Disulfide Bond Analysis Service

    Disulfide bond (S-S bond) is formed by oxidation of the sulfhydryl group (-SH) on two cysteines in the protein. It is an important post-translational modification of protein. Disulfide bond is essential for protein molecules to maintain the correct advanced structure and maintain protein bioactivity. The distribution of disulfide bonds in antibody drugs is a direct structural characteristic of the drugs. Therefore, confirmation of disulfide bonds plays a very important role in the confirmation process of antibody drug structure. MtoZ Biolabs has developed a high-resolution mass spectrometry, coupled with pLink-SS software, to provide our customers with accurate analysis of disulfide bonds and free cysteines. Our sample preparation steps have also been optimized to prevent in vitro exchange of disulfide bonds, and maintain native structure.

     

    MtoZ Biolabs has developed a high-resolution mass spectrometry, coupled with pLink-SS software, to provide our customers with accurate analysis of disulfide bonds and free cysteines.

    Figure 1. Disulfide Bond Analysis of Biopharmaceuticals

     

    Applications 

    1. Identification of the Number of Disulfide Bonds in the Biopharmaceuticals and Free Cysteines

    2. Identification of the Position of Disulfide Bonds in the Biopharmaceuticals

     

    Deliverables

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Disulfide Bonds and Free Cysteines Analysis Results

    5. Bioinformatics Analysis

     

    Related Services

    Identification of Biopharm

    Molecular Weight Analysis

    N/C Terminal Sequencing

    Peptide Mapping

    Amino Acids Composition

     

    Variation Analysis

    Glycosylation Site Analysis

     

    Purity Analysis

    Purity Analysis SDS-PAGE

    Purity Analysis SEC and RPLC

    Host Cell Protein (HCP) Analysis

    Antibody-Drug Conjugate (ADC) Analysis

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