures. For instance, microcystins, cyclic peptides produced by cyanobacteria, are notable for their toxicity in aquatic......

• • What Distinguishes Sanger Sequencing from Edman Sequencing?

  Sanger sequencing and Edman sequencing differ significantly in their principles, applications, and limitations. A detailed comparison is provided below:   Target Molecules 1. Sanger Sequencing Designed for DNA sequencing, it identifies nucleotide sequences in nucleic acids.   2. Edman Sequencing Tailored for protein sequencing, it focuses on analyzing the N-terminal sequence of proteins.   Principles of Sequencing 1. Sanger Sequencing This method employs the dideoxy chain termination technique. DNA po......

• • What are the Standard Procedures for Performing Edman Sequencing?

  Edman sequencing, or Edman degradation, is a classical biochemical technique used to determine the N-terminal amino acid sequence of proteins. The standard procedures for performing Edman sequencing are described below:   Sample Preparation and Purification Before analysis, it is essential to assess the sample’s buffer composition, purity, and any chemical modifications. If the N-terminal amino group is blocked (e.g., by acetyl, formyl, or pyroglutamyl groups), the sample must undergo chemical or enzy......

• • How to Identify Target Proteins through mRNA Sequencing?

  How to Identify Target Proteins through mRNA Sequencing? The process involves the following steps: 1. Identify differential mRNAs through transcriptome analysis using high-throughput sequencing. 2. Validate the mRNAs using techniques such as qPCR. 3. Match the mRNAs to their corresponding proteins to identify the target proteins.   Detailed Process The identification of target proteins through mRNA sequencing involves a series of systematic steps that integrate experimental design, sequencing, data an......

• • What are the Methods of Protein Sequencing?

  The methods of protein sequencing are different techniques for revealing the amino acid sequence of proteins. Protein sequencing provides a direct means to determine the amino acid sequence of proteins, forming a cornerstone for studying protein structure and function. Sequencing facilitates the prediction of three-dimensional protein structures, offering insights into their biological functions and mechanisms of interaction.   Currently, the primary methods of protein sequencing include mass spectrom......

• • What are the Uses of Mass Spectrometry in Protein Analysis?

  Mass spectrometry in protein analysis is a powerful analytical tool that has become indispensable in numerous scientific fields, enabling researchers to examine the composition, structure, and functions of biomolecules with remarkable detail. Protein analysis, crucial for understanding biological processes, relies on mass spectrometry in protein analysis to reveal protein structures, functions, interactions, and regulatory mechanisms, which are foundational to advancements in disease diagnosis, drug d......

• • What is the Mass Spectrometry Technique for Proteins?

  Mass spectrometry technique for proteins is a sophisticated analytical technique used for the high-throughput identification and quantification of proteins. This approach involves ionizing protein or peptide samples, separating the resulting ions by their mass-to-charge ratios in a mass spectrometer, and then detecting them. By analyzing these ions, researchers can infer protein structure, functional characteristics, and interaction networks. Protein mass spectrometry has become invaluable in biomedic......

• • Why is Protein Sequencing Better than DNA Sequencing?

  Protein sequencing and DNA sequencing are two distinct molecular analysis techniques. DNA sequencing involves the analysis of deoxyribonucleic acid (DNA), the primary carrier of genetic information, which is located in the cell's nucleus. In contrast, protein sequencing focuses on proteins, which are the principal molecules performing diverse functions within the cell. Proteins are encoded by DNA and synthesized through RNA transcription and translation.   Common methods for DNA sequencing include San......

• • Mass Spectrometry-Based De Novo Protein Sequencing

  De novo protein sequencing refers to the process of determining the amino acid sequence of peptides using tandem mass spectrometry (MS/MS) without relying on pre-existing sequence databases.   The core principle of de novo protein sequencing is to calculate the mass of amino acid residues in the peptide backbone by measuring the mass difference between fragment ions. The mass-to-charge ratio (m/z) of these ions typically allows for the unique identification of the residue type, thereby determining the......

• • How is the Protein Sequence Obtained?

  Proteins are essential drivers of life processes, performing a wide array of functions, from catalyzing biochemical reactions to constructing cellular structures. Understanding protein structure and function is crucial in fields such as biology, medicine, and biotechnology. The protein sequence, which refers to the amino acid sequence, is a fundamental aspect of understanding both protein function and structure. This article outlines several methods for obtaining protein sequences.   Database Search W......