Polysaccharide Absolute Configuration Determination Service
- Raw analytical data from instrument platforms;
- Processed result tables (Excel or CSV format);
- Comprehensive analysis reports (PDF format) containing experimental methods, data interpretation, and main conclusions;
- Spectral and chromatographic image files (TIFF or PNG format) for comparison and visualization.
MtoZ Biolabs employs a multi-platform analytical system to provide the polysaccharide absolute configuration determination service which enables precise identification of the absolute configuration of polysaccharides and their monosaccharide components. This service is widely applied in natural product structural analysis, polysaccharide synthesis research, and functional polysaccharide development, offering reliable data support for carbohydrate chemistry studies and biomaterial design.
Overview
Polysaccharide absolute configuration refers to the stereochemical orientation of monosaccharides within polysaccharides, including D- or L-configuration and the α/β anomeric form of glycosidic bonds. This characteristic directly determines the three-dimensional structure, physicochemical properties, and biological activity of polysaccharides. Polysaccharides with different configurations show significant differences in solubility, viscosity, antioxidant properties, immune regulation, and receptor recognition. Therefore, accurately analyzing the absolute configuration of polysaccharides is a key step in elucidating the relationship between their structure and function.
Zhang, N. et al. CHM, 2018.
Figure 1. Structures of D-Glucose and L-Glucose.
Polysaccharide Absolute Configuration Determination Service at MtoZ Biolabs
1. Absolute Configuration Determination Based on Gas Chromatography-Mass Spectrometry (GC-MS)
By derivatizing monosaccharides and analyzing their retention times and mass spectral fragmentation patterns, this method accurately distinguishes D/L configurations and glycosidic bond types, making it suitable for various polysaccharide systems.
2. Absolute Configuration Determination Based on Liquid Chromatography-Mass Spectrometry (LC-MS)
Combining high-resolution separation with mass spectrometric detection, this approach enables highly sensitive analysis of monosaccharide derivatives and is ideal for complex or highly polar samples.
3. Absolute Configuration Determination Based on Circular Dichroism (CD)
By utilizing differences in optical activity between molecular configurations, this method allows rapid qualitative assessment of the chiral characteristics of polysaccharides.
4. Absolute Configuration Determination Based on Nuclear Magnetic Resonance (NMR) Spectroscopy
Through the analysis of chemical shifts and coupling constants, this technique directly determines the conformation and linkage patterns of monosaccharide residues within polysaccharides, providing reliable and intuitive results.
Why Choose MtoZ Biolabs?
✅ Multi-Platform Integration: Combines GC-MS, LC-MS, CD, and NMR technologies to ensure accurate and reliable results.
✅ High Sensitivity Detection: Capable of analyzing trace samples and distinguishing complex configuration types.
✅ Multi-Dimensional Analysis: Simultaneously identifies the D/L configuration and α/β anomeric form of monosaccharides.
✅ Strict Quality Control: Implements standardized procedures throughout the process to guarantee data consistency and reproducibility.
✅ Professional Technical Support: Provides expert data interpretation and result consultation by an experienced analytical team.
Applications of Polysaccharide Absolute Configuration Determination Service
1. Pharmaceutical and Biopharmaceutical Research
Used for configuration analysis of polysaccharide-based drugs, glycoconjugates, and vaccine carriers to ensure structural accuracy and correlation with biological activity.
2. Biochemical and Metabolic Mechanism Studies
Reveals stereochemical characteristics of polysaccharides during biosynthesis, degradation, and metabolic pathways, supporting functional mechanism exploration.
3. Biomaterials and Engineering Research
Evaluates configuration composition and conformational stability of polysaccharide-based materials, providing a foundation for material modification and performance optimization.
4. Synthesis and Modification Verification
Confirms the stereochemical configuration of synthetically produced or chemically modified polysaccharides to ensure product consistency and structural controllability.
5. Quality Control and Standardization Analysis
Provides reliable analytical support for batch consistency, purity assessment, and structural standardization of polysaccharide products.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Polysaccharide Configuration Analysis Results
4. Spectral, Mass Spectrometric, and Chromatographic Data
5. Data Interpretation and Result Report
6. Raw Data Files
FAQ
Q1: What types of samples are suitable?
A1: The polysaccharide absolute configuration determination service provided by MtoZ Biolabs is applicable to various sample types, including natural polysaccharides, chemically synthesized polysaccharides, polysaccharide derivatives, fermentation liquids, and plant or microbial extracts. To ensure accurate results, samples should be of high purity and free from proteins, salts, or other interfering substances.
Q2: What is the service general workflow?
A2:
Q3: What data formats are provided?
A3: MtoZ Biolabs delivers standardized, multi-format data outputs suitable for scientific analysis and result presentation. Typical deliverables include:
If special analytical requirements exist, data formats can be customized according to project specifications.
Q4: How should I prepare my samples?
A4: To ensure accuracy and reproducibility, MtoZ Biolabs recommends following these guidelines before sample submission:
(1) Sample Form: Solid powder or solution forms are acceptable;
(2) Purity Requirements: Avoid high salt, surfactant, or organic solvent residues;
(3) Storage Conditions: Store at -20℃for short-term or -80℃ for long-term preservation;
(4) Transportation: Transport with dry ice using sealed, leak-proof containers;
(5) Additional Information: Indicate sample origin, processing method, and analysis objectives to allow optimal testing design.
For more Information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.
Start Your Project with MtoZ Biolabs
Contact us to discuss your experimental design or request a quote. Whether you aim to elucidate the configuration of polysaccharides or investigate their structure-function relationships in biological systems, MtoZ Biolabs provides precise, reliable analytical support and professional technical services.
How to order?
