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    Resources

      Proteomics Databases

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      Metabolomics Databases

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    • • Protein Conservation Analysis

      Protein conservation analysis is a bioinformatics approach aimed at examining the similarities and differences in protein sequences across various organisms. At its core, this method involves comparing amino acid sequences to pinpoint regions that have remained unchanged throughout evolution and those that have undergone alterations. Evolutionarily conserved regions are often tightly linked to the structural integrity and biological functions of proteins, making them critical subjects for investigatio......

    • • BioID Proximity Labeling

      BioID proximity labeling is a powerful bioinformatics tool designed to investigate intracellular protein interactions and protein-protein interaction networks. This method involves tagging target proteins with a low-molecular-weight biotin-labeling enzyme, such as BirA, which catalyzes biotinylation in the presence of biotin. During this process, biotin is covalently attached to proteins located in close proximity to the target protein. These biotinylated proteins can then be isolated through affinity......

    • • Determining Amino Acid Sequence

      Determining amino acid sequence is a critical technique used to elucidate the arrangement of amino acids within a protein. Proteins are essential molecules that carry out a wide range of functions in living organisms. They consist of amino acids linked by peptide bonds, and the specific amino acid sequence dictates both the three-dimensional structure and biological function of the protein. This technique enables researchers to gain detailed insights into the functional mechanisms of proteins, particu......

    • • Protein Biomarker Discovery

      Protein biomarker discovery aims to provide vital tools for disease diagnosis, treatment, and prognosis by identifying and validating changes in protein profiles under specific biological conditions. Protein biomarkers are molecular indicators in organisms that reflect physiological or pathological states. These biomarkers are invaluable in biomedical research as they facilitate early disease detection, disease progression assessment, treatment monitoring, and the development of novel therapeutic stra......

    • • Protein Ligand Interaction Profile

      Protein ligand interaction profile is a critical approach for studying the relationships between proteins and their interacting molecules in biological systems. This analysis is focused on understanding the interaction processes between proteins and their ligands, which can include small molecules, ions, and biomacromolecules such as drug compounds, metabolites, and nucleic acids. These interactions are crucial in regulating biological processes, including signal transduction, metabolic control, and g......

    • • LC-MS Amino Acid Analysis

      LC-MS amino acid analysis is a powerful analytical method that integrates liquid chromatography (LC) and mass spectrometry (MS) to accurately identify and quantify amino acids in samples. The technique combines LC's ability to separate complex sample components with MS's capability to detect and quantify these components, resulting in high sensitivity, resolution, and versatility. This makes LC-MS amino acid analysis especially effective for examining complex samples with low amino acid concentrations......

    • • Western Blotting Quantitative

      Western Blotting Quantitative is a widely used technique for detecting and quantifying the expression levels of specific proteins in complex biological samples. This method integrates electrophoretic separation, membrane transfer, and antibody-based detection to deliver precise quantitative insights into target protein expression. The standard workflow involves separating proteins via polyacrylamide gel electrophoresis, transferring them onto nitrocellulose or polyvinylidene fluoride (PVDF) membranes,......

    • • Data-Independent Acquisition

      Data-Independent Acquisition (DIA) is a valuable technique for quantitative proteomics analysis in complex biological samples. Unlike traditional Data Dependent Acquisition (DDA), which selectively fragments specific precursor ions and may overlook low-abundance proteins, Data-Independent Acquisition simultaneously captures all precursor ions and their fragments in a single experiment. This approach provides comprehensive and reproducible proteomic data, supporting biomedical research, drug developmen......

    • • Advances in Antibody Characterization Techniques

      Antibody characterization techniques encompass a comprehensive set of modern bioanalytical methods designed to systematically analyze and characterize the sequence, structure, modifications, affinity, and functional properties of antibody molecules. As essential components of the immune system, antibodies play an irreplaceable role in fundamental research, disease diagnostics, and the development of therapeutic antibody drugs. With the rapid advancement of biopharmaceutical technologies, innovative th......

    • • Antibody Characterization Methods

      Antibody Characterization Methods encompass a range of experimental and analytical techniques designed to determine the specificity, affinity, and functional properties of antibodies. These methods are essential not only for basic scientific research but also for disease diagnostics, therapeutic antibody development, and biomarker discovery. Antibodies exert their biological effects through specific interactions with target antigens; however, rigorously validating antibody specificity and functionalit......

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