Ubiquitinated Peptide Site Identification Service

MtoZ Biolabs provides ubiquitinated peptide site identification service using high-resolution mass spectrometry platforms and specific enrichment strategies to help academic and industrial clients accurately identify ubiquitination modification sites in proteins, thereby revealing key mechanisms such as protein degradation, signal transduction, and cellular homeostasis regulation. This service is applicable to cell, tissue, and recombinant protein samples, providing high-quality data support and systematic solutions for post-translational modification studies.  

 

What is Ubiquitination  

Ubiquitination is an important form of post-translational modification in which a small 76-amino-acid protein called ubiquitin is covalently attached to lysine residues of target proteins. This process regulates protein stability, localization, activity, and interactions.  

 



Gong, X. et al. Invest New Drugs. 2020.

Figure 1. The Process of Ubiquitination

 

This modification plays a central role in various cellular processes including protein degradation, DNA repair, signal transduction, and immune responses. Abnormal ubiquitination is often associated with cancer, autoimmune diseases, and neurodegenerative disorders. Therefore, accurate identification of ubiquitination sites is essential for understanding protein function and disease mechanisms.  

 

Ubiquitinated Peptide Site Identification Service at MtoZ Biolabs  

The ubiquitinated peptide site identification service provided by MtoZ Biolabs integrates immunoaffinity enrichment, chemical derivatization, and high-resolution LC-MS/MS analysis to systematically detect and map ubiquitinated peptides.  

 

Our service covers the entire workflow from sample preparation, enrichment, and mass spectrometry detection to bioinformatics analysis, enabling the identification of mono-, multi-, and poly-ubiquitination modifications.  

 

The analysis results help researchers determine modification sites, characterize modification patterns, and explore their biological roles in cellular processes.  

 

Workflow of Ubiquitinated Peptide Site Identification Service  



Why Choose MtoZ Biolabs  

Choosing MtoZ Biolabs means choosing a stable and reliable research partner.  

☑️ Multi-platform support: Equipped with multiple mass spectrometers such as Orbitrap Fusion Lumos and Q Exactive HF to meet different sensitivity requirements.  

☑️ Professional enrichment strategies: Offering both antibody-based and chemical capture methods to suit diverse sample types.  

☑️ Experienced team: Extensive expertise in post-translational modification research and complex sample analysis.  

☑️ One-Time Charge: Our pricing is transparent, with no hidden fees or additional costs.  

 

Applications of Ubiquitinated Peptide Site Identification Service   

🔹 Protein degradation pathway studies: Elucidating the regulation of the ubiquitin-proteasome system.  

🔹 Signal transduction analysis: Exploring how ubiquitination influences cellular responses.  

🔹 Disease mechanism studies: Identifying ubiquitination sites associated with cancer and neurodegenerative diseases.  

🔹 Drug target discovery: Assisting in the identification of ubiquitin-regulated therapeutic targets.  

🔹 Protein modification interaction studies: Integrating with phosphorylation and acetylation data for cross-regulation analysis.  

 

FAQs  

Q1: What is the service's general workflow?  

 

Q2: What data formats are provided?  

MtoZ Biolabs provides multiple standardized result formats to ensure compatibility with common analysis and visualization software. Typical deliverables include  

• Raw data files generated by LC-MS/MS platforms  

• Processed identification and quantification result tables in CSV or Excel format  

• Comprehensive analysis reports in PDF format including analytical methods, quality control, and key conclusions  

• High-resolution spectra and chromatograms in TIFF or PNG format  

Additional formats can be provided upon request to meet specific research or publication needs.  

 

Q3: How should I prepare my samples?  

To obtain accurate and reproducible ubiquitinated peptide analysis results, please follow the sample preparation guidelines below  

1. Sample type: Cell lysates, tissue samples, serum, plasma, and purified proteins are all accepted.  

2. Sample purity: Avoid high concentrations of salts, detergents, or organic solvents that may interfere with digestion or ionization efficiency.  

3. Sample amount: For liquid samples, provide at least 500 µg total protein. For tissue samples, at least 20 mg is recommended.  

4. Storage and shipping: All samples should be stored at −80°C and shipped on dry ice to maintain stability.  

5. Sample information: Include details such as sample source, preparation method, buffer composition, and desired analysis objectives.  

 

For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics

 

If you are unsure about your sample preparation, our technical team can provide personalized pre-analysis consultation and a sample submission guide to help you optimize preparation and shipping conditions.

Start Your Project with MtoZ Biolabs  

Through the ubiquitinated peptide site identification service, MtoZ Biolabs helps researchers uncover the dynamic regulatory mechanisms of protein ubiquitination, providing accurate and reliable data support for disease research, drug development, and signal transduction studies. Contact us to obtain customized solutions tailored to your research needs.