Resources

Proteomics Databases



Metabolomics Databases

• • LC-MS Protein Identification

  LC-MS protein identification is a technique for the identification and quantification of proteins using liquid chromatography-mass spectrometry (LC-MS). In proteomics research, LC-MS protein identification is invaluable. LC-MS technology integrates the separation ability of liquid chromatography with the sensitivity of mass spectrometry, allowing for the rapid and efficient identification of proteins in complex biological samples. Through LC-MS protein identification, researchers can determine protein......

• • Label-Free Quantification Mass Spectrometry

  Label-free quantification mass spectrometry (LFQ-MS) is an advanced technique for protein quantification that operates without the use of isotopic or chemical labels. Traditional methods of protein quantification often require the addition of labels during sample preparation to distinguish between proteins from different sources during analysis. Label-free quantification mass spectrometry simplifies this by directly analyzing the mass spectrometric data of protein peptides. By comparing the ion intens......

• • Label-Free Quantitative Proteomics in Protein Interactions

  Label-free quantitative proteomics is an effective technique for analyzing and comparing protein expression levels in biological samples without using isotopes or chemical labels. Utilizing high-resolution mass spectrometry, this approach enables the direct acquisition of comprehensive data on thousands of proteins from complex biological samples. Its primary advantage lies in its label-independent sensitivity and accuracy, making it ideal for various biomedical research applications.   In practice, l......

• • Olink Proteomics Panel Selection

  The Olink proteomics panel is a high-throughput, high-sensitivity biomolecular analysis tool that enables the simultaneous analysis of hundreds of proteins in a single run. Selecting the most suitable panel can facilitate efficient data acquisition and interpretation for researchers. This guide provides detailed assistance to help you identify and select the optimal Olink proteomics panel for your research needs.

• • Ion Exchange Chromatography Protein

  Ion Exchange Chromatography Protein Analysis is a commonly employed technique in protein separation and purification. This method capitalizes on differences in the surface charge of protein molecules to separate them using ion exchange chromatographic media. The fundamental principle involves utilizing the electrostatic attraction between charged stationary phases and protein molecules with opposite charges. Renowned for its efficiency and selectivity, this method finds widespread application in bioch......

• • Exosome Proteomics Sample Preparation

  1. Sample Collection: First, samples containing exosomes must be collected. This typically involves gathering from sources such as cell culture media, blood, urine, and cerebrospinal fluid, depending on the research objectives. For instance, tumor cell culture media is often collected for cancer studies. 2. Centrifugation and Ultracentrifugation: Once the samples are collected, exosomes are isolated through a series of centrifugation and ultracentrifugation steps. Initially, low-speed centrifugation........

• • How Many Proteins Can Be Detected by TMT in Proteomics

  Tandem Mass Tag (TMT) is a widely used high-throughput protein quantification technique in proteomics, designed to investigate protein expression differences across various samples. TMT enables the quantitative comparison of corresponding proteins between samples by labeling peptides with specific tags following protein hydrolysis.

• • What Is Used to Identify the Modification of a Specific Protein

  Specific protein modifications can be identified using a variety of methodologies, including: 1. Mass Spectrometry Fingerprinting This is a robust technique employed to identify proteins and their modifications. By examining the mass and distribution of proteins or peptide fragments, specific post-translational modifications such as phosphorylation, glycosylation, and ubiquitination can be accurately identified.

• • Which Type of Mass Spectrometry Is Best for Proteomics Identification

  Identification of protein modifications: LC-MS/MS can identify various chemical modifications of proteins, such as phosphorylation, acetylation, and ubiquitination, providing crucial information for studying protein function and regulatory mechanisms.

• • Principle of Mass Spectrometry for Detecting Ubiquitination

  The ubiquitin-proteasome pathway constitutes a major mechanism of protein degradation and plays a critical role in maintaining intracellular protein homeostasis. Ubiquitination, a post-translational modification, involves the covalent attachment of the small protein ubiquitin to target substrates. Mass spectrometry has emerged as a powerful and indispensable tool for investigating ubiquitination. In the following sections, we provide a detailed examination of the fundamental principles underlying the.......