Sucrose Analysis Service

As a disaccharide composed of glucose and fructose linked through a glycosidic bond, sucrose serves as the primary transport sugar in plants and a key energy source for numerous organisms. Beyond its biological role, sucrose plays a pivotal part in food manufacturing, pharmaceuticals, and bioprocessing due to its sweetness, stability, and physicochemical versatility. Accurate analysis of sucrose content and structural integrity is therefore vital for quality control, nutritional assessment, and biochemical research.

To meet the growing analytical demands across industries, MtoZ Biolabs provides a comprehensive Sucrose Analysis Service designed to support researchers and manufacturers in characterizing sucrose with precision and reliability. Our service covers multiple analytical dimensions, including sucrose content determination, purity evaluation, structural characterization, stability testing, degradation profiling, and metabolic pathway analysis. Equipped with state-of-the-art analytical platforms and expert data interpretation, MtoZ Biolabs offers end-to-end sucrose testing solutions tailored to academic, industrial, and regulatory applications.

Service at MtoZ Biolabs

💠Sucrose Content Determination: Quantitative analysis of sucrose concentration in plant tissues, food products, pharmaceuticals, and biological samples using HPLC, LC-MS, or enzymatic assays.

💠Sucrose Purity Analysis: Evaluation of sucrose purity and detection of contaminants such as glucose, fructose, maltose, and other carbohydrates to ensure product quality.

💠Sucrose Structural Characterization: Determination of sucrose's molecular structure and confirmation of glycosidic linkages through NMR, spectroscopic, mass spectrometric, and other advanced methods.

💠Sucrose Stability Assessment: Monitoring of sucrose chemical stability under different environmental or formulation conditions such as pH, temperature, and humidity.

💠Sucrose Degradation Studies: Investigation of hydrolysis and caramelization processes to identify degradation products and understand reaction kinetics.

💠Sucrose Metabolic Pathway Analysis: Tracing sucrose metabolism and conversion pathways in plants, microorganisms, or model systems through isotopic labeling and metabolomic profiling.



Correia, D. M. et al. Front Nutr. 2014.

Figure 1. Typical Chromatogram Recorded for a Standard Solution (A) and a Fermentation Sample (B) Containing Sucrose and Other Carbohydrates

Technical Platforms at MtoZ Biolabs

🔸High-Performance Liquid Chromatography (HPLC): A widely used method for sucrose quantification and purity analysis, utilizing refractive index or evaporative light scattering detectors for accurate measurement.

🔸Liquid Chromatography-Mass Spectrometry (LC-MS): Enables simultaneous detection and structural verification of sucrose and its related saccharides with high sensitivity.

🔸Gas Chromatography (GC): After derivatization, GC provides excellent separation and detection of sucrose and degradation products.

🔸Infrared (IR) and Nuclear Magnetic Resonance (NMR) Spectroscopy: Used for confirming glycosidic bonds, molecular structure, and conformational changes.

🔸Enzymatic Assays: Offer specific and efficient quantification by catalyzing sucrose hydrolysis through invertase or related enzymes, with measurement of resultant glucose and fructose.

Analysis Workflow



Service Advantages

☑️Advanced chromatographic and spectroscopic instrumentation ensuring high-resolution separation and detection.

☑️Capability to analyze a wide range of sample matrices, including solids, liquids, biological extracts, and complex mixtures.

☑️Experienced analytical chemists specializing in carbohydrate chemistry and mass spectrometry.

☑️Integrated approach combining multiple analytical methods for complete sucrose profiling.

☑️Flexible experimental design tailored to both academic research and industrial testing requirements.

☑️Rapid turnaround time and full confidentiality in data handling and reporting.

Applications

1. Food Industry

Quantification of sucrose in beverages, confectionery, and processed foods for nutritional labeling and quality control.

2. Agricultural Research

Analysis of sucrose content and metabolism in crops to evaluate photosynthetic efficiency and carbohydrate allocation.

3. Pharmaceutical Development

Assessment of sucrose as an excipient in formulations to monitor stability, purity, and degradation behavior.

4. Fermentation and Biotechnology

Monitoring sucrose consumption and conversion efficiency in microbial fermentation systems.

5. Metabolomics and Systems Biology

Study of sucrose dynamics in metabolic pathways and stress responses in plants or microbes.

6. Material Science

Investigation of sucrose-derived carbon precursors in biopolymer and carbon material synthesis.

Sample Submission Suggestions



*Note: For other special sample types or low-yield materials, please contact us before preparation.

Deliverables

• Detailed experimental conditions including analytical methods, instruments, and calibration parameters.

• Raw and processed data files with chromatograms, spectra, or quantitative tables.

• Graphical representation of sucrose concentration, purity, and structural features.

• Comprehensive analytical report with data interpretation and recommendations.

Ready to start your project?

Contact our scientific support team to discuss your experimental design or request a quote.