Resources
Proteomics Databases
Metabolomics Databases
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LC-MALDI-TOF integrates liquid chromatography (LC) with MALDI-TOF mass spectrometry, offering a powerful analytical platform widely used in proteomics and bioanalysis. LC enables the separation of complex biological samples, ensuring the isolation of individual components at defined time intervals. The separated components are mixed with a matrix-a compound that absorbs laser photons-preparing them for MALDI-based mass spectrometry. During analysis, laser pulses excite the matrix, resulting in ionizat......
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Untargeted proteomics is a technique that systematically investigates and analyzes all detectable proteins and their modifications in biological samples without relying on prior assumptions about specific targets. Unlike targeted proteomics, which focuses on predefined protein sets, untargeted proteomics employs high-throughput mass spectrometry to capture global protein expression profiles, modification patterns, and their changes under varying physiological and pathological conditions. This comprehe......
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Neoantigen identification involves identifying and validating novel antigen molecules produced by specific tumors using bioinformatics tools and experimental methods. These neoantigens are derived from tumor-specific mutations that differ significantly from normal cell antigens, enabling them to be recognized by the immune system and initiate immune responses. Neoantigens arise due to genetic mutations accumulated during the proliferation and division of cancer cells, resulting in the production of ab......
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iTRAQ analysis is a high-throughput quantitative method widely used in proteomics research. By incorporating isotopic labels at peptide termini, this technique allows for the simultaneous relative and absolute quantification of proteins across multiple biological samples. The core principle of iTRAQ lies in its use of isotopic tags, which ensure identical mass signals for labeled samples in the mass spectrum. During collision-induced dissociation (CID), these tags release reporter ions with distinct m......
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GC-MS quantitative analysis combines the capabilities of gas chromatography and mass spectrometry to provide a powerful tool for analyzing complex samples. Gas chromatography separates volatile compounds with high efficiency, while mass spectrometry detects and identifies their masses and structures with exceptional sensitivity and specificity. This combination enables GC-MS to deliver both qualitative and quantitative data in a single analysis, making it invaluable in modern research and industrial a......
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PTM mass spectrometry is a powerful analytical technique for identifying and characterizing post-translational modifications (PTMs) in proteins. PTMs, including phosphorylation, acetylation, ubiquitination, and methylation, are chemical modifications that regulate critical aspects of protein function such as activity, stability, localization, and molecular interactions. By providing detailed insights into modification types, sites, and abundances, PTM mass spectrometry plays a pivotal role in understa......
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String protein analysis leverages the extensive bioinformatics resources provided by the STRING database to investigate and understand protein-protein interactions within organisms. The STRING database is an online platform that integrates and provides information on experimentally validated and computationally predicted protein-protein interactions. Through this resource, researchers can generate protein interaction networks, facilitating a deeper understanding of the functions and relationships of p......
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• X-ray Crystallography Protein Structure Determination
X-Ray Crystallography protein structure determination is a core method in structural biology, enabling the elucidation of protein three-dimensional (3D) structures with atomic-level resolution. Protein function is intricately linked to its 3D structure, and this technique provides unparalleled accuracy for visualizing protein architectures. The process involves exposing protein crystals to X-rays, capturing diffraction patterns, and using these data to reconstruct the protein’s 3D structure. This me......
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Shotgun Mass Spectrometry is a powerful technique for the identification and quantification of proteins in complex biological samples. Its fundamental principle involves measuring the mass-to-charge ratio (m/z) of molecules within a sample using a mass spectrometer, which allows for the precise analysis of protein mass and structural characterization. A key advantage of this method is its high sensitivity and resolution, which enable the identification of thousands of proteins from numerous samples in......
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• Determination of Amino Acid Composition
The determination of amino acid composition is an essential analytical technique in protein chemistry and biology, used to quantify the proportion and content of amino acids in proteins or peptides. This method involves hydrolyzing proteins into individual amino acids, followed by precise quantification using advanced separation and detection techniques. It provides critical data for understanding protein structure, function, and metabolism. The determination of amino acid composition holds particular......
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