Peptide Molecular Mass Determination Service
- Structural confirmation and sequence verification of peptides
- Quality control and consistency assessment of synthetic peptides
- Mass validation of modified peptides (e.g., phosphorylation, glycosylation)
- Quality monitoring in peptide vaccine and peptide drug development
- Component analysis of protein digestion products
- Molecular mass comparison of biomarker-derived peptide fragments
Peptide Molecular Mass Determination Service is a professional service that quantitatively determines the accurate molecular mass of peptide molecules by means of high-resolution mass spectrometry. This service is of great value in many life science research fields such as peptide drug development, synthetic peptide verification, protein modification analysis, and sequence consistency confirmation.
During synthesis or experimental processes, peptides may exhibit structural errors, incomplete modifications, degradation, or coexistence with impurities. Molecular weight, as the most direct structural indicator, reflects whether the peptide chain has been correctly synthesized, whether expected chemical modifications are present, and whether any unexpected chemical changes have occurred. Accurate molecular mass determination is therefore a critical component of quality control and a foundational requirement for ensuring downstream research reliability. Peptide mass determination primarily relies on mass spectrometry to measure the precise mass-to-charge ratio (m/z) of ionized peptides. The resulting spectra are processed using deconvolution algorithms to calculate the actual molecular weight. For more complex or highly aggregated samples, additional techniques such as gel permeation chromatography (GPC/SEC), SDS-PAGE, or capillary electrophoresis can be employed to assess molecular weight distribution, further confirming peptide homogeneity or aggregation state.
Leveraging high-resolution mass spectrometry and a variety of molecular weight analysis platforms, MtoZ Biolabs offers Peptide Molecular Mass Determination Service to provide precise molecular weight measurements for a wide range of peptide types, including linear peptides, cyclic peptides, modified peptides, and natural peptides. This service supports multiple ionization methods (such as MALDI and ESI) and platform combinations, enabling researchers to quickly verify peptide integrity, modification status, and purity-laying a robust analytical foundation for peptide drug development, protein function studies, and quality control.
Kanbargi KD. et al. Food Measure. 2016.
Figure 1. Identification of molecular mass of peptide formed by alcalase by MALDI TOF spectrometer.
Analysis Workflow
The general workflow of Peptide Molecular Mass Determination Service is as follows:
1. Sample Preparation
Peptides are prepared according to the type of sample provided.
2. Purity Assessment (Optional)
Evaluate the presence of impurities or degradation products in the peptide sample.
3. High-Resolution Mass Spectrometry Detection
Select the appropriate platform (e.g., MALDI-TOF MS or ESI-LC-MS) based on the characteristics of the peptide.
4. Data Analysis and Deconvolution
Use specialized software to interpret mass spectra, obtain accurate molecular weight, and assess molecular weight distribution.
5. Result Reporting and Delivery
Compile the analysis results into a standardized report, including spectra, molecular mass data, and analytical conclusions.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Peptide Molecular Mass Determination Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. Compatible with Various Sample Types: Supports linear peptides, cyclic peptides, modified peptides, synthetic peptides, and complex mixtures; peptide sample preparation services are also available.
4. Molecular Weight Distribution Analysis Supported: Utilizes SEC and other separation platforms to assess aggregation or multi-component states.
5. Flexible Customized Service Plans: Service can be extended to include peptide sequence verification, modification analysis, and purity assessment based on project needs.
Sample Submission Suggestions
Sample Types: Supports synthetic peptides, natural peptides, recombinant expression products, or purified peptide samples.
Purity Requirements: Recommended purity ≥90%. Avoid the presence of salts, buffer components, or high concentrations of additives that may interfere with analysis.
Storage and Shipping: Store samples in lyophilized form or at low temperatures (–20 °C or below). Use dry ice or cold-chain packaging during transport, and avoid repeated freeze–thaw cycles.
Additional Notes: We recommend contacting us prior to sample submission for detailed and tailored sample preparation guidelines.
Applications
Peptide Molecular Mass Determination Service is applicable to a wide range of research and application scenarios:
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Raw Data Files
Related Services
Mass Spectrometry Molecular Weight Analysis Service
How to order?
