Resources
Proteomics Databases
Metabolomics Databases
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• Sixplex TMT 10-Level Proteomics
TMT (Tandem Mass Tags) technology is a widely utilized method for quantitative proteomics, enabling the simultaneous comparison of protein expression levels across multiple samples. In a 10-plex TMT experiment, up to ten different samples can be quantitatively compared. Each TMT tag corresponds to a distinct mass, which can be accurately identified using a mass spectrometer. These tags are covalently attached to proteins or peptides, allowing samples from varying conditions or time points to be analyzed....
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• PRM-Targeted Proteomics Technology
Targeted proteomics, especially Parallel Reaction Monitoring (PRM), is a highly precise mass spectrometry method used for quantifying specific protein expression levels. In contrast to traditional proteomics methods, PRM allows for more precise quantification of specific peptides and proteins by precisely controlling mass spectrometer conditions, thereby increasing selectivity and sensitivity.
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Chemical proteomics is an emerging interdisciplinary field that integrates chemical techniques with proteomic analysis, aiming to selectively label, separate, and identify specific proteins in complex biological systems using chemical methods. This technology can reveal the structure and function of proteins within living organisms and has significant potential in drug discovery, disease diagnosis and treatment, as well as basic biological research. Through chemical proteomics, scientists can more eff......
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Cataract is a prevalent ophthalmic disorder caused by abnormal aggregation of lens proteins, leading to lens opacification, and remains one of the leading causes of blindness worldwide. The rapid advancement of the digital age has underscored the importance of eye health. Current treatment primarily involves surgical removal of the opaque lens, but the high costs and risks associated with surgery emphasize the need for non-surgical therapeutic approaches. Recently, the Ophthalmology Center of the Seco......
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• iTRAQ/TMT Quantitative Proteomics Steps
Sample Preparation 1. Protein Extraction: Total proteins are extracted from various biological samples. 2. Protein Quantification: Protein concentrations are determined using methods such as BCA or Bradford assays. Protein Digestion: Proteins are enzymatically digested using trypsin or other enzymes, cleaving at specific residues like lysine or arginine, to generate peptides.
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• Can the Binding of Small Molecules to Proteins Be Detected by LC-MS/MS
LC-MS/MS is a powerful analytical technique widely used for the qualitative and quantitative analysis of small molecules, peptides, proteins, and other biomacromolecules. In the study of small molecule-protein interactions, LC-MS/MS is an essential and irreplaceable technology.
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• How Many Proteins Can Label-Free Detect
Label-free technology is extensively applied in proteomics research for the identification and quantification of proteins within samples. Unlike methods that utilize fluorescent or radioactive labels, this approach employs mass spectrometry (MS) for the direct analysis of proteins. The number of detectable proteins is contingent upon several factors, such as the performance of the mass spectrometer, sample complexity, experimental design, and data processing and analysis techniques. Modern MS technologies..
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• Tobacco Co-Immunoprecipitation Experiment
Co-Immunoprecipitation (Co-IP) experiments in Nicotiana tabacum are employed to investigate protein-protein interactions. This method facilitates the identification and verification of interactions between specific proteins and their potential partners. Below are the detailed steps to perform a tobacco Co-IP experiment:
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• Mass Spectrometry Detects Molecular Weight
Mass spectrometry is an analytical technique that can accurately measure the masses of molecules and atoms. This is primarily done by measuring the mass-to-charge ratio (m/z) of ions. Mass spectrometry is also employed for the structural determination of compounds, for example, by measuring the masses of molecular ions and fragment ions.
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Since antigens were first successfully used to stimulate the immune system to produce antibodies, scientists have achieved significant breakthroughs in antigen research. Recently, a novel discovery of antigens has not only deepened our understanding of antigens and immune responses but also holds promising implications for vaccine development and immunotherapy.
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