Molecular Weight Analysis
Overview
At present, there are two types of mass spectrometry used for molecular weight analysis: Matrix-assisted laser desorption/ionization (MALDI-TOF) and Electrospray ionization (ESI-MS). The two methods have their own advantages and applications. MALDI-TOF can be used to directly measure the molecular weight of biopharmaceuticals, while ESI-MS analyzes multiple charge signals obtained by electric spray, and speculates the precise molecular weight through deconvolution. MALDI-TOF-MS is more suitable for analyzing biopharmaceuticals with molecular weight less than 25kDa. ESI-MS is recommended for molecular weight identification of protein substances with molecular weight of larger than 25kDa. MtoZ Biolabs has established a platform with both MALDI-TOF and ESI-MS technologies, which can be used to determine the molecular weight of all kinds of biopharmaceuticals, including antibodies, vaccines, proteins drugs, and polypeptides.
MALDI-TOF Analysis of Biopharmaceuticals 
ESI-MS Analysis of Biopharmaceuticals
Sample Requirements
Sample requirement: the sample should be dissolved in the volatile solvent with a low content of salt and detergent.
Requirement for concentration of detergents
Transportation of samples: freeze-dried protein samples, transported at frozen or 4 ℃.
Case Study
MALDI can analyze molecular weight of protein sample and the presence of polymers in protein or peptide segments in protein. The following is an actual result of determination of the molecular weight of a protein drug. We can see from the graph that there are dimers and trimmers existing in the protein sample.
Reports
• Experiment procedures
• Parameters of liquid chromatography and mass spectrometer
• MS raw data files
• Molecular weight results
• Bioinformatics analysis
Related Services
N/C Terminal Sequencing
Peptide Mapping
Amino acids composition
Glycan Profiling
Glycosylation site analysis
Disulfide bond analysis
Antibody C-terminal variation analysis
Purity analysis SDS-PAGE
Purity analysis SEC and RPLC
Host Cell Protein (HCP) Analysis
Antibody-Drug Conjugate (ADC) Analysis
How to order?
