What Molecular Weight Cut-Off Should Be Chosen for Dialysis Bags in Bacterial Polysaccharide Purification?
When performing dialysis purification of bacterial polysaccharides, the molecular weight cut-off (MWCO) of the dialysis bag should be smaller than the polysaccharide’s molecular weight. It is generally recommended to select a MWCO that is one-third to one-half of the polysaccharide's molecular weight. For instance, if the molecular weight of the bacterial polysaccharide is approximately 300,000 Da, the MWCO of the dialysis bag should be between 100,000 and 150,000 Da. This ensures that small molecular impurities, such as salts and low-molecular-weight proteins, can pass through the dialysis membrane, while the polysaccharide is effectively retained.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Submit Inquiry
How to order?
