Propionylation Proteomics Analysis Service
- Serum/Plasma: 100 µL
- Urine: 2 mL
- Animal Tissue: 200 mg
- Plant Tissue: 10 g
- Microorganisms: 500 µL
- Protein Extract: 5 mg
- Cells: 1×10⁸ cells
Propionylation is a significant post-translational modification (PTM) in which a propionyl group (such as propionyl-CoA) is attached to lysine residues in proteins through acyltransferase enzymes. This modification plays a crucial role in various biological processes, including metabolic regulation, enzyme activity modulation, cellular stress response, and epigenetic control. Recent studies have shown that propionylation is essential not only in bacterial metabolic networks but also in mammalian photosynthesis and stress response mechanisms. Thus, quantitative analysis of propionylated proteins is vital for understanding their biological functions and mechanisms.
MtoZ Biolabs offers Propionylation Proteomics Analysis Service that combines high-resolution LC-MS/MS with advanced antibody enrichment techniques, enabling precise identification and quantification of lysine propionylation sites in proteins. We are dedicated to delivering high-quality, customized propionylation analysis solutions to support researchers across diverse scientific fields.
Services at MtoZ Biolabs
MtoZ Biolabs provides Propionylation Proteomics Analysis Service that enabes large-scale identification and quantification of lysine propionylation sites across the entire proteome. This global approach provides comprehensive insights into the biological roles of propionylation in metabolic regulation, gene expression, and signal transduction pathways.
In addition to broad-spectrum proteomic profiling, we also offer targeted propionylation analysis which focuses on the precise modification status of selected proteins. This customized analysis is ideal for investigating specific targets involved in cancer, metabolic disorders, and neurodegenerative diseases. By tailoring our analytical strategies to individual research goals, MtoZ Biolabs delivers flexible and insightful solutions to accelerate discovery.
Analysis Workflow
Our Propionylation Proteomics Analysis Service follows a standardized workflow that ensures reliable and reproducible results:
1. Sample Preparation
Proteins are extracted from cells, tissues, or body fluids using a lysis buffer containing protease and deacetylase inhibitors to preserve propionylation modifications.
2. Protein Digestion
Proteins are digested into peptides using trypsin, preparing them for mass spectrometry analysis.
3. Peptide Enrichment
Anti-propionylation antibodies are used to selectively enrich propionylated peptides, minimizing interference from unmodified peptides and ensuring accurate detection of modification sites.
4. Mass Spectrometry Analysis
Enriched propionylated peptides are analyzed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), identifying and quantifying modification sites.
5. Data Analysis
Mass spectrometry data are analyzed using software such as MaxQuant and Proteome Discoverer, locating propionylation modification sites and quantifying the modification levels.
Figure 1. Propionylation Proteomics Analysis Workflow
Why Choose MtoZ Biolabs?
✅ Advanced Mass Spectrometry Platforms: We utilize high-resolution platforms like Thermo Fisher's Q Exactive HF and Orbitrap Fusion Lumos, ensuring precise detection and quantification of propionylation modifications.
✅ Comprehensive Analytical Capability: We offer both targeted propionylation analysis and global proteomics analysis, meeting diverse research needs.
✅ Flexible Quantification Methods: With TMT/iTRAQ labeling and Label-Free approaches, we offer multi-sample quantitative analysis, enhancing experimental efficiency and ensuring data reliability.
✅ Customized Experimental Plans: We customize experimental workflows to help you understand propionylation’s role in specific biological contexts.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
To ensure effective and accurate Propionylation Proteomics Analysis, MtoZ Biolabs requires the following:
1. Sample Type and Amount
2. Sample Shipping
Lyophilized samples can be shipped at room temperature. Liquid samples should be shipped on ice or with dry ice. Fresh tissue samples must be shipped with dry ice or refrigerated, avoiding prolonged exposure to room temperature.
If your sample is special or in limited quantity, please contact us in advance to confirm feasibility. MtoZ Biolabs also provides detailed sample submission guidelines, available upon consultation with our technical support.
Applications
Propionylation Proteomics Analysis Service is widely applied in the following areas:
· Epigenetic Regulation: Propionylation modifications impact chromatin structure and gene expression, and analyzing propionylation sites helps reveal its role in epigenetic control.
· Metabolic Signaling: Propionylation plays a key role in regulating metabolic enzymes. Studying its involvement in metabolism aids in disease treatment.
· Disease Mechanism Research: Propionylation is linked to cancer and neurodegenerative diseases. Analyzing propionylation in disease-related proteins helps reveal its role in disease mechanisms.
· Drug Target Discovery: As a dynamic PTM, propionylation serves as a reference for drug target development, especially in studying drug responses and resistance mechanisms.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
If you are interested in exploring the biological functions of propionylation modifications or need a customized analysis plan, please contact MtoZ Biolabs. We provide precise, professional Propionylation Proteomics Analysis Service to support your research and product development. We also offer other PTM proteomics services, including phosphorylation, glycosylation, acetylation, and ubiquitination, helping you comprehensively study protein function regulation from multiple dimensions.
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