Resources

Proteomics Databases



Metabolomics Databases

• • Tandem Mass Spectrometry Protein Sequencing

  Tandem mass spectrometry protein sequencing is a pivotal technique for determining the sequence and structural characteristics of protein molecules. By integrating liquid chromatography with mass spectrometry, it enables the identification and quantification of proteins within complex biological samples. The fundamental principle involves initially fragmenting protein samples into smaller peptides using a mass spectrometer, followed by further fragmentation of these peptides to glean detailed structur......

• • Charge Variant Analysis

  Charge variant analysis is an important method in proteomics, primarily used to investigate the subtle structural variations of proteins and their impacts on biological function. As key molecules in life processes, the structure and charge state of proteins influence their physiological functions in organisms. During protein synthesis and post-translational modifications, various charge variants may arise. While these variants are nearly identical in amino acid sequence, they may differ in isoelectric......

• • Host Cell Protein Analysis in Biopharmaceuticals

  Host cell protein analysis is an essential technique in the biopharmaceutical industry, primarily aimed at detecting and analyzing residual host cell proteins in biopharmaceutical products. These proteins, expressed by host cells such as E. coli, yeast, or mammalian cells during biopharmaceutical production, are non-product-related. When recombinant DNA technology is used to produce a drug, residual host cell proteins are inevitably present, often in trace amounts. The primary objective of host cell p......

• • Protein Purification and Characterization

  Protein purification and characterization is a fundamental aspect of modern life sciences research. It involves a range of advanced techniques to isolate target proteins from complex biological samples and conduct comprehensive analyses of their physicochemical properties, structural characteristics, and functions. As essential molecules in life processes, proteins participate in nearly every biological function. Through systematic purification and characterization, researchers can accurately delineat......

• • Protein Qualitative Analysis

  Protein qualitative analysis is a crucial technique in proteomics research, aimed at identifying the protein composition of complex biological samples and analyzing their structural and functional properties. As key molecules in biological processes, proteins carry out a wide range of functions in organisms, such as catalyzing chemical reactions, transmitting signals, and maintaining cell structure. Utilizing advanced analytical techniques, protein qualitative analysis helps researchers identify the s......

• • Protein-Protein Interaction Detection Methods and Analysis

  Protein-Protein Interaction Detection Methods and Analysis are crucial components of life sciences research, designed to uncover the mechanisms of interaction between proteins and other molecules, such as nucleic acids, small molecules, and ions, and to understand their functional roles. Protein-protein interactions are essential for intracellular processes such as signal transduction, metabolic regulation, and the maintenance of physiological functions. Investigating these interactions provides valua......

• • Protein Profiling in Plants

  Protein profiling in plants is a high-throughput mass spectrometry-based technique used to study the composition, expression levels, post-translational modifications, and functional properties of plant proteins. As a critical component of proteomics research, protein profiling in plants provides essential data to uncover mechanisms underlying plant growth, development, metabolism, and environmental responses. Unlike traditional methods, this approach comprehensively addresses the complexity of plant p......

• • Protein Profiling by SDS-PAGE

  Protein profiling by SDS-PAGE is a widely utilized experimental technique grounded in polyacrylamide gel electrophoresis, essential for protein research. SDS (sodium dodecyl sulfate) is an anionic surfactant that denatures proteins by disrupting their native structures and imparts uniform negative charges, ensuring that protein separation during electrophoresis is determined solely by molecular weight. PAGE (polyacrylamide gel electrophoresis) provides a polymeric gel matrix optimized for high-resolut......

• • Protein and Nucleotide Sequence Analysis

  Protein and nucleotide sequence analysis is a foundational technology in life sciences, enabling researchers to unravel how genetic information encodes proteins and dictates their functions. Nucleotide sequences govern the arrangement of amino acids in proteins, which in turn determine their three-dimensional structures and functional properties. Protein and nucleotide sequence analysis not only provides a basis for decoding the genetic code but also serves as an indispensable tool in understanding di......

• • N-Terminal Analysis

  N-terminal analysis, the determination of the N-terminal amino acid sequence, is employed to identify and characterize the sequence of proteins or polypeptides. The N-terminus, the end of a protein or polypeptide with a free amino group, plays a pivotal role in protein biosynthesis and functionality. This technique has wide-ranging applications in protein identification, post-translational modification studies, and biomarker discovery. In the development of biopharmaceuticals, N-terminal analysis help......