Protein Palmitoylation Analysis

Protein palmitoylation refers to the modification process of adding one or more palmitic acids (C16 fatty acids) to the amino acid residues of proteins. It usually occurs on cysteine residues to form a covalent bond. Protein palmitoylation plays an important role in cell signal transduction, membrane localization, and protein-protein interactions.

MtoZ Biolabs provides a comprehensive Protein Palmitoylation Analysis Service designed to help researchers accurately detect, enrich, and characterize S-palmitoylated proteins across a wide range of complex biological samples. This service is suitable for recombinant proteins, cell lysates, tissue samples, and proteomics-level materials, supporting both targeted palmitoylation analysis of specific proteins and large-scale palmitoylation proteomics studies.

Our platform addresses major technical challenges such as low-abundance modification detection, thioester instability, and high background interference, ensuring stable and reliable analytical results. Through precise localization and quantitative assessment of palmitoylation events, MtoZ Biolabs provides strong support for studies on signal transduction, membrane trafficking, immune regulation, and disease mechanisms, advancing both fundamental research and translational applications.

Services at MtoZ Biolabs

1. Target Protein Palmitoylation Analysis

MtoZ Biolabs provides target protein palmitoylation analysis dedicated to the precise identification and characterization of palmitoylation modifications on specific proteins. By integrating the Acyl-Biotin Exchange (ABE) method or click chemistry labeling with high-resolution LC–MS/MS detection, we accurately identify palmitoylated cysteine residues and quantitatively evaluate modification levels. T

his service supports the investigation of how palmitoylation affects protein localization, membrane association, and signaling regulation, making it ideal for targeted studies of protein function and mechanism.

2. Palmitoylation Proteomics Analysis

MtoZ Biolabs' palmitoylation proteomics service combines high-resolution mass spectrometry with biotin-switch-based or metabolic labeling enrichment strategies to perform proteome-wide profiling of palmitoylation modifications. This high-throughput workflow enables systematic identification and quantification of palmitoylated proteins and modification sites in diverse biological samples.

Coupled with bioinformatics analysis, this service uncovers the dynamic regulatory roles of palmitoylation in signaling pathways, disease mechanisms, and protein trafficking, providing comprehensive data support for biomarker discovery and functional proteomics research.

Analysis Workflow

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Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein palmitoylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein palmitoylation analysis data providing clients with acomprehensive data report.

4. Comprehensive Analysis: Both target protein palmitoylation analysis and palmitoylation proteomics can be conducted in MtoZ Biolabs.

Sample Submission Requirements

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1. If you are providing tissue samples, please ship them to us on dry ice.

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.