Resources

Proteomics Databases



Metabolomics Databases

• • Circular Dichroism (CD) Spectroscopy

  Circular dichroism (CD) spectroscopy is a fundamental spectroscopic tool widely employed for molecular structural analysis, especially the study of biomacromolecule conformations. The method utilizes the differential absorption of circularly polarized light to provide structural insights, making it particularly useful in characterizing the secondary structures of proteins and nucleic acids. Understanding such structural details is critical since the biological activity of these macromolecules depends ......

• • Advancing Biomolecular Studies with Circular Dichroism CD Spectroscopy

  Circular dichroism CD spectroscopy is a pivotal technique in molecular structure analysis, particularly for exploring the secondary structures of biomacromolecules. This spectroscopic method leverages the differential absorption of circularly polarized light to derive detailed structural information. Its application is especially prominent in analyzing proteins and nucleic acids. By measuring the spectral characteristics in the far-UV and near-UV regions, CD spectroscopy reveals critical data on α-hel......

• • Advancing Drug Discovery with Activity-Based Protein Profiling

  Activity-based protein profiling integrates proteomics technologies with bioactivity studies to investigate protein functionality and biological responses under disease or drug influence by detecting dynamic activity changes. Proteins are pivotal to cellular processes, with their functions often manifesting through activity, such as enzymatic catalysis, protein-protein interactions, and signal transduction. This profiling method leverages activity monitoring to elucidate the roles of proteins in compl......

• • Unveiling Functional Proteomics with Activity-Based Protein Profiling

  Activity-based protein profiling (ABPP) is a state-of-the-art technology that leverages small molecule probes to selectively label and analyze active proteins in biological systems. Through the integration of mass spectrometry, ABPP enables high-throughput functional studies of proteins, emphasizing their activity states rather than their abundance. This unique feature allows ABPP to uncover dynamic protein function and has become an indispensable tool in studying enzyme activity, identifying drug tar......

• • Co IP Assay

  The Co IP assay is a cornerstone technique in studying protein-protein interactions, extensively applied across basic biology, drug discovery, and disease mechanism research. By leveraging this approach, researchers gain insights into intracellular protein functions, regulatory mechanisms of signaling pathways, and roles in pathological conditions. This technique capitalizes on the specific binding properties of antibodies to isolate target proteins and their associated complexes. Combining immunologi......

• • Immunoprecipitation and Coimmunoprecipitation

  Immunoprecipitation and coimmunoprecipitation are fundamental techniques in proteomics, widely applied to investigate protein structures, functions, and dynamic changes in cellular environments. These methods utilize the specific binding between antibodies and target proteins. Immunoprecipitation (IP) isolates a target protein from complex biological samples via antibodies, enabling analysis of protein purity and interaction with other proteins through coimmunoprecipitation. The Co-IP technique extend......

• • Co Immunoprecipitation Assay

  The co immunoprecipitation assay serves as a cornerstone technique in protein interaction studies, employing specific antibodies to isolate protein complexes from complex biological samples. This method capitalizes on the selective binding of antibodies to target proteins, facilitating the precipitation of associated protein complexes and elucidating intracellular protein interaction networks. Its versatility extends to identifying protein partners, unraveling biological mechanisms, understanding dise......

• • ABPP Activity Based Protein Profiling

  ABPP activity based protein profiling is a function-oriented proteomics approach that prioritizes the activity and functional states of proteins over traditional metrics such as expression levels or sequence characteristics. This technique has emerged as a pivotal method for understanding protein functionality, identifying active proteins, and elucidating protein-small molecule interactions. By employing specific reactive probes, ABPP selectively labels active proteins, enabling their capture, enrichm......

• • HPLC Tandem Mass Spectrometry

  High-performance liquid chromatography tandem mass spectrometry (HPLC tandem mass spectrometry, HPLC-MS/MS) is a critical analytical technique widely applied in modern analytical chemistry, particularly for analyzing components in complex biological samples. By combining the separation power of high-performance liquid chromatography (HPLC) with the analytical capabilities of mass spectrometry (MS), HPLC-MS/MS enables the efficient separation, identification, and quantification of samples within a sing......

• • Quantify Protein

  Protein quantification analysis is one of the core tasks in proteomics research, aiming to precisely quantify protein levels by measuring the relative or absolute abundance of proteins in a sample. This process not only unveils the dynamic changes in cellular protein levels but also provides valuable insights into various biological conditions, such as organism health, disease states, and drug responses. Proteins are fundamental functional molecules in living organisms and participate in almost every ......