Analytical Ultracentrifugation Molecular Weight Determination Service

Analytical ultracentrifugation molecular weight determination service is a molecular weight measurement service based on analytical ultracentrifugation, which observes the sedimentation behavior of molecules in solution under high centrifugal force to directly determine their molecular weight and size distribution. This method requires no labeling or chemical modification, allowing for accurate analysis of samples in their true state, and is widely used for proteins, nucleic acids, and complex biomolecules. By employing analytical ultracentrifugation for molecular weight determination, intrinsic information about the sample in solution can be obtained, including molecular weight, shape, and aggregation state, which is particularly valuable for studying molecular interactions, determining aggregation ratios, and assessing sample purity. This service provides essential data support and structural confirmation for high-precision analysis, making it a valuable tool for research and biopharmaceutical development that require a detailed understanding of sample structural characteristics and behavior in solution.

 

Service at MtoZ Biolabs

MtoZ Biolabs offers an analytical ultracentrifugation molecular weight determination service utilizing the most advanced analytical ultracentrifugation (AUC) technology. This is a universal, rigorous, and accurate method for determining the molecular weight, hydrodynamic, and thermodynamic properties of proteins and other macromolecules. As an independent, first-principles-based technique, AUC requires no standards and performs measurements in a matrix-free environment, ensuring highly reliable and precise results. Although AUC has a history of nearly a century, it remains unparalleled in terms of information range, precision, and accuracy, and is hailed as the gold standard for in-solution protein molecular weight determination. MtoZ Biolabs possesses advanced instrumentation and a professional technical team capable of directly analyzing the true state of samples, providing customers with comprehensive molecular characteristic analysis and precise data support, contributing to the success of scientific research and biopharmaceutical development. Our service requires no labeling or chemical modification, directly determining the molecular weight and structural information of biomolecules, providing the most authentic and reliable foundation for your research. If you are interested in our services, please feel free to contact us.

 

Service Advantages

1. Professional Data Analysis and Interpretation Expertise: MtoZ Biolabs has an experienced team of scientists proficient in advanced mathematical models and data fitting methods, capable of accurately interpreting the complex raw data generated by AUC. We ensure precise measurement of key parameters such as molecular weight, conformation, and interactions, providing reliable data support for your research.

 

2. Rigorous Sample Handling and Experimental Condition Control: We place great emphasis on sample purity and stability, utilizing optimized sample preparation procedures to minimize the impact of impurities, aggregates, or degradation products. Additionally, MtoZ Biolabs strictly controls experimental conditions like temperature, centrifugal force, and buffer composition to ensure high reproducibility and accuracy of results.

 

3. Advanced Equipment and Professional Team Assurance: MtoZ Biolabs is equipped with state-of-the-art analytical ultracentrifugation instruments featuring high sensitivity and high resolution. Our professional technicians are thoroughly trained and skilled in operating AUC equipment, enabling efficient experiment completion and accurate result interpretation, saving you time and resources while ensuring smooth project progression.

 

4. Customized Solutions and Efficient Services: MtoZ Biolabs is committed to meeting the unique needs of our clients by providing tailored analysis schemes, ensuring that results precisely align with your research objectives. Meanwhile, our efficient service processes significantly shorten project timelines, assisting your research and development progress.

 

Sample Submission Suggestions

1. Sample Types: We accept proteins, polysaccharides, or complex biomolecules, including monomers, dimers, or molecules in higher aggregated forms.

 

2. Temperature Control: Select an appropriate storage temperature based on the sample's characteristics-such as 4 °C, -20 °C, or -80 °C-to maintain sample stability.

 

3. Sample Storage: Avoid using components that may interfere with the experiment, such as high salt concentrations, strong acids or bases, detergents, metal ion chelators, or organic solvents. Common buffers include PBS (phosphate-buffered saline), Tris-HCl buffer, etc., and the pH should be suitable for the sample's stability requirements.

 

Case Study

1. Molecular weight distribution analysis by ultracentrifugation: Adaptation of a new approach for mucins

 



Gillis, RB, et al. Carbohydr Polym. 2013.

 

2. Molecular Weight Distribution Evaluation of  Polysaccharides and Glycoconjugates Using  Analytical Ultracentrifugation

 



Harding, SE. et al. Macromol Biosci. 2010.

 

FAQ

1. In long-duration experiments, how can we strictly control experimental conditions to ensure the reproducibility of results?

We should ensure the stability of the laboratory environment, avoiding fluctuations in temperature and humidity. Use high-precision temperature control equipment and calibrate it regularly to maintain consistent temperatures. The stability of centrifugal force also requires attention; regularly check the mechanical performance of the instrument. The preparation of buffer solutions must be precise—use fresh reagents and strictly control pH values and ionic strength. To minimize variations in conditions, it's recommended to develop a detailed experimental protocol, follow the steps meticulously, and record all experimental parameters. By implementing these measures, we can enhance the stability of the experiment and ensure the reproducibility of the results.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Molecular Weight Determination 

5. Mass Spectrometry Image

6. Raw Data