Lactylation Proteomics Service
Lactylation Proteomics Service is a comprehensive mass spectrometry-based analytical solution that integrates enrichment strategies and bioinformatics analysis to identify, quantify, and functionally characterize protein lactylation in biological samples. This service enables the discovery of lactylation-regulated pathways, elucidates regulatory mechanisms at critical metabolic nodes, and supports research on disease mechanisms, target identification, and the development of novel therapeutic strategies.
Wang J. et al. Cell Mol Biol Lett. 2024.
Lactylation, a newly identified lysine post-translational modification, was first discovered on histones. It originates from the covalent attachment of lactate molecules to lysine residues and is closely linked to glycolysis and cellular metabolic states. Emerging research indicates that lactylation plays a crucial role in cellular metabolism, inflammatory responses, immune regulation, and tumor progression. This modification not only alters the charge and conformation of proteins but also affects protein activity, localization, and interactions—thereby influencing cell fate and disease mechanisms.
Leveraging advanced chromatography and mass spectrometry platforms, MtoZ Biolabs offers Lactylation Proteomics Service to accurately identify and quantify lactylated proteins and their modification sites, helping to deeply analyze the functional relationship between lactate metabolism and epigenetic regulation, and providing reliable proteomics solutions for research fields such as tumors, immunity, and metabolism.
Analysis Workflow
Cheng Z. et al. iScience. 2024.
The main workflow of Lactylation Proteomics Service is as follows:
1. Sample Preparation
Proteins are extracted and digested from the sample after quality assessment.
2. Lactylated Peptide Enrichment
Lactylated peptides are selectively enriched using anti-lactyl lysine antibodies.
3. LC-MS/MS Analysis
Enriched peptides are analyzed using nano-liquid chromatography coupled with high-resolution tandem mass spectrometry.
4. Data Processing and Quantification
Identified peptides are searched against protein databases to determine modification sites and perform quantitative analysis.
5. Bioinformatics Analysis
Functional annotation, pathway enrichment, and protein–protein interaction analyses are conducted for lactylated proteins.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Lactylation Proteomics Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Lactylation Proteomics data, providing clients with a comprehensive data report.
4. High-specificity Enrichment Strategy: Adopt optimized immune enrichment technology to effectively improve the detection rate of lactylated modified peptides.
5. One-stop Research Solution: From sample pretreatment, lactylated peptide enrichment, mass spectrometry analysis to data interpretation, the whole process is controlled by a professional team to ensure stable and reliable results.
Sample Submission Suggestions
MtoZ Biolabs accepts a wide range of sample types, including but not limited to:
To maintain sample integrity, repeated freeze-thaw cycles should be avoided. It is recommended to store samples at -80°C and ship them on dry ice.
You are welcome to communicate your specific needs with the MtoZ Biolabs technical team before submitting your samples. We can provide customized sample processing suggestions and pre-treatment services.
Applications
Specific application examples of Lactylation Proteomics Service:
Epigenetic Regulation Studies
Elucidate the role of lactylation in chromatin remodeling and gene expression regulation.
Disease Mechanism Investigation
Explore the involvement of lactylation in tumors, inflammation, hypoxia-related disorders, and its potential as a diagnostic or therapeutic target.
Immunoregulation Research
Study the impact of lactylation on immune cell activation and functional differentiation.
Signal Transduction Analysis
Identify key signaling proteins and regulatory axes modulated by lactylation, providing insights for signaling network modeling.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Post-Translational Modifications Proteomics Service
Deamidation Proteomics Service
Adenylylation Proteomics Service
How to order?
