Proteins O-glycan Profiling Service
Proteins O-glycan Profiling Service refers to a specialized analytical service that identifies, characterizes, and quantifies O-glycosylation modifications on the surface of proteins through a series of biochemical and analytical chemistry techniques. This service focuses on O-glycan modifications attached to serine (Ser) or threonine (Thr) residues of proteins, primarily analyzing glycan composition, branching patterns, glycosylation sites, and relative abundance.
He M. et al. Sig Transduct Target Ther. 2024.
O-glycosylation is a form of post-translational modification in which glycan chains are covalently attached to the hydroxyl groups of serine (Ser) or threonine (Thr) residues via O-glycosidic bonds. This modification is widely found in secreted and membrane proteins. O-glycans are highly diverse and structurally complex, lack a consensus sequence, and are synthesized through highly dynamic biosynthetic pathways, making them inherently unpredictable. Recent studies have revealed that O-glycosylation plays essential roles in regulating cellular processes such as proliferation, differentiation, immune recognition, adhesion, and signal transduction, and is closely associated with various diseases including cancer, diabetes, and autoimmune disorders. Therefore, comprehensive characterization of protein O-glycan composition, structure, and distribution is of great significance for fundamental biological research, disease mechanism studies, and the development of biotherapeutics.
Leveraging advanced chromatography, mass spectrometry, and bioinformatics platforms, MtoZ Biolabs provides Proteins O-glycan Profiling Service to systematically analyze O-glycan species, composition, structure, linkage patterns, and relative abundance. Our service supports both targeted protein analysis and profiling of complex biological samples, empowering biomedical research and biopharmaceutical development.
Analysis Workflow
Kang T. et al. Cell Rep Methods. 2024.
The main workflow of Proteins O-glycan Profiling Service is as follows:
1. Sample Pretreatment
Remove interfering substances such as salts and surfactants from the sample to concentrate the target proteins.
2. O-Glycan Release
Specifically release O-linked glycans using reductive β-elimination under alkaline conditions.
3. Glycan Enrichment and Purification
Purify and enrich the released glycans using techniques such as HILIC or solid-phase extraction.
4. Glycan Derivatization (Optional)
Perform fluorescent labeling if required by the project to enhance detection signals.
5. Mass Spectrometry Analysis
Analyze the glycans using MALDI-TOF MS, ESI-MS, or LC-MS/MS for structural elucidation.
6. Data Analysis
Integrate mass spectrometry data to identify glycan composition, linkage patterns, and relative abundance, supporting the construction of glycosylation profiles.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Proteins O-glycan Profiling Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Proteins O-glycan Profiling Service data, providing clients with a comprehensive data report.
4. Multi-level structural analysis: It can realize the accurate analysis of monosaccharide composition, connection order, branching structure and site specificity of O-sugar chains.
5. Standardization of the whole process: From sample pretreatment, sugar chain release, enrichment and purification to derivatization labeling and MS analysis, a rigorous standard operating procedure (SOP) has been established to ensure high consistency of data between different batches and provide reusable data support.
Sample Submission Suggestions
Sample Type: We accept a variety of biological samples, including but not limited to purified proteins, cell lysates, tissue samples, serum/plasma, saliva/urine and other body fluids.
Storage and Transportation: -80°C frozen storage, dry ice transportation, avoid repeated freezing and thawing.
Additional Notes: If the sample contains components that may interfere with mass spectrometry analysis (glycerol, detergents, salts, etc.), please indicate so that we can perform appropriate pretreatment. If you are unsure about sample preparation, MtoZ Biolabs can provide pretreatment services or detailed guidance, please contact our technical support team.
Applications
Application examples of Proteins O-glycan Profiling Service:
Disease Mechanism Research
Identify aberrant O-glycan patterns associated with cancers, diabetes, and neurodegenerative diseases to support mechanistic studies.
Biopharmaceutical Development and Quality Control
Evaluate the impact of glycosylation on drug efficacy, bioavailability, and immunogenicity.
Vaccine and Antibody Engineering
Analyze glycoprotein structures in vaccines to guide optimization of antibody glycosylation sites.
Cellular Communication Studies
Investigate the role of O-glycans in cell recognition, adhesion, and signal transduction to elucidate molecular interaction mechanisms.
Glycoengineering and Synthetic Biology
Provide structural insights for glycan design in synthetic glycoprotein engineering and support the construction of artificial glycosylation pathways.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
O-GlcNAcylation Profiling Service
N-glycosylation profiling service
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