Resources
Proteomics Databases
Metabolomics Databases
-
Tandem mass spectrometers enable precise qualitative and quantitative analysis of complex mixtures by connecting two or more mass analyzers in series, leveraging their ability to separate, detect, and analyze ions. These instruments are especially effective in analyzing complex, low-abundance biomolecules such as proteins, metabolites, and drug molecules. Their application in proteomics is particularly significant. By conducting precise mass spectrometry analysis of peptides, scientists can decode the......
-
Tandem mass spectrometry is a highly sensitive analytical technique for investigating the components of complex mixtures, with significant applications in proteomics research. This method divides the mass spectrometry process into sequential steps, enabling the identification and quantification of specific molecules in complex samples. The basic principle involves selecting a target ion in the first mass spectrometer and fragmenting it using collision-induced dissociation (CID) or other activation met......
-
The peptide mapping workflow encompasses enzymatic digestion, chromatographic separation, and mass spectrometry analysis of target proteins or antibodies to construct detailed peptide maps. These maps provide insights into molecular structures, post-translational modifications, and sequence variants. As a cornerstone for protein structure characterization and quality control, this workflow plays an integral role in drug development, biomarker discovery, and process optimization. Basic Workflow The p......
-
Peptide Mass Mapping is a core data form in proteomics and bioanalytical research. It is generated by detecting the mass-to-charge ratio (m/z) of peptides using a mass spectrometer, producing specific spectral signals to deduce peptide sequence information and post-translational modifications. It serves as a crucial tool for understanding protein molecular structure, function, and interactions, holding irreplaceable value in biopharmaceutical development, disease mechanism research, and fundamental li......
-
• Unlocking Hidden Secrets: Comprehensive Strategies for Unknown Protein Analysis
The field of unknown protein analysis relies on systematic approaches to uncover the structure, function, and biological roles of these enigmatic molecules. Advancements in Genomics and Transcriptomics The foundation of unknown protein analysis lies in leveraging high-throughput sequencing to extract genomic and transcriptomic data. These datasets enable the identification of novel genes and transcripts encoding unknown proteins. For instance, whole-genome sequencing may reveal genes with no known f......
-
• Identification of Unknown Proteins in Mixed Protein Samples
The identification of unknown proteins in mixed protein samples is a cornerstone technique in biological research, enabling the characterization of individual proteins within complex mixtures. This method is essential for applications in drug development, biomarker discovery, and the investigation of disease pathways. As a fundamental component of proteomics, the identification of unknown proteins in mixed protein samples continues to drive advancements in understanding biological systems. Identific......
-
• Determination of Unknown Proteins
The determination of unknown proteins encompasses several systematic approaches: 1. Genomic Strategies Gene sequencing provides essential data for identifying unknown proteins. Bioinformatics tools analyze genomic sequences to predict potential protein-coding regions, contributing to the determination of unknown proteins. 2. Proteomics Applications Proteomics facilitates comprehensive analysis of cellular protein types and interactions, offering critical insights for the determination of unknown pro......
-
• Unveiling the Mysteries of Unknown Proteins: From Detection Techniques to Data Interpretation
The determination of unknown proteins requires a multidisciplinary approach involving advanced technologies for detection, analysis, and validation: Detection Techniques 1. Mass Spectrometry This sensitive analytical method enables precise identification of unknown proteins by determining molecular weights, amino acid sequences, and post-translational modifications. 2. Genomics and Transcriptomics Methods such as RNA sequencing and gene expression profiling provide evidence for unknown proteins by......
-
• Identification of Unknown Proteins
Identification of unknown proteins is a critical task in life sciences, as proteins are fundamental to life, driving essential cellular functions and providing structural integrity. Despite their importance, many proteins remain uncharacterized. The identification of these unknown proteins is vital for uncovering the mechanisms of biological systems, advancing therapeutic development, and pinpointing novel drug targets. The complexity and diversity of life can be better understood through the identi......
-
• How to Identify Unknown Proteins
The process to identify unknown proteins involves a series of carefully coordinated steps: Protein Extraction and Purification Proteins are extracted from cell or tissue samples and purified using techniques like centrifugation, chromatography, or electrophoresis to isolate the target protein for subsequent analysis. Protein Separation Gel electrophoresis methods, such as SDS-PAGE, are commonly applied to separate proteins based on molecular size or charge. Protein Identification 1. Mass Spectro......
How to order?
