Protein Identification (In-Gel, Spot, Solution) Service
In modern life science research, proteins are the core molecules that execute biological functions, widely involved in metabolic regulation, signal transduction, cellular structure, and immune response. Accurate identification of protein species and characteristics in a sample is crucial for understanding biological mechanisms, discovering biomarkers, and validating therapeutic targets. Using mass spectrometry, proteins can be digested into peptides, analyzed to generate characteristic spectra, and compared against databases for precise sequence identification. MtoZ Biolabs offers Protein Identification (In-Gel, Spot, Solution) Service that assists researchers in analyzing SDS-PAGE gel bands/spots, solution-based protein samples, or simple mixtures while ensuring cost-effective and reliable protein identification support.
Figure 1. Workflow of Protein Identification
Services at MtoZ Biolabs
We utilize advanced mass spectrometry platforms, including LC-MS/MS, ESI-MS, and MALDI-TOF/TOF for protein identification across diverse sample types. Our protein identification (in-gel, spot, solution) service coverage includes:
1. Mass spectrometry-based identification of SDS-PAGE gel bands or targeted spots.
2. Protein identification from solution samples.
3. Complex Mixture Protein Identification.
4. Identification and characterization of unknown proteins in mixtures.
5. Protein Identification from Pull-Down and Co-Immunoprecipitation Samples.
These capabilities allow us to handle a wide range of sample types, from purified single proteins to complex backgrounds.
Analysis Workflow
1. Sample Reception and Evaluation: Confirm sample type (gel band/spot or solution) and assess concentration, purity, and other basic parameters.
2. Pre-treatment: Gel samples undergo destaining, reduction, alkylation, and enzymatic digestion; solution samples are directly digested.
3. Mass Spectrometry Analysis: Acquire MS/MS spectra via LC-MS/MS or MALDI-TOF/TOF.
4. Data Processing and Database Search: Match peptides against protein databases using professional software to generate reliable identification results.
5. Result Review and Delivery: Conduct quality control and deliver a report including identified proteins, peptide spectra, and confidence scores.
Service Advantages
1. Comprehensive Instrumentation
Equipped with LC-MS/MS, ESI-MS, and MALDI-TOF/TOF, we flexibly accommodate different sample types and research needs, ensuring robust data quality and accurate identifications, along with spectrum support and tailored solutions.
2. Broad Sample Compatibility
Our protein identification (In-Gel, Spot, Solution) service covers SDS-PAGE gel bands and spots, solution samples, pull-down/Co-IP products, and mixed protein samples. From purified proteins to complex samples, we deliver efficient identification and save researchers valuable time and resources.
3. Transparent Pricing and Rapid Turnaround
We provide clear and transparent service pricing with no hidden costs. Projects are initiated promptly, and typical turnaround times are short, enabling researchers to meet tight experimental timelines.
4. Standardized Workflow and High-Throughput Compatibility
Strict SOPs are followed from sample handling through reporting, ensuring reproducibility and consistency. Our workflow supports both small-scale exploratory studies and large-scale projects.
Applications
1. Target Discovery and Validation: Identify differentially expressed or modified proteins in disease models to support target confirmation and mechanistic studies.
2. Protein–Protein Interaction Studies: Identify binding partners from Co-IP or pull-down assays to build protein interaction networks.
3. Functional Protein Analysis: Extract and identify proteins from SDS-PAGE spots for structural and functional research.
4. Biomarker Discovery: Identify proteins in serum, tissues, or cell extracts to support preclinical biomarker discovery.
FAQs
Q1: What is the minimum sample amount required?
A1: We recommend a total protein amount ≥ 5 μg and concentration ≥ 0.1 μg/μL. For gel samples, bands or spots should be clearly stained with relatively high purity.
Q2: Which staining method is recommended?
A2: Coomassie Brilliant Blue is preferred. Silver staining is also acceptable, though Coomassie-stained gels are more suitable for downstream identification.
Q3: How should samples be submitted?
A3: Please provide gel images along with the actual gel bands/spots in microtubes with a small amount of ddH₂O for preservation, and include sample details such as origin, concentration, and volume.
Sample Submission Suggestions
1. Gel Samples: Coomassie- or silver-stained bands or spots with purity ideally >90%. Submit gel photos along with samples.
2. Solution Samples: Single proteins or lightly mixed samples with ≥ 5 μg total protein, concentration ≥ 0.1 μg/μL, and preferably >90% purity.
3. Submission Format: Samples should be placed in clean, nuclease- and protease-free tubes with a small volume of ddH₂O. Include sample details such as concentration, volume, origin, and preparation method.
Deliverables
1. A standardized identification report (PDF/Word) including methods, identified proteins, peptide match information, scores, and QC notes.
2. Raw mass spectra (optional) and peptide match tables.
3. Data interpretation suggestions (e.g., interaction network or functional enrichment overview upon request).
If you are seeking a reliable, flexible, and professional protein identification solution, MtoZ Biolabs’ protein identification (in-gel, spot, solution) service is your optimal choice. With advanced instrumentation and an experienced team, we are dedicated to understanding your research needs and providing practical scientific support.
Contact us today for a free consultation and quotation to advance your project with confidence!
Related Services
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