High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis Service

High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis Service utilizes automated micro-permethylation combined with mass spectrometry to achieve high-throughput and accurate structural characterization of complex glycans, providing efficient and reliable solutions for glycomics research, disease mechanism exploration, and drug development.

Glycans, as essential modifications on cell surfaces and secreted proteins, play critical roles in signal transduction, immune regulation, pathogen recognition, and disease progression. Due to the high complexity and heterogeneity of glycan structures, their analysis has long been a central challenge in glycomics research. Traditional glycan analysis relies on labor-intensive manual procedures, which are not only inefficient but also prone to human error. Glycan structure analysis based on High-Throughput Automated Micro-Permethylation combined with mass spectrometry involves automated micro-permethylation of glycan samples followed by electrospray ionization mass spectrometry (ESI-MS) or matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). This approach enables rapid, accurate, and systematic characterization of monosaccharide composition, branching patterns, and modification features of glycans. It significantly improves analytical efficiency and data reliability, while making large-scale glycomics studies feasible.

Services at MtoZ Biolabs

Leveraging advanced automated micro-methylation and mass spectrometry platforms, MtoZ Biolabs provides a High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis Service that can rapidly and accurately analyze the composition and structural characteristics of complex sugar chains under high-throughput conditions, providing reliable data support and systematic solutions for glycomics research, drug development, and immunology research.

Analysis Workflow

General workflow of High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis Service:

1. Sample Preparation

Optimize sample conditions to fit automated micro-permethylation reactions.

2. Automated Micro-Permethylation

Perform hydroxyl group methylation of glycans using a 96-well automated system.

3. Mass Spectrometry Detection

Select ESI-MS or MALDI-MS for high-resolution detection based on glycan molecular weight and research requirements.

4. Data Analysis

Obtain structural information including monosaccharide composition, linkage types, and branching patterns.

5. Result Reporting

Generate a comprehensive structural analysis report and visualized charts to facilitate downstream research.

high-throughput-automated-micro-permethylation-based-glycan-structure-analysis-service1.jpg

Shajahan A. et al. SLAS Technol. 2020.

Figure 1. High-Throughput and Microscale Analysis of N- and O-Linked Glycans through Micropermethylation and MALDI-MS/ESI-MS.

Service Advantages

Advanced Analysis Platform: MtoZ Biolabs established an advanced High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

High Throughput and Efficiency: The automated system enables simultaneous processing of dozens to hundreds of samples, significantly reducing analysis time.

Reduced Human Error: Automated operation minimizes manual intervention, ensuring higher reproducibility and stability of results.

Systematic Structural Analysis: Provides in-depth structural information by comprehensively revealing glycan monosaccharide composition, linkage types, and branching patterns.

High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all High-Throughput Automated Micro-Permethylation Based Glycan Structure Analysis data, providing clients with a comprehensive data report.

Sample Submission Suggestions

Sample Types

Accepts a wide range of sample types, including purified glycans, glycoproteins, and glycoconjugates.

Storage and Transportation

Store at low temperature (−20 °C or below). Shipping on dry ice is recommended to maintain sample stability.

It is recommended to contact the MtoZ Biolabs technical team prior to sample submission to obtain detailed and tailored guidelines for sample preparation and submission.

Applications

Glycomics Research: Enable large-scale structural analysis of glycan diversity and uncover their functional roles in cellular processes.

Drug Development: Support the design and quality control of complex glycosylated therapeutics.

Vaccine Development: Characterize glycan antigens relevant to immunity to facilitate the design and optimization of glycan-based and glycoconjugate vaccines.

Molecular Recognition Studies: Investigate how glycans regulate signaling pathways and mediate cellular interactions.

FAQ

Q1: Is the Automated Micro-Permethylation Method Applicable to All Types of Glycans?

A1: Yes. This method is broadly applicable to various glycan types, including N-glycans, O-glycans, and glycolipids. However, for certain complex or heavily modified glycans (such as highly sialylated structures), we adjust the reaction conditions according to sample characteristics to ensure optimal results.

Q2: What Is the Detection Limit? How Does It Perform With Low-Abundance Glycans?

A2: The platform at MtoZ Biolabs achieves detection sensitivity at the picomole (pmol) level and provides strong analytical performance for low-abundance glycans. In addition, automated processing minimizes sample loss and enhances the detection rate of rare components.