Raffinose Analysis Service
Raffinose is a trisaccharide composed of galactose, glucose, and fructose, belonging to the raffinose family of oligosaccharides (RFOs). It is widely found in leguminous plants, cereals, and root or tuber crops. In plants, raffinose participates in energy storage, carbon metabolism, and stress regulation, with its content closely related to plant maturation, stress responses, and germination processes. Raffinose analysis is of great significance for elucidating plant physiological mechanisms and improving crop quality, and it has been widely applied in plant metabolism research, nutritional component detection, fermentation process optimization, and the development of functional oligosaccharide products.
Moreno, J. et al. Enological Chemistry, 2012.
Figure 1. The Composition of Raffinose.
Services at MtoZ Biolabs
Based on advanced instruments and analytical platforms, MtoZ Biolabs has launched the raffinose analysis service which provides comprehensive detection and analysis of raffinose. Through precise chromatographic separation and highly sensitive mass spectrometric detection, this service accurately determines the content, purity, and structural characteristics of raffinose, and, combined with metabolomic analysis, evaluates its dynamic changes and stability under different biological systems or process conditions. The resulting data can be used for sample quality control, metabolic regulation studies, and process optimization, providing reliable support for both scientific research and industrial applications. MtoZ Biolabs offers services including but not limited to the following:
1. Quantitative Analysis
Using high-performance liquid chromatography (HPLC) or liquid chromatography–mass spectrometry (LC-MS/MS) techniques, the content and purity of raffinose in samples are accurately determined, ensuring data accuracy and reproducibility.
2. Structural Characterization
High-resolution mass spectrometry and nuclear magnetic resonance (NMR) are employed to analyze the molecular structure and configurational characteristics of raffinose, revealing its composition and structural stability.
3. Metabolomic Analysis
By integrating metabolomic strategies, the metabolic distribution and dynamic changes of raffinose under different biological systems or experimental conditions are analyzed to provide a basis for metabolic pathway research.
4. Stability Evaluation
By detecting changes in raffinose content and structure under different temperatures, pH values, or storage conditions, its physicochemical stability and application suitability are assessed.
Sample Submission Suggestions
1. Sample Types
Various types of samples are accepted, including plant tissues, seed extracts, microbial culture media, serum, plasma, and soil extracts. Both liquid, powder, and lyophilized forms are supported.
Note: Samples should be free of high salt, protein, or organic solvent residues to avoid interference with analytical results.
2. Sample Storage
Samples should be sealed and protected from light. For long-term storage, samples are recommended to be kept at -80°C; for short-term analysis, they may be stored at -20°C. Repeated freeze-thaw cycles should be avoided.
3. Sample Transportation
Liquid samples are recommended to be transported via dry ice cold chain. Lyophilized samples can be shipped at ambient temperature for short durations but must be protected from moisture, high temperatures, and light to ensure the stability of raffinose components and the reliability of analytical results.
Service Advantages
1. High-Sensitivity Detection Platform
Relying on advanced analytical platforms, high-resolution separation and precise quantification of raffinose can be achieved, ensuring reliable results.
2. Multidimensional Data Analysis
Comprehensive data support is provided, covering content determination, structural characterization, and metabolic profiling, to facilitate an in-depth understanding of the biological roles of raffinose.
3. Strict Quality Control System
Standard reference materials and quality control samples are used throughout the entire process to ensure data authenticity and reproducibility.
4. One-Stop Technical Service
A complete service workflow is offered, from sample analysis to data interpretation and report delivery, providing clients with efficient and professional support.
Applications
1. Plant Physiology Research
The raffinose analysis service can be applied to study the role of raffinose in plant stress resistance, seed germination, and carbon storage, revealing its regulatory mechanisms in plant adaptation to environmental changes.
2. Microbial Metabolism Research
By detecting the raffinose content in fermentation systems or culture media, this service enables analysis of its dynamic changes in microbial energy metabolism and carbon source utilization.
3. Metabolic Engineering and Biosynthesis Research
The raffinose analysis service helps evaluate the effects of metabolic pathway modifications on raffinose synthesis and degradation, guiding the optimization of metabolic regulation strategies.
4. Storage and Processing Monitoring
By detecting changes in raffinose content within samples, the stability and transformation characteristics of samples under different storage conditions or processing procedures can be assessed.
FAQ
Q1: Will the Presence of Other Oligosaccharides in the Sample Affect the Detection?
A1: No. High-resolution separation by HPLC or LC-MS/MS can effectively distinguish raffinose from structurally similar sugars such as stachyose and galactose.
Q2: Is Raffinose Prone to Degradation during Sample Storage?
A2: Partial degradation may occur under high-temperature or high-humidity conditions. It is recommended to store samples at low temperature and protected from light to ensure data accuracy.
Q3: Do Different Sample Matrices Affect the Detection Results?
A3: To some extent, yes. Interfering substances present in different matrices such as serum, plant tissues, and fermentation broth require corresponding pretreatment procedures to remove impurities.
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