Glycosylation Site Detection Service

    Glycosylation, especially N-glycosylation, is a universal post-translational modification in regarding the localization, function, activity of proteins in tissues and cells. The study of the N-glycosylation site is an important prerequisite for understanding the function of sugar chain of the target protein. Among the information related to N-glycosylation, the site of N- glycosylation is the basis for our understanding of its sugar chain function. Besides the application of point mutation in traditional biochemistry, the study of glycosylation sites has also played an important role in the identification of glycosylation sites in N-terminal. MALDI TOF MS and nano LC-ESI-MS/MS are commonly used to identify the N/O-glycosylation sites in the antibodies. MtoZ Biolabs uses both MALDI TOF MS and nano LC-ESI-MS/MS technology to provide efficient and accurate glycosylation analysis of multiple biopharmaceuticals, including proteins, antibodies, etc. Upon the sample reception, Fc domain and Fab of the antibodies are first separated, followed by digestion into peptide fragments, and analysis of glycosylation site by MALDI TOF MS and nano LC-ESI-MS/MS.

     

    MtoZ Biolabs uses both MALDI TOF MS and nano LC-ESI-MS/MS technology to provide efficient and accurate glycosylation analysis of multiple biopharmaceuticals, including proteins, antibodies, and other biological drugs.

    Figure 1. Glycosylation Site Analysis of Antibody

     

    Analysis Workflow 

    1. Digestion of Biopharmaceutical Samples by Trypsin or Other Appropriate Proteases

    2. Separation of N-Glycopeptides and O-Glycopeptides Through Chromatographic Column

    3. Deglycosylation Treatment

    4. Peptide Analysis by MALDI-TOF MS, or ESI-MS/MS

    5. Analysis of the N/O-Glycosylation Site Based on the Mass Spectra Results

    6. Comparison of the Analytical Results of Glycosylation Sites of Different Batch Samples

     

    Deliverables 

    1. Experiment Procedures

    2. Parameters of Liquid Chromatography and Mass Spectrometer

    3. MS Raw Data Files

    4. Glycosylation Site Results

    5. Bioinformatics Analysis

     

    Related Services

    Identification of Biopharm

    Molecular Weight Analysis

    N/C Terminal Sequencing

    Peptide Mapping

    Amino Acids Composition

     

    Variation Analysis

    Disulfide Bond Analysis

     

    Purity Analysis

    Purity Analysis SDS-PAGE

    Purity Analysis SEC and RPLC

    Host Cell Protein (HCP) Analysis

    Antibody-Drug Conjugate (ADC) Analysis

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