Peptide Mapping Analysis Service | Recombinant Collagen

Peptide Mapping Analysis Service of Recombinant Collagen refers to the service of identifying recombinant collagen using HPLC peptide mapping or UPLC mass peptide mapping analysis technology. It can help confirm the integrity, sequence consistency and post-translational modification information of recombinant collagen.

 

Recombinant collagen is a collagen produced in host cells (such as E. coli, yeast or mammalian cells) through genetic engineering technology. Because it can mimic the structure and function of natural collagen, recombinant collagen is widely used in tissue engineering, wound repair, skin care and drug delivery systems. Compared with natural collagen, recombinant collagen has higher purity and controllability, but due to the complexity of its production process, ensuring its structural integrity and functional stability is the key to its successful application.

 



Millán-Martín S. et al. Anal Bioanal Chem. 2020

 

Peptide Mapping Analysis technology can be used to ensure the quality of collagen. It uses specific chemical reagents or enzymes to specifically degrade proteins into peptides, which are then separated and identified by reliable methods, and compared with reference products treated by the same method. Depending on the detection instrument used, peptide mapping can be divided into peptide mapping based on high performance liquid chromatography and mass peptide mapping based on mass spectrometry. It is a key step in the characterization of biological products and an important means of quality control.

 

Leveraging advanced chromatography and mass spectrometry platforms, MtoZ Biolabs provides Recombinant Collagen Peptide Mapping Analysis Service performing peptide mapping or mass peptide mapping analysis on recombinant collagen to help customers ensure the quality and function of recombinant collagen. Whether you are engaged in basic research, product development, or quality control, we can provide strong technical support for your work.

 

Analysis Workflow



Lam AK. et al. Mol Ther Methods Clin Dev. 2022.

 

The main steps of Recombinant Collagen Peptide Mapping Analysis Service are as follows:

1. Enzymatic hydrolysis and separation

Use trypsin and other enzymes to hydrolyze collagen to generate peptides.

 

2. Peptide analysis

Use HPLC or UPLC-MS to analyze peptides and obtain chromatograms and sequence information of peptides.

 

3. Data analysis and comparison

Draw peptide coverage and total sequence diagram of peptides, and compare with theoretical information or standards to confirm the integrity, purity and post-translational modification information of recombinant collagen.

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Peptide Mapping Analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Recombinant Collagen Peptide Mapping Analysis data, providing clients with a comprehensive data report.

4. Flexible Service Customization: Tailored analytical services are provided based on the client's specific needs.

 

Sample Submission Suggestions

MtoZ Biolabs can accept a variety of sample types, including purified recombinant collagen and lyophilized powder. All samples should be kept frozen and avoid repeated freezing and thawing.

 

If your sample conditions are special or you are not sure how to prepare, please feel free to contact the technical team of MtoZ Biolabs. We will provide personalized sample submission guidelines and pre-processing support according to your needs.

 

Applications

Application examples of the Recombinant Collagen Peptide Mapping Analysis Service:

Biopharmaceuticals and Tissue Engineering: Validate the amino acid sequence of recombinant collagen to confirm its consistency with natural collagen, ensuring its biocompatibility and efficacy in tissue repair.

Skincare and Anti-Aging Products: Ensure the quality of recombinant collagen to avoid issues with structural instability.

Immunology Research and Vaccine Development: Determine the antigenicity of recombinant collagen in vaccines, ensuring it can activate an effective immune response.

Drug Delivery Systems: Verify the stability of recombinant collagen under different conditions and assess its potential as a drug carrier.

 

Deliverables

1. Experimental Steps

2. Related Instrument Parameters

3. Detailed Peptide Mapping Information

4. Chromatography/Mass Spectrometry Images

5. Raw Data