High-Resolution MS Molecular Weight Identification Service

    At present, there are two types of mass spectrometry used for molecular weight analysis: Matrix-assisted laser desorption/ionization (MALDI-TOF) and Electrospray ionization (ESI-MS). The two methods have their own advantages and applications. MALDI-TOF can be used to directly measure the molecular weight of biopharmaceuticals, while ESI-MS analyzes multiple charge signals obtained by electric spray, and speculates the precise molecular weight through deconvolution. MALDI-TOF-MS is more suitable for analyzing biopharmaceuticals with molecular weight less than 25kDa. ESI-MS is recommended for molecular weight identification of protein substances with molecular weight of larger than 25kDa. MtoZ Biolabs has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

     

    MtoZ Biolabs has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

    Figure 1. MALDI-TOF Analysis of Biopharmaceuticals

     

    MtoZ Biolabs has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

    Figure 2. ESI-MS Analysis of Biopharmaceuticals

     

    Sample Submission Requirements 

    1. Sample Requirement

    The sample should be dissolved in the volatile solvent with a low content of salt and detergent.

     

    2. Requirement for Concentration of Detergents

     

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    3. Transportation of Samples

    Freeze-dried protein samples, transported at frozen or 4 ℃.

     

    Case Study

    MALDI can analyze molecular weight of protein sample and the presence of polymers in protein or peptide segments in protein. The following is an actual result of determination of the molecular weight of a protein drug. We can see from the graph that there are dimers and trimmers existing in the protein sample.

     

    MtoZ Biolabs has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.

     

    Deliverables 

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Molecular Weight Results

    5. Bioinformatics Analysis

     

    Related Services

    Identification of Biopharm

    N/C Terminal Sequencing

    Peptide Mapping

    Amino Acids Composition

     

    Variation Analysis

    Glycosylation Site Analysis

    Disulfide Bond Analysis

     

    Purity Analysis

    Purity Analysis SDS-PAGE

    Purity Analysis SEC and RPLC

    Host Cell Protein (HCP) Analysis

    Antibody-Drug Conjugate (ADC) Analysis

  • • SEC-MALS Molecular Weight Determination Service

    Molecular weight is one of the main characteristic parameters of proteins, which is an important prerequisite for determining a protein and carrying out subsequent research activities of proteins. Therefore, it is important to accurately and efficiently determine the molecular weight of proteins.

  • • High-Resolution LC-MS Molecular Weight Identification Service

    Molecular weight, as a key characteristic parameter of proteins, is crucial for identifying specific proteins and conducting subsequent protein research activities. Therefore, accurately and effectively determining the molecular weight of proteins is particularly important. Currently, the common methods for identifying molecular weight include gel electrophoresis, MALDI-TOF, LC-MS, SEC-MALS, etc.

  • • MALDI TOF Molecular Weight Identification Service

    Molecular weight, as a key characteristic parameter of proteins, is crucial for identifying specific proteins and conducting subsequent protein research activities. Therefore, accurately and effectively determining the molecular weight of proteins is particularly important. Currently, the common methods for identifying molecular weight include gel electrophoresis, MALDI-TOF, LC-MS, SEC-MALS, etc. The advantages of MALDI over other methods include short detection time, low consumption, low solvent requiremen

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