High-Throughput Fucose Screening Service
Fucose is an important monosaccharide that is widely found on glycoproteins, glycolipids, and other glycan molecules. As part of glycosylation modifications, fucose plays a critical role in many biological processes. Fucose is involved in cell recognition, immune regulation, pathogen recognition, tumor progression, and glycan–protein interactions. Investigating the structure, distribution, and function of fucose is essential for understanding disease mechanisms, drug development, and immune evasion mechanisms.
Figure 1. The Structure of Fucose
MtoZ Biolabs offers a High-Throughput Fucose Screening Service to support efficient and accurate analysis of interactions between fucosylated glycans and biological targets. This service is widely applied in glycan–protein interaction studies, immune response analysis, pathogen recognition, and cancer biomarker screening.
Services at MtoZ Biolabs
The multi-platform high-throughput fucose screening service offered by MtoZ Biolabs combines with advanced automated experimental workflows to efficiently screen the interactions between fucose-related glycan molecules and biological targets. It includes but is not limited to the following main screening strategies:
Table 1. High-Throughput Fucose Screening Strategies at MtoZ Biolabs
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We immobilize a variety of fucose-modified glycan molecules on glass slides and react them with fluorescently labeled targets such as proteins, antibodies, or cell lysates. Using high-resolution scanning systems, we monitor and analyze the binding of the glycan molecules to the targets in real-time. This platform is ideal for large-scale screening and rapid evaluation of fucose-related glycan–protein interactions. |
Bead-Based Screening Fucose-modified glycan molecules are conjugated to magnetic or polymer beads, then incubated with receptor proteins, cells, or antibodies in liquid phase. Binding signals are detected using ELISA or flow cytometry (FACS). This method is suited for medium-throughput screening and is particularly useful for functional validation and initial screening. |
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Fluorescent Fucose Probe Assay We utilize fluorescently labeled fucose probes to assess the binding capacity of target molecules to fucose. This method is particularly useful for analyzing fucose–cell surface receptor interactions, providing insights into binding at the cellular level. |
Surface Plasmon Resonance (SPR) / Bio-Layer Interferometry (BLI) Based on SPR or BLI technology, this method allows for real-time monitoring of the interaction between fucose-modified glycans and target molecules without the need for labeling. It is suitable for analyzing binding affinity and kinetic parameters, providing high-sensitivity analysis of fucose–target interactions. |
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Mass Spectrometry-Based Screening Using MALDI-TOF or LC-MS/MS platforms, we perform qualitative and quantitative analysis of fucose molecules that bind during screening. This method, combined with automated data processing workflows, supports high-throughput data collection and structural analysis. |
Analysis Workflow
1. Project Communication and Plan Design
Define the research goals, design a customized screening plan, and select the appropriate technology pathway.
2. Sample Preparation and Quality Control
Prepare fucose-modified glycan molecules or target biomolecules, followed by quality control.
3. Screening Experiment Implementation
Perform high-throughput screening of fucose and biomolecular interactions on an automated platform.
4. Data Acquisition and Analysis
Collect experimental data, and analyze binding signals using standardized analytical methods.
5. Results Delivery and Technical Support
Provide a detailed screening report, interpret the results, and offer technical support.
Service Advantages
1. Multi-Platform Technology Collaboration
We integrate various screening technologies, including glycan microarrays, bead-based screening, flow cytometry, optical sensing, SPR/BLI, and mass spectrometry, to offer flexible and efficient solutions based on research needs.
2. High-Throughput Automated Platforms
Our automated experimental platforms significantly enhance experimental efficiency, ensuring standardization and reproducibility of results to meet large-scale screening demands.
3. Customized Screening Services
We offer a wide range of fucose-modified glycan libraries and can customize specific fucose structures for screening, catering to various research directions.
4. Comprehensive Research Continuum
In addition to high-throughput screening, we provide downstream services such as kinetic analysis, functional validation, and cell recognition studies, ensuring a complete research pathway.
5. Transparent Pricing
Our pricing is transparent, ensuring customers do not need to worry about additional fees or hidden costs.
Applications
1. Fucose-modified glycan interactions with receptor proteins
2. Fucose-based vaccine design and optimization
3. Fucose in cancer and metabolic disease biomarker screening
4. Exploring glycan–glycan interactions and identifying glycomimetic drug targets
5. Fucose-based antibody or receptor recognition analysis
FAQs
Q1: Can I customize the screening for specific fucose structures?
Yes, we support the customization of specific fucose structures for screening, either by providing our own probes or by using customer-provided structures to meet personalized needs.
Q2: How long does the high-throughput fucose screening service take?
Typically, it takes 2-3 weeks. If the screening involves customized structures or more complex targets, the process may take longer. The specific timeline will be determined based on the project's requirements.
Sample Submission Suggestions
1. Protein Samples: Recommended quantity ≥100 µg, purity >90%, lyophilized or low-salt liquid state
2. Antibodies/Cell Lysates: Please provide source, concentration, and storage conditions
3. Fucose Derivatives (Customer Provided): Please specify structure, purity, solvent, etc.
4. Other Special Samples: Contact us for technical advice on sample preparation
Deliverables
1. Documentation of screening platform and experimental workflow
2. Raw data files and processed data tables
3. Fucose-binding spectra, heatmaps, and other visual results
4. Statistical analysis report of screening data
5. Professional technical report, including method overview, experimental conditions, and result interpretations
Fucose plays a critical role in many biological processes. Investigating its function and interactions is essential for understanding disease mechanisms and developing new drugs. MtoZ Biolabs offers a high-throughput fucose screening service to help researchers identify fucose-related biomolecular interactions, advancing glycomics, drug discovery, and disease research.
Contact us today to learn more about your project needs. We provide customized experimental plans and professional technical services.
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