High-Throughput Mannose Screening Service

Mannose is a naturally occurring monosaccharide widely present in glycoproteins and glycolipids of animal cells. It plays a key role in N-linked glycan structures and serves as an important recognition motif in various biological processes, including pathogen recognition, immune regulation, and intercellular signaling. Studies have shown that many pathogens (such as viruses and bacteria) utilize mannose recognition to attach to and invade host cells. Furthermore, mannose has demonstrated valuable applications in vaccine design, drug targeting, and cancer glycomics research. High-throughput mannose screening is a powerful strategy that uses automated platforms and large-scale parallel detection to systematically evaluate interactions between mannose (or mannose-modified molecules) and proteins, antibodies, receptors, or cells. This technique enables rapid identification of high-affinity or specific binders, offering an efficient tool for both basic glycobiology research and applied discovery projects.

 



Figure 1. Mannose Plays a Key Role in N-linked Glycan Structure

 

As a professional high-throughput screening service provider, MtoZ Biolabs integrates multi-platform analytical capabilities with advanced automated systems to offer High-Throughput Mannose Screening Service for researchers and biotechnology companies, facilitating drug discovery, vaccine evaluation, and functional glycan analysis.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers a range of technical platforms to support high-throughput mannose screening service covering the following mainstream approaches:

 

Table 1. High-Throughput Mannose Screening Strategies at MtoZ Biolabs

Glycan Microarray We present a diverse library of mannose-containing glycans on array platforms for parallel screening with fluorescent-labeled biological targets. This allows for efficient identification of mannose-specific interactions, including pathogen-binding motifs or immune recognition elements.	Bead-Based Screening Mannose glycans are conjugated to microspheres or magnetic beads and incubated with proteins, cells, or antibodies. Binding events are quantified via ELISA or flow cytometry, making this method ideal for functional assays and initial binding validation.
Fluorescent Mannose Probe Assay We use fluorescent-labeled mannose probes to assess interactions at the cell surface level. This approach is especially relevant for studying mannose receptor binding, immune lectin engagement, or pathogen adhesion studies.	Surface Plasmon Resonance (SPR) / Bio-Layer Interferometry (BLI) SPR/BLI provides kinetic characterization of mannose–target binding without the need for labeling. It enables quantitative measurement of association/dissociation rates, offering insight into molecular recognition mechanisms.
Mass Spectrometry-Based Screening MALDI-TOF or LC-MS/MS techniques are applied to identify and quantify mannose-bound molecules post-screening. These tools allow high-throughput structural analysis and support downstream bioinformatic interpretation.

 

Analysis Workflow

1. Project Consultation and Experimental Design: Define research objectives and recommend suitable screening platforms

2. Sample Preparation and QC: Prepare mannose probes or candidate molecules based on project needs

3. High-Throughput Assay Execution: Perform screening using automated platforms

4. Data Acquisition and Analysis: Process and evaluate data using standardized pipelines

5. Results Delivery and Technical Support: Provide comprehensive reports and interpretation guidance

 

Service Advantages

1. Integrated Multi-Platform Capabilities

We combine microarray, flow cytometry, mass spectrometry, and optical biosensor technologies. Based on your project goals, we select the most appropriate platform to ensure accurate, efficient screening.

 

2. High-Throughput Automation

All platforms are built on automated systems capable of processing hundreds to thousands of samples or structural variants, significantly shortening turnaround times while ensuring reproducibility.

 

3. Comprehensive Mannose Structure Library

We maintain a diverse library of mannose structures with various linkages and conformations. Custom probe synthesis is also available to enhance screening specificity and translational value.

 

4. Support for Downstream Validation

In addition to primary screening, we offer follow-up services including binding kinetics assays, cellular validation, and receptor identification, enabling end-to-end support from discovery to functional analysis.

 

5. Transparent Pricing

Our pricing is transparent, with no hidden fees or additional costs.

 

Applications

1. Screening and affinity analysis of mannose-binding proteins

2. Evaluation and optimization of mannose-based vaccine candidates

3. Target identification and mechanism of action studies for glycan-based therapeutics

4. Receptor profiling in mannose-related immune recognition pathways

5. Functional screening of mannose-modified glycans

 

FAQs

Q1: Can the service provide quantitative data?

Yes. Our microarray, flow cytometry, and MS-based platforms support semi-quantitative or quantitative binding analyses suitable for downstream validation.

 

Q2: How long does the high-throughput mannose screening service take?

Typical turnaround time is 2–3 weeks after receiving samples. For customized structures or complex assays, timelines may vary.

 

Q3: Are the results suitable for publication or patent filing?

Yes. All data and figures meet research standards and can support peer-reviewed publications and intellectual property applications.

 

Sample Submission Suggestions

1. Protein samples: ≥100 µg, >90% purity, provided as lyophilized or low-salt solutions

2. Antibodies/Cell lysates: Please specify source and concentration

3. Mannose structures: For custom probes, provide linkage information, purity, and solvent details

4. Other sample types: Please consult us in advance

5. A detailed sample submission guideline is available from MtoZ Biolabs to ensure consistency and data reliability.

 

Deliverables

1. Description of screening platform and experimental procedures

2. Raw data and processed result tables

3. Binding heatmaps or profiles of mannose-target interactions

4. Statistical analysis including affinity ranking and significance testing

5. Comprehensive technical report with experimental parameters and data interpretation

 

High-throughput mannose screening is a powerful tool in glycobiology and translational research, particularly for identifying mannose-binding proteins and developing glycan-targeted drugs and vaccines. MtoZ Biolabs is committed to delivering reliable, efficient, and customizable high-throughput mannose screening service to accelerate your scientific progress.

 

If you are interested in our service, please don’t hesitate to contact us for a tailored proposal and quotation. We look forward to being your trusted partner in glycomics research.

 

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