Resources
Proteomics Databases
Metabolomics Databases
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Metabolomics and proteomics provide complementary insights into biological activities at the molecular level, with metabolomics focusing on small molecule metabolites and proteomics on proteins. These two fields are fundamental to modern biological research. Metabolomics studies metabolites, which are intermediate or final products of biochemical reactions. The dynamic fluctuations in metabolite levels reflect cellular metabolic states and responses to environmental changes. By analyzing the types, co......
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SWATH mass spectrometry is an advanced data-independent acquisition technique that has attracted considerable attention in proteomics research in recent years. This technology addresses the challenge of inadequate coverage of low-abundance proteins in traditional data-dependent acquisition approaches by fragmenting all ions within a predefined mass range. The introduction of this technique has significantly enhanced the coverage and reproducibility of quantitative proteomics data, as well as improved ......
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• Molecular and Cellular Proteomics
Molecular and Cellular Proteomics is a scientific discipline that investigates the composition, structure, function, and dynamic changes of proteins within living systems. Its goal is to uncover the mechanisms through which proteins function at the molecular and cellular levels. As a cutting-edge field in life sciences, it integrates mass spectrometry, advanced separation techniques, and bioinformatics, providing powerful tools for probing complex biological systems. By analyzing the expression profil......
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Bottom up proteomics is a widely used strategy for qualitative and quantitative analysis of proteins in proteomics research. Unlike top-down proteomics, the core principle of bottom up proteomics involves digesting the entire protein sample into smaller peptides prior to analysis. These peptides are then analyzed in detail by mass spectrometry (MS). This process allows for the precise investigation of protein structure, function, and dynamic changes, facilitating the efficient analysis of thousands of......
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Single-cell proteomics (SCP) is a cutting-edge analytical technique that deciphers protein composition and function at the resolution of individual cells. By uncovering cellular-level details, single-cell proteomics reveals intercellular heterogeneity and addresses limitations of traditional bulk proteomics, which provide only average or aggregate protein expression data. In plant research, single-cell proteomics circumvents challenges posed by rigid cell walls that hinder traditional methods from acc......
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• Quantitative Mass Spectrometry
Quantitative mass spectrometry is primarily employed to measure the concentrations of proteins or other molecular components in biological samples, providing insights into their roles in specific biological processes or disease states. This technique utilizes a mass spectrometer to analyze the mass, abundance, and other characteristics of target molecules with high sensitivity. By integrating standardized quantitative methods, it enables precise measurement of molecular information in complex biologic......
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CD spectroscopy of proteins is a versatile and widely used technique for investigating the conformational and dynamic properties of biological macromolecules. By measuring the differential absorption of left- and right-handed circularly polarized light, it provides critical insights into molecular conformation. Since the secondary structure of proteins directly influences circular dichroism spectra, this method finds broad applications in monitoring conformational changes, studying stability, analyzin......
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• Circular Dichroism for Proteins
Circular dichroism for proteins is a spectroscopic technique widely used to analyze the secondary structures of proteins, their conformational changes, and interactions with ligands. This method leverages the optical phenomenon of circular dichroism, which arises from the differential absorption of left- and right-handed circularly polarized light by chiral molecules. By recording CD spectra across various wavelengths, researchers can deduce the secondary structure composition of proteins, including α......
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• Circular Dichroism CD Spectra
Circular dichroism CD spectra is an essential spectroscopic technique widely used to investigate molecular structures, leveraging the differential absorption of circularly polarized light to provide insights into molecular chirality and configuration. This technique plays a crucial role in studying biomacromolecules, particularly proteins and nucleic acids, under near-physiological and non-destructive conditions. Circular dichroism CD spectra is invaluable in protein research for determining secondary......
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CD spectra of protein is a pivotal spectroscopic technique for exploring the structural properties of proteins. By measuring the differential absorption of left- and right-handed circularly polarized light, CD spectra offer detailed insights into the secondary structures of proteins. These secondary structures, including α-helices, β-sheets, and random coils, play crucial roles in determining protein function. Analyzing CD spectra of protein enables researchers to unravel intricate details about p......
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