Proteomics Commonly Used Mass Spectrometry
Proteomics is a field of study focused on understanding the structure and function of the entire proteome. This encompasses various aspects such as protein expression, post-translational modifications, interactions, and functional roles. The primary technique employed in proteomics research is mass spectrometry, a method capable of analyzing the molecular mass, structural characteristics, and chemical modifications of proteins.
Liquid Chromatography-Mass Spectrometry (LC-MS)
Liquid Chromatography-Mass Spectrometry (LC-MS) is a widely used technology in protein mass spectrometry. This technique involves separating proteins using liquid chromatography and subsequently determining their mass and chemical modifications through mass spectrometry. LC-MS is highly sensitive and accurate, enabling detailed analysis of complex protein samples.
Tandem Mass Spectrometry (MS/MS)
Tandem Mass Spectrometry (MS/MS) is a technique known for its high sensitivity in mass spectrometry. By conducting two rounds of mass spectrometry analysis, it provides valuable sequence and modification information of proteins. MS/MS is extensively utilized for both protein identification and quantification.
Label-Based Quantitative Techniques (iTRAQ and TMT)
Label-based quantitative techniques are employed for protein quantification. In these methods, proteins or peptides are labeled, and their labeling intensities across different samples are compared using mass spectrometry to quantify the proteins. iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and TMT (Tandem Mass Tag) are prominent methods in this category.
Label-Based Quantitative Techniques (SILAC and DM)
Label-based quantitative techniques, such as SILAC and DM, also focus on protein quantification. These methods involve the labeling of proteins or peptides, followed by mass spectrometry to compare labeling intensities among samples, thus enabling quantification. SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and DM (Dimethyl Labeling) are widely used techniques in this domain.
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