Resources

Proteomics Databases



Metabolomics Databases

• • Single-Cell Spatial Transcriptomics

  Single-cell spatial transcriptomics is an innovative technology that integrates single-cell transcriptomics with spatial information, allowing for the precise measurement of gene expression in individual cells within tissue sections. By achieving single-cell resolution, this method has transformed biological and medical research. Unlike traditional transcriptomics, which averages gene expression data across large populations of cells and overlooks cellular heterogeneity, single-cell spatial transcript......

• • Cryo-EM

  Cryo-electron microscopy (Cryo-EM) is a transformative imaging technique for investigating the three-dimensional structures of biological macromolecules. By operating at cryogenic temperatures, Cryo-EM allows scientists to capture detailed images of proteins, viruses, and other complex biomolecules in their native states. The method involves rapidly freezing samples to vitrify them in amorphous ice at liquid nitrogen temperatures, preserving their natural structure and preventing distortions caused by......

• • Isolation, Purification, and Characterization of Enzymes

  The isolation, purification, and characterization of enzymes are critical processes in modern biochemistry and biotechnology. These methods enable researchers to extract specific enzymes from complex biological samples and thoroughly analyze their properties. Enzymes, as essential biological catalysts, drive nearly all biochemical reactions, playing pivotal roles in metabolism, signal transduction, cellular communication, and gene expression. Studying the structure, function, and roles of enzymes in b......

• • Protein Sequencing Using Mass Spectrometry

  Protein sequencing using mass spectrometry is a cutting-edge technology for determining the amino acid sequence of proteins with precision. By leveraging mass spectrometry, researchers can obtain detailed information on the amino acid composition and sequence of proteins. The core principle involves ionizing protein samples, fragmenting them into smaller peptide chains, and measuring their mass-to-charge ratio (m/z) to deduce the amino acid sequence. This approach is central to proteomics, offering va......

• • Edman Degradation for Protein Sequencing

  Edman degradation for protein sequencing is a fundamental technique in life science research, essential for elucidating protein structures and functions. This method, introduced decades ago, has become a cornerstone in protein chemistry. It sequentially cleaves and labels the N-terminal amino acids of a polypeptide chain, enabling precise determination of a protein’s primary structure. Its unparalleled accuracy and reliability have established it as a widely used approach in protein science, drug disc......

• • Enzyme Isolation, Purification, and Characterization

  Enzyme isolation, purification, and characterization are integral to modern biochemistry and biotechnology. Enzymes, as biological catalysts, are central to a wide range of biological processes and applications. These steps facilitate the production of highly pure and active enzymes, enabling their effective use in research, industrial production, and medical diagnostics.   Enzyme isolation involves extracting enzymes from biological materials, while purification removes impurities to achieve the nece......

• • Characterization of Protein and Nucleic Acid

  The characterization of protein and nucleic acid is a critical technique for elucidating the complex structures and functions of biomolecules. This process encompasses the detailed examination of their structures, sequences, interactions, and dynamic behaviors, providing insights into their roles and mechanisms within biological systems. Proteins serve as the functional machinery of life, driving essential cellular activities such as catalysis, growth, and signaling. Nucleic acids, in contrast, act as......

• • Cryo-EM of Membrane Proteins

  Cryo-EM of membrane proteins is an advanced technique leveraging cryogenic electron microscopy to resolve the high-resolution three-dimensional structures of membrane proteins. Membrane proteins, embedded in or associated with biological membranes, play pivotal roles in processes such as signal transduction, material transport, and energy conversion. However, their complex structures and low stability pose significant challenges for traditional methods like X-ray crystallography and nuclear magnetic r......

• • Single-Molecule Protein Sequencing

  Single-Molecule Protein Sequencing is designed to precisely determine the amino acid sequence of individual protein molecules. Unlike traditional protein sequencing methods, which require analyzing large quantities of identical protein molecules, this technique directly reads sequence information at the single-molecule level. Its key advantage lies in exceptional sensitivity and specificity, allowing detection of trace amounts of proteins, including those present in very low abundance. This capability......

• • Protein Microarray Analysis

  Protein Microarray Analysis is a high-throughput and efficient biotechnological approach for investigating molecular interactions, including protein-protein, protein-drug, and protein-nucleic acid interactions. This method relies on immobilizing a diverse array of protein molecules onto a solid substrate in an orderly manner. By introducing probe molecules-such as fluorescently labeled antibodies or drugs-specific binding events can be detected, allowing researchers to measure protein expression level......