Resources

Proteomics Databases



Metabolomics Databases

• • Determining Protein Content in E. coli via Amino Acid Analysis

  In the field of microbiology and biotechnology, Escherichia coli, as an important model organism and industrial production microbe, accurate measurement of its protein content is of great significance for basic research and industrial applications. MtoZ Biolabs provides efficient and reliable technical means for the precise determination of protein content in Escherichia coli using advanced amino acid analysis.

• • Identifying Protein Phosphorylation Sites via Mass Spectrometry

  In mass spectrometry analysis, proteins or peptide segments are ionized into charged particles and then separated through a mass spectrometer. The mass spectrum displays the relationship between the mass-to-charge ratio (m/z) of these particles and their intensity (signal size). Interpreting the mass spectrum requires understanding the fragmentation patterns of peptide segments and the mass changes caused by phosphorylation.

• • Steps for Protein Determination Using Circular Dichroism

  Circular Dichroism (CD) is a spectroscopic technique based on electromagnetic waves that can analyze the structural features of proteins by measuring the difference in absorption of left and right circularly polarized light. It is currently widely used in determining the secondary structure of proteins, and is a fast, simple, and relatively accurate method for studying the conformation of proteins in dilute solutions.

• • Bradford Protein Concentration

  Bradford method, also known as the Coomassie Brilliant Blue method, is a fast and simple method for determining protein concentration. The method is based on the binding of proteins with a dye (Coomassie Brilliant Blue G-250), and protein concentration is determined by colorimetry.

• • How Much Protein Is Needed for Mass Spectrometry Sequencing?

  The amount of protein needed for mass spectrometry determination of protein sequence depends on several factors, including the sensitivity of the mass spectrometer, the purity of the sample, the type of analysis (such as peptide mapping, protein identification or post-translational modification analysis) and the properties of the specific protein. With advances in technology, the sensitivity of mass spectrometry is increasing and the amount of sample required is decreasing.

• • Protein Sequence Homology Analysis

  Protein sequence homology analysis is an important technique in bioinformatics, used to identify the similarity and evolutionary relationship between two or more protein sequences. Homology analysis is crucial for predicting the function of unknown proteins, studying the evolutionary history of proteins, and discovering new biological markers.

• • What Analysis Can Be Performed on Protein Sequencing Results?

  After obtaining the protein sequencing results, various analyses can be performed. These analyses help to reveal the function, structure, interactions and roles of the protein in the organism. The commonly used types of analysis mainly include:   1. Sequence Alignment Align with known protein sequences to identify homologous proteins, which helps to infer protein function and evolutionary relationships.   2. Functional Domain and Active Site Prediction Identify functional domains and active sites in protein

• • Can Protein Mass Spec Analysis Determine Amino Acid Aequences?

  Protein mass spectrometry analysis: An effective tool for obtaining amino acid sequences of proteins. Proteins are one of the most important functional molecules in living organisms, performing functions such as catalyzing biochemical reactions, constructing cellular structures, and regulating biological activities. Therefore, understanding the relationship between protein structure and function is crucial for biological and medical research.

• • Determination of Collagen Molecular Weight

  Collagen molecular weight determination is a technique used to determine the molecular weight of collagen, a complex protein. Collagen is one of the most abundant proteins in animals and is mainly found in the skin, bones, tendons, and connective tissues. Accurate determination of collagen molecular weight is of great significance in biomedical and cosmetic industries due to its critical role in cellular structure and tissue regeneration.

• • Application of TMT Labeling in Quantitative Proteomics Detection

  TMT (Tandem Mass Tag) labeling-based quantitative proteomics is an advanced mass spectrometry technique used to analyze and quantify protein expression differences in different samples. The core of this technique involves labeling protein samples with TMT tags. During this process, protein samples from different conditions or treatments are individually labeled with different TMT tags.