Peptide Identification Service
Peptide identification is a fundamental technique in proteomics research. By leveraging mass spectrometry, peptides in complex biological samples can be accurately identified, enabling researchers to decipher protein composition and structure, reveal post-translational modifications (PTMs), and determine protein functional states. Peptide identification provides critical insights into protein information, including molecular weight, sequence, and modification sites. This, in turn, facilitates the discovery of disease biomarkers, the identification of drug targets, and the exploration of disease mechanisms.
Figure 1. Workflow of Peptide Identification by Database Search
Equipped with advanced mass spectrometry platforms and a professional scientific team, MtoZ Biolabs is committed to delivering efficient and accurate peptide identification service for researchers with minimal sample consumption. Utilizing the latest instrumentation and optimized analytical workflows, we are capable of extracting and identifying peptides from diverse biological matrices, providing reliable data support for your research projects.
Service at MtoZ Biolabs
MtoZ Biolabs’ peptide identification service is based on modern liquid chromatography-tandem mass spectrometry (LC-MS/MS). We utilize high-resolution instruments such as the Orbitrap Fusion Lumos and Q Exactive HF, in combination with highly efficient nano-LC systems. After enzymatic digestion of proteins, the resulting peptides are separated and analyzed. The mass-to-charge ratio (m/z) of the peptides is matched against curated databases, enabling precise identification of sequences and source proteins. Our peptide identification service includes:
1. Peptide Identification: Comprehensive peptide identification through both database search and de novo identification in the sample.
2. Post-Translational Modification Analysis: Identification of PTMs on peptides such as phosphorylation, acetylation, etc.
3. Protein Expression Profiling: Quantitative analysis of peptides to construct protein expression profiles under different conditions.
4. Custom Analysis: Tailored peptide identification solutions based on specific research needs across diverse biological contexts.
Analysis Workflow
1. Sample Preparation: Protein extraction and enzymatic digestion according to the sample type provided by the client.
2. Peptide Separation: Separation of peptides from complex biological matrices via liquid chromatography.
3. Mass Spectrometry Analysis: High-resolution MS analysis (e.g., Orbitrap Fusion Lumos) to acquire m/z information of the peptides.
4. Data Processing and Identification: Advanced bioinformatics tools are used to interpret raw MS data and identify peptides through database matching.
5. Report Generation: A detailed report is generated, including peptide sequences, protein annotations, PTM information, and quantitative data.
Service Advantages
1. High-Resolution Mass Spectrometry Platforms
MtoZ Biolabs utilizes cutting-edge mass spectrometers such as the Orbitrap Fusion Lumos and Q Exactive HF, integrated with nano-LC systems, to ensure high sensitivity and resolution in peptide identification. These platforms allow for the detection of low-abundance peptides with high confidence.
2. Comprehensive Peptide Profiling
In addition to standard peptide identification, we offer extensive PTM analysis, including phosphorylation, acetylation, ubiquitination, and lactylation. This enables a more comprehensive understanding of the proteome in your samples.
3. Efficient and Reliable Data Processing
Our experienced data analysis team is proficient in a variety of software tools and bioinformatics pipelines. We ensure fast turnaround and accurate interpretation of complex mass spectrometry datasets.
4. Customized Service Options
We provide flexible, project-specific solutions tailored to your research objectives. Whether identifying peptides in whole proteomes or targeting specific peptide biomarkers, we can customize our approach to meet the unique needs of your study, ensuring precise and reliable results.
5. One-Time Charge
Our pricing model is transparent and straightforward, with no hidden fees or additional costs, ensuring a clear and predictable budget for your project.
Applications
1. Disease Mechanism Research: Peptide identification plays a key role in understanding disease mechanisms by identifying disease-associated proteins and PTMs, offering novel therapeutic targets and biomarker candidates.
2. Drug Development: Peptide identification service is useful for identifying potential drug targets, understanding drug mechanisms, and evaluating drug effects at the proteomic level.
3. Biomarker Discovery: Identification of disease-specific proteins through peptide profiling aids in discovering new biomarkers for clinical diagnostics and early disease detection.
FAQs
1. What types of samples can I submit?
We accept a variety of sample types, including tissues, cells, serum, and plasma. Specific handling instructions may vary. Please contact our technical team for detailed submission guidelines.
2. What is the typical turnaround time for peptide identification service?
Generally, peptide identification can be completed within 2–3 weeks, depending on sample complexity and analytical requirements. We will keep you updated throughout the process.
Sample Submission Suggestions
1. Sample Types: Tissues, cells, serum, plasma, etc.
2. Sample Quantity: Typically 10–50 mg depending on the analysis requirements.
3. Sample Preparation: Protein extraction and digestion can be provided upon request.
4. Packaging: Ensure samples are well-sealed to prevent contamination or loss during transport.
Deliverables
1. Experimental Report: Includes detailed peptide identification results, PTM data, and protein expression information.
2. Data Files: Raw mass spectrometry data, processed results, and graphical representations such as heatmaps and proteomic profiles.
3. Quantitative Analysis Report: Upon request, quantitative peptide data and visual summaries are provided.
As a leading provider of proteomics services, MtoZ Biolabs leverages advanced mass spectrometry platforms and extensive expertise to deliver precise and efficient peptide identification. Whether your goal is to identify endogenous peptides in proteomic studies or to perform large-scale protein profiling, our experienced scientists and technical team are dedicated to delivering high-quality, reproducible results.
If you are interested in our peptide identification service or have any questions, please feel free to contact us. We look forward to collaborating with you and supporting your research with dependable and insightful data.
Related Services
Peptide Mass Spectrometric Identification Service
Peptide Post-Translational Modification Identification Service
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