Recombinant Collagen Identification Service

Recombinant Collagen Identification Service is a specialized analytical service designed to confirm the structural accuracy and completeness of recombinant collagen expression products at the molecular level. Centered on mass spectrometry, this service employs techniques such as peptide mapping, N-/C-terminal sequence analysis, and molecular weight determination to identify and validate recombinant collagen. It is applicable to structural and functional research, biomedical material development, and the establishment of quality standards for recombinant collagen.

 

Collagen is the most abundant structural protein in animals, widely present in connective tissues such as skin, tendons, bones, cartilage, blood vessels, and basement membranes. It plays essential biological roles in maintaining tissue mechanical integrity, promoting cell adhesion and migration, and regulating cell growth and differentiation. With the advancement of tissue engineering, regenerative medicine, and functional biomaterials, there is increasing demand for high-quality collagen materials with low immunogenicity and controlled structure.

 



Davison-Kotler E. et al. Bioengineering. 2019.

 

To replace traditional animal-derived collagen, Recombinant Collagen has emerged as a promising alternative. Produced via genetic engineering in systems such as E. coli, yeast, mammalian cells, or insect cells, recombinant collagen offers excellent biocompatibility, controlled structure, and low immunogenicity. It is widely used in medical dressings, tissue scaffolds, skincare products, bio-inks, and novel drug delivery systems. However, due to its multi-chain assembly structure and susceptibility to expression systems, processing methods, and post-translational modifications, comprehensive and systematic molecular-level identification is essential to ensure product quality and functional performance.

 

Leveraging advanced chromatography and high-resolution mass spectrometry platforms, MtoZ Biolabs provides Recombinant Collagen Identification Service based on multi-dimensional methods such as peptide mapping, N/C-terminal sequencing, and molecular weight determination to analyze the expression product composition, sequence integrity, and batch consistency of recombinant collagen, providing customers with accurate information support at all stages of R&D, production, registration, and quality control.

 

Recombinant Collagen Peptide Mapping Analysis Service

Utilizes HPLC-based peptide mapping or UPLC-MS-based peptide mass mapping to identify recombinant collagen. This service helps confirm the integrity, sequence consistency, and post-translational modification (PTM) information of the protein.

 

Recombinant Collagen N-terminal Sequence Analysis Service

Employs Edman degradation or mass spectrometry to accurately analyze the N-terminal amino acid sequence of recombinant collagen. This service supports verification of product quality and stability, helping to prevent potential structural issues and providing a reliable foundation for downstream biological applications.

 

Recombinant Collagen C-terminal Sequence Analysis Service

Uses mass spectrometry to precisely analyze the C-terminal amino acid sequence of recombinant collagen. This service assists in validating structural completeness, identifying post-translational modifications, and confirming functional relevance.

 

Recombinant Collagen High-Resolution Molecular Weight Identification Service

Determines the accurate molecular weight of recombinant collagen using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and Electrospray Ionization Mass Spectrometry (ESI-MS).

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Recombinant Collagen Identification Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. Comprehensive Structural Coverage: Integrates multiple mass spectrometry strategies to systematically analyze the peptide map, sequence, and molecular weight of collagen.

4. Cross-System Compatibility: Applicable to a wide range of expression systems, including E. coli, yeast, and mammalian cells.

5. Expandable Service Modules: Can be seamlessly integrated with molecular weight analysis, peptide mapping comparison, and terminal sequencing services.

 

Sample Submission Suggestions

MtoZ Biolabs accepts a variety of sample types, including purified recombinant collagen, lyophilized powders, and more. Samples should be shipped at low temperatures and protected from repeated freeze-thaw cycles.

If your sample conditions are special or if you're unsure how to prepare your samples, please feel free to contact the MtoZ Biolabs technical team. We will provide personalized sample submission guidelines and pretreatment support based on your needs.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files