What Is the Best Method for Metabolite Detection
Metabolite detection is commonly performed using the following methods:
1. Mass Spectrometry (MS)
Mass spectrometry enables accurate determination of both the mass and structural features of metabolites. Commonly used techniques include Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS).
2. Nuclear Magnetic Resonance (NMR)
NMR provides detailed structural information about metabolites and is particularly suitable for analyzing complex biological samples without requiring extensive sample pretreatment.
3. High-Performance Liquid Chromatography (HPLC)
HPLC is widely applied for the separation and quantification of a broad range of metabolites. It is typically used in conjunction with detectors such as ultraviolet (UV) or fluorescence detectors. However, its sensitivity is generally lower compared to mass spectrometry-based methods.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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