Recombinant Collagen High-Resolution Molecular Weight Identification Service

Recombinant Collagen High-Resolution Molecular Weight Identification Service is a specialized service for determining the molecular weight of recombinant collagen using high-resolution techniques. By combining matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and electrospray ionization mass spectrometry (ESI-MS), this service enables accurate mass determination from small peptide fragments to high-molecular-weight polymers.

Recombinant collagen has emerged as a critical protein material in tissue engineering, regenerative medicine, cosmetic skincare, and biomaterials due to its excellent biocompatibility, tunable structural properties, and broad functional potential. Given its large molecular size, diverse subtypes, triple-helix structure, and polymeric assembly, precise molecular weight (MW) assessment is essential for ensuring its quality, functional performance, and batch-to-batch consistency. High-resolution mass spectrometry is an indispensable technique in modern protein and peptide characterization and is particularly suited for molecular weight determination of recombinant collagen. Among mass spectrometric techniques, MALDI-TOF allows direct measurement of molecular weight, while ESI-MS provides multiply charged ion signals, which are deconvoluted to obtain the accurate molecular mass.

 



Suzuki MF. et al. AMB Expr. 2020.

 

Based on MALDI-TOF-MS and ESI-MS platforms, MtoZ Biolabs offers Recombinant Collagen High-Resolution Molecular Weight Identification Service enabling precise molecular weight analysis of collagen monomers and their polymers ranging from tens to several thousand Daltons. This service can verify whether recombinant collagen is fully expressed, detect the presence of degradation products or cross-linked byproducts, and identify possible subtype mixtures, providing critical data support for raw material quality control, process validation, and regulatory submissions.

 

Analysis Workflow

Recombinant Collagen High-Resolution Molecular Weight Identification Service follows a standardized and traceable analytical workflow:

1. Sample Quality Assessment

Evaluate sample purity and concentration.

 

2. Buffer Exchange and Sample Pretreatment

Perform buffer exchange using ultrafiltration or desalting columns to remove non-volatile salts, surfactants, and other components that may interfere with the ionization process.

 

3. MALDI or ESI Platform Analysis

Select the appropriate ionization method and mass spectrometry platform based on the molecular weight range and polymerization state of the sample; acquire full-spectrum mass data.

 

4. Deconvolution and Data Processing

Use specialized software to deconvolute multiply charged spectra, calculate the exact molecular weight of a single conformation, and verify results with experimental data.

 

5. Data Delivery and Report Output

Deliver a complete technical report including raw spectra, deconvolution results, molecular weight statistics, peak area ratios, and error margins.

 

Service Advantages

1. Dual-Platform Support: Flexible selection between MALDI and ESI-MS to cover a wide range of molecular weights and conformational states.

2. High-Resolution Accuracy: Mass spectrometers offer ppm-level resolution with mass error controlled within ±1 Da.

3. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

4. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Recombinant Collagen High-Resolution Molecular Weight Identification Service data, providing clients with a comprehensive data report.

 

Sample Submission Suggestions

MtoZ Biolabs accepts a variety of sample types, including purified recombinant collagen, lyophilized powders, and more. Samples should be shipped at low temperatures and protected from repeated freeze-thaw cycles.

If your sample conditions are special or if you're unsure how to prepare your samples, please feel free to contact the MtoZ Biolabs technical team. We will provide personalized sample submission guidelines and pretreatment support based on your needs.

 

Applications

Recombinant Collagen High-Resolution Molecular Weight Identification Service is primarily used for:

Verification of Expression Product Integrity

Assess whether the target molecule is fully expressed and detect abnormalities such as N-/C-terminal truncations, extensions, or misassembled multimers.

 

Analysis of Multimers and Aggregates

Determine the distribution of monomers, dimers, trimers, etc., and evaluate the impact of native or non-specific aggregation on product quality.

 

Batch Consistency and Quality Control

Perform precise molecular weight comparison across different expression batches to support stability evaluation of pharmaceutical raw materials or final products.

 

Deliverables

1. Experimental Steps

2. Relevant Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Recombinant Collagen Molecular Weight Information