Acylation Analysis Service
- Ultra-High Sensitivity: Detect low-abundance acylated peptides (≥0.1 fmol).
- Comprehensive Coverage: Multi-acyl type analysis in one pipeline.
- Speed & Precision: 2-week turnaround with >95% PTM identification accuracy.
- Expert Support: Customized experimental design and data interpretation by PTM specialists.
Protein acylation is a post-translational modification (PTM) involving the covalent attachment of acyl groups (e.g., acetyl, propionyl, butyryl) to lysine residues, regulating diverse cellular processes. It plays critical roles in enzyme activity, protein stability, subcellular localization, and interactions with DNA or other proteins. MtoZ Biolabs offers advanced acylation analysis service to decode site-specific acylation patterns, leveraging cutting-edge mass spectrometry and bioinformatics. Our platform empowers researchers in uncovering novel biomarkers, understanding disease mechanisms, and identifying therapeutic targets linked to dysregulated acylation.
Shang, S. et al. Signal Transduct Target Ther. 2022.
Figure 1. The Types of Acylation
Acylation dynamically regulates cellular metabolism, gene expression, and stress responses. Common types include: acetylation, palmitoylation, malonylation, succinylation, glutarylation, propionylation, crotonylation, and β-hydroxybutyrylation. Dysregulated acylation contributes to cancer, neurodegenerative disorders, cardiovascular diseases, and infections. For example, hyperacetylation of histones/nonhistone proteins drives oncogenic signaling, while defective palmitoylation disrupts neuronal synaptic function. Targeting acyltransferases (e.g., p300/CBP), deacylases (e.g., sirtuins, HDACs), or acylation "reader" proteins offers therapeutic potential. Small-molecule inhibitors (e.g., HDACi in cancer) and metabolites (e.g., NAD⁺ boosters for sirtuin activation) are under clinical development.
Jiang, N. et al. Biomed Pharmacother. 2023.
Figure 2. Acetylation in Pathogenesis
Services at MtoZ Biolabs
MtoZ Biolabs provides comprehensive acylation analysis service, including:
✔️ Global acylation site identification (LC-MS/MS)
✔️ Quantitative acylation dynamics (TMT/iTRAQ labeling)
✔️ Functional validation (mutagenesis + activity assays)
✔️ Customized panels for specific acyl types (acetyl, succinyl, malonyl, etc.)
✔️ Bioinformatics analysis (pathway mapping, disease correlation)
Sample compatibility: cells, tissues, serum, exosomes, and other complex biological samples.
Analysis Workflow
1. Sample Preparation: Protein extraction, tryptic digestion, acyl-peptide enrichment (immunoprecipitation/antibody-based).
2. LC-MS/MS Analysis: High-resolution Orbitrap platforms for deep coverage.
3. Data Processing: MaxQuant/Percolator for PTM identification.
4. Bioinformatics: Metascape/KEGG for pathway/network analysis.
5. Report: Detailed summary with validation-ready data.
Figure 3. The Workflow of Acetylation Analysis
Service Advantages
Applications
1. Basic Research: Elucidating acylation’s role in signaling/metabolism.
Jiang, N. et al. Biomed Pharmacother. 2023.
Figure 4. Protein Acetylation Regulates Cellular Metabolism Involved in Cardiac Function
2. Biomarker Discovery: Identifying acyl-based markers in deseases.
3. Drug Development: Validating targets for metabolic/neurological therapies.
4. Precision Medicine: Patient stratification via acylation profiles.
How Can We Help You?
1. Communication of Requirements: Fill out the inquiry form below to receive a customized solution.
2. Sample Submission: We support dry ice shipping and accept samples such as cells (1×107), body fluid (>200 μL), and tissue (>50 mg). For specific sample quantity requirements, please contact our technical staff.
3. Experiment and Analysis: Tailored analysis will be completed within 15 business days for routine projects, with real-time progress updates provided for complex projects.
4. Report Delivery: Reports include acylation site identification results, acylation modification quantitative data, mass spectrometry raw data and analysis files, acylated peptide sequence spectra (MS/MS spectra), bioinformatics analysis results .
Acylation is a linchpin of cellular regulation and disease. MtoZ Biolabs combines cutting-edge technology with deep PTM expertise to deliver acylation analysis service, accelerating your research from mechanism to therapy. Partner with us to decode the acylation code and unlock novel therapeutic opportunities.
How to order?
