Peptide Mapping Analysis Service

MtoZ Biolabs offers advanced peptide mapping analysis service that enable clients to gain a detailed understanding of protein structure and function at the peptide level. By leveraging precise peptide mapping techniques, MtoZ Biolabs provides critical insights into protein sequences, post-translational modifications, mutations, and cleavage patterns. This service is widely used in proteomics, drug discovery, vaccine development, and other scientific fields, aiding researchers in the structural and functional analysis of complex biological samples.

 

Services at MtoZ Biolabs

MtoZ Biolabs’ peptide mapping analysis service is designed to deliver precise peptide sequence data, covering:

1. Protein Sequence Elucidation: Using peptide mass spectrometry, we determine the complete sequence of proteins, especially for novel or incompletely characterized proteins.

2. Post-translational Modification Identification: We identify post-translational modifications (e.g., phosphorylation, glycosylation, methylation) in proteins, providing valuable data for structural biology studies and drug target identification.

3. Protein Variant Detection: By comparing peptide profiles from different samples, we detect and map protein mutations, supporting research into disease mechanisms and antibody development.

4. Protein Digestion Profile Analysis: We generate peptide mapping data from protein digestion reactions, enabling analysis of enzyme cleavage patterns, which is essential for antibody engineering and biopharmaceutical research.

 

Analysis Workflow

 



 

Why Choose MtoZ Biolabs?

1. Advance Analysis Platform

MtoZ Biolabs' an advanced peptide mapping analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. Tailored Digestion Protocols

We offer customized enzyme digestion strategies for various protein samples, ensuring the generation of the most representative peptides and high-quality peptide maps.

 

3. Broad Sample Compatibility

Our platform accommodates various biological samples—from purified proteins to complex matrices like cell lysates, tissues, and bodily fluids—to meet diverse research needs.

 

4. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein analysis data providing clients with a comprehensive data report.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Applications

• Proteomics research
• Drug discovery and precision medicine
• Vaccine and antibody development
• Analysis of complex samples

 

Case Study

1. Peptide mapping is employed to analyze protein digestion products in detail, facilitating the confirmation and assessment of protein structural features.

 

 

Muriithi, B. et al. J. Proteome Res. 2024.

 

FAQ

Q1: How does MtoZ Biolabs ensure peptide integrity and accuracy in peptide mapping analysis?
MtoZ Biolabs ensures peptide integrity and accuracy through optimized digestion protocols combined with high-resolution LC-MS/MS technology. Depending on the sample type, we typically use enzymes such as Trypsin, Lys-C, or Chymotrypsin to ensure uniform peptide fragmentation. During analysis, LC-MS technology, along with database comparison, is used to confirm the sequence of each peptide, ensuring their accuracy and completeness, which guarantees the reliability and reproducibility of results.

 

Q2: Can peptide mapping analysis be applied to non-standard or newly discovered proteins?
Yes, the service can be applied to both standard and non-standard proteins. Using high-resolution mass spectrometry, MtoZ Biolabs can perform sequencing of unknown or newly discovered proteins, even those with unique post-translational modifications.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!