Resources

Proteomics Databases



Metabolomics Databases

• • Mass Spectrometry in Protein Analysis

  Mass spectrometry in protein analysis has become indispensable for understanding complex cellular processes at the molecular level. Mass spectrometry is a pivotal technique in protein analysis, providing essential information on the mass, structure, composition, and quantification of proteins. Through mass spectrometry analysis, researchers can identify unknown proteins, characterize protein complexes, detect post-translational modifications, quantify protein levels, and investigate protein dynamics. ......

• • Proteomics Research Using Mass Spectrometry

  Proteomics research using mass spectrometry (MS) has become indispensable for identifying and quantifying cellular proteins, offering insights into their functions and interactions. As a cornerstone of biomedical innovation, mass spectrometry has propelled advancements in understanding disease mechanisms, biomarker discovery, and drug development.   Applications of Mass Spectrometry in Proteomics Proteomics research using mass spectrometry encompasses several critical applications:   1. Protein Identi......

• • Mass Spectrometry in Protein Analysis: New Insights for Biopharmaceuticals and Disease Treatment

  Mass Spectrometry is an analytical technique based on molecular mass and relative abundance, which enables the determination of molecular composition, structure, and quantity. This method involves ionizing the target molecules and analyzing them in a mass spectrometer, generating a mass spectrum that provides detailed information about the molecules.   Types of Mass Spectrometry This section introduces several widely used mass spectrometry techniques, including Proton Transfer Reaction Mass Spectromet......

• • Mass Spectrometry-Based Proteomics and Metabolomics Analysis Techniques

  Mass Spectrometry-Based Proteomics and Metabolomics Analysis Techniques are two fundamental branches of systems biology that are widely used in biomedical research, disease mechanism exploration, drug development, and biomarker discovery. Both rely on mass spectrometry (MS) to identify and quantify biomolecules within biological samples; however, they focus on different types of biomolecules. While proteomics investigates proteins and their modifications, interactions, and functions, metabolomics focu......

• • Mass Spectrometry and Proteins

  Mass spectrometry analysis has been widely employed in various research areas, including the identification and quantification of proteins, the analysis of epigenetic modifications, and the investigation of protein interaction networks. This technique allows for the precise measurement of the mass of proteins or peptides, facilitating the determination of their structure and composition.   Basic Principles of Mass Spectrometry and Proteins Analysis 1. Sample Preparation Protein samples are usually sub......

• • Utilization of Unbiased Protein Identification by Mass Spectrometry in Biopharmaceutical Research

  Unbiased protein identification by mass spectrometry is a high-throughput method for protein identification that leverages mass spectrometry analysis. The technique involves isolating proteins from biological samples and analyzing them through mass spectrometry to identify and quantify the protein components present. Compared to traditional methods, this approach offers superior accuracy and sensitivity, with the added advantage of simultaneously identifying a large number of proteins.   Principle of ......

• • Mass Spectrometry-Based Protein Identification Case Study

  Mass Spectrometry-Based Protein Identification Case Study Involves the Following Steps Mass spectrometry-based protein identification is a widely used technique in proteomics research. The typical workflow involves several critical steps:   1. Sample Preparation Proteins are extracted from the biological sample and enzymatically digested, usually with trypsin, to produce peptide fragments.   2. Mass Spectrometry Analysis The peptide mixtures are then analyzed using liquid chromatography-tandem mass sp......

• • Protein Analysis via Mass Spectrometry

  Protein analysis via mass spectrometry is a widely used technique in proteomics and includes the following steps: Sample Preparation Proteins are extracted and purified through methods such as gel electrophoresis and liquid chromatography.   Protein Digestion Proteins are enzymatically digested into peptide fragments, typically using enzymes like trypsin.   Mass Spectrometry Analysis The peptide fragments are ionized, with common methods being MALDI and ESI. Their mass-to-charge ratio (m/z) is then me......

• • What Sample Types Can be Analyzed by Protein Mass Spectrometry?

  Protein mass spectrometry is a powerful analytical tool used to study various biological samples, with applications ranging from protein expression profiling to interaction mapping. The following sections outline the main sample types suitable for this technique and their corresponding research applications.   Cells and Tissues Cells and tissues are among the most commonly analyzed samples for protein mass spectrometry. Proteins can be extracted from these sources using physical or chemical methods. T......

• • How Can High-Resolution Protein Mass Spectrometry be Used to Analyze the Composition of Complex Samples?

  High-resolution protein mass spectrometry (HR-PMS) has emerged as a transformative analytical tool in modern proteomics, leveraging the principles of ionization and separation to measure the mass and relative abundance of ions with exceptional accuracy. The defining advantage of HR-PMS lies in its unparalleled capability to achieve precise mass measurements, enabling detailed characterization of complex protein samples. Techniques such as mass spectrometry (MS) and tandem mass spectrometry (MS/MS) are......