Resources

Proteomics Databases



Metabolomics Databases

• • Co-Immunoprecipitation Pull-Down Assay

  Co-immunoprecipitation pull-down assay is an established technique for analyzing protein-protein interactions. This method captures protein complexes interacting with a target protein through specific antibodies binding to it. Widely employed in molecular biology, the assay enables the verification of protein complex existence and composition. For instance, examining interactions between transcription factors and DNA-binding proteins enhances our understanding of gene expression regulation mechanisms.......

• • Collagen Analysis by HPLC

  Collagen analysis by HPLC is a widely utilized technique in proteomics research. The principle of collagen analysis by HPLC lies in the high resolution and sensitivity offered by liquid chromatography. This method involves preparing the sample through dissolution, filtration, and injection into the HPLC system. The sample is then separated within the chromatographic column, where it interacts with the stationary and mobile phases. Each component is progressively eluted based on its partitioning behavi......

• • Co-Immunoprecipitation and Pull-Down Assays

  Co-immunoprecipitation and pull-down assays are pivotal techniques for investigating protein-protein interactions. These methods are fundamental in modern molecular biology and proteomics research, facilitating the exploration of intricate cellular protein networks. Co-IP is predominantly used to verify established protein interactions or to detect unknown interacting partners within the same biological pathway. Pull down assays employ a tagged "bait" protein to isolate "prey" proteins that bind to it......

• • Co-Immunoprecipitation Protein-Protein Interaction

  Co-immunoprecipitation protein-protein interaction is a widely used experimental technique in biochemical and molecular biology research. It involves capturing the target protein and its interacting partners using specific antibodies, enabling the identification and analysis of protein complexes. The typical Co-IP process includes three main steps: first, selecting an antibody that specifically binds to the target protein; second, using the antibody to capture the target protein, followed by precipita......

• • Amino Acid Analysis of Proteins

  Amino acid analysis of proteins is a technique for the qualitative and quantitative detection of amino acid composition and modifications within proteins. Amino acids are the basic building blocks of proteins, and their specific sequence determines the protein's structure and function. By performing amino acid analysis of proteins, researchers can uncover insights into protein structure, function, and changes associated with diseases or metabolic disorders. This technology is widely used in proteomics......

• • Amino Acid Sequence Analysis

  Amino acid sequence analysis is a technique used to determine the order of amino acids in protein molecules. The structure and function of proteins are directly determined by their amino acid sequence, with amino acids linked by peptide bonds in a specific order to form protein molecules with distinct three-dimensional structures. The key advantages of amino acid sequence analysis include high sensitivity, high resolution, and high throughput. Mass spectrometry, in particular, is well-suited for analy......

• • Acetylation Mass Spectrometry

  Acetylation mass spectrometry is a specialized mass spectrometry technique for analyzing the post-translational modifications (PTM) of proteins, focusing specifically on the detection and identification of acetylation sites and performing both qualitative and quantitative analyses. Acetylation is a crucial PTM, prevalent in both eukaryotic and prokaryotic proteins, where it regulates key biological processes including gene expression, metabolic pathways, the cell cycle, and signal transduction. The de......

• • Amino Acid Analysis Mass Spectrometry

  Amino acid analysis mass spectrometry is an efficient technique for separating, quantifying, and identifying amino acids. Amino acids are the fundamental building blocks of life, involved not only in protein synthesis but also in cellular signal transduction and energy metabolism. Using high-resolution mass spectrometry, this technique can accurately measure the types, concentrations, and distributions of amino acids in a sample, revealing complex metabolic networks and physiological states in organis......

• • ABPP Assay

  The ABPP Assay is a powerful chemical proteomics technique used for the specific labeling, separation, and identification of active enzymes or proteins in complex biological systems. Unlike traditional proteomics methods, the ABPP assay emphasizes not only protein expression levels but also the dynamic detection of protein activity. This technique utilizes small-molecule probes that covalently bind to the active sites of target proteins, enabling highly specific identification of their functional stat......

• • Absolute Quantification Mass Spectrometry

  Absolute quantification mass spectrometry is a method used for the precise measurement of the absolute levels of specific proteins in biological samples, leveraging mass spectrometry technology. Unlike traditional relative quantification techniques such as iTRAQ, TMT, or label-free quantification, this approach provides precise quantification of target proteins by employing standards or isotopically labeled internal standards. This method circumvents potential errors introduced by sample variability, ......