Peptide Structure Determination Service

Peptides are abundant in living organisms, actively participating in key biological processes such as hormone regulation, enzyme catalysis, and immune responses. Their spatial structure and functional features greatly influence their biological activity. Accurately determining peptide structures is essential for understanding peptide folding patterns, bioactivity, and structural features. It also informs drug development and bioengineering optimization. Peptide structure determination is valuable in biomedical research and drug development, providing insight into protein structure-function relationships, supporting drug design, and advancing vaccine research. Furthermore, it is critical in studying disease mechanisms, developing protein-based drugs, and refining biotechnology applications.

 

Analysis Workflow

1. Sample Preparation

Extract and purify target peptides using high-performance liquid chromatography (HPLC) or other suitable techniques. Desalt and concentrate purified peptides to meet structural analysis requirements.

 

2. Sequence Analysis

(1) Select appropriate enzymes to digest peptide samples based on sequence characteristics, producing fragments for mass spectrometry analysis.

(2) Use HPLC to separate enzymatically cleaved peptides.

(3) Determine peptide fragments' amino acid composition and sequence via Edman degradation or tandem mass spectrometry (MS/MS), revealing the primary peptide structure.

 

3. Structural Analysis

Measure the peptide’s ultraviolet absorption by circular dichroism (CD), identifying their internal secondary structure elements such as α-helices and β-folds.

 



 

Applications

1. Revealing Protein Structure

Peptide structure determination identifies protein structures, including secondary, tertiary, and quaternary structures.

 

2. Studying Protein Function

Since protein structure closely correlates with function, peptide structure determination illuminates these relationships.

 

3. New Drug Development

By identifying drug-target protein interactions, peptide structure determination provides crucial insights for drug design.

 

4. Disease Diagnosis and Treatment

Peptide structure determination helps identify disease-specific biomarkers, enhancing early diagnosis and treatment efficacy.

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Peptide Structure Determination Detailed Information

4. Mass Spectrometry Images

5. Raw Data