Peroxisome Metabolomics Analysis Services
Peroxisomes are single-membrane-bound organelles found in eukaryotic cells, enriched with various metabolic enzymes that are deeply involved in lipid metabolism, including the β-oxidation of long-chain fatty acids and the biosynthesis of ether phospholipids and cholesterol. Beyond lipid processing, peroxisomes also play a vital detoxification role by degrading reactive oxygen species such as hydrogen peroxide, thereby maintaining intracellular redox homeostasis. Dysfunction in peroxisomal metabolism can lead to lipid dysregulation, oxidative stress imbalance, and is associated with numerous inherited metabolic disorders.
MtoZ Biolabs offers comprehensive Peroxisome Metabolomics Analysis Services by leveraging high-throughput mass spectrometry platforms and deep expertise in metabolomics, enabling researchers to explore peroxisomal metabolic functions, biogenesis, and their roles in disease at a systems-wide level.
Services at MtoZ Biolabs
MtoZ Biolabs offers systematic Peroxisome Metabolomics Analysis Services encompassing both targeted and untargeted metabolomics strategies to support qualitative and quantitative analysis of peroxisome-related metabolites across various sample types. Our platform combines optimized sample preparation workflows, advanced chromatographic and mass spectrometry systems, and expert bioinformatics interpretation—adaptable to various sample types and research goals.
· Untargeted Metabolomics Analysis
Using high-resolution LC-MS and GC-MS platforms, we provide a comprehensive metabolic profile of peroxisome-associated pathways. This approach is ideal for capturing broad-spectrum metabolic changes in response to stress, genetic variation, or disease. Multivariate statistical modeling and pathway enrichment analysis allow for the discovery of novel functional metabolites and underlying regulatory networks.
· Targeted Metabolomics Analysis
For hypothesis-driven studies, we offer highly sensitive and specific quantification of key metabolites involved in known pathways. Leveraging Multiple Reaction Monitoring (MRM) or Parallel Reaction Monitoring (PRM), we quantify biomarkers related to lipid catabolism, hydrogen peroxide detoxification, and acetyl-CoA utilization, delivering robust support for mechanistic studies and target validation.
Analysis Workflow
Our Peroxisome Metabolomics Analysis Services follow a rigorous and standardized workflow to ensure data reliability, analytical reproducibility, and biological relevance:
1. Sample Preparation and Extraction
Sample-specific protocols are applied for homogenization, centrifugation, protein precipitation, and metabolite extraction—ensuring consistency across tissues, cells, or biofluids.
2. Chromatographic Separation
LC or GC techniques are selected based on compound polarity and volatility, with tailored mobile phase gradients to maximize resolution.
3. Mass Spectrometry Acquisition
High-resolution instruments (e.g., QTOF, Orbitrap) are employed for either full-scan data collection or targeted MRM/PRM monitoring, depending on analytical objectives.
4. Data Processing and Quantification
Raw data undergo peak picking, retention alignment, normalization, and metabolite identification. Statistically significant metabolic alterations are then extracted.
5. Functional Annotation and Pathway Analysis
Key differential metabolites are mapped to curated pathways (KEGG, HMDB), and statistical modeling reveals their biological implications.
Why Choose MtoZ Biolabs?
· Comprehensive Coverage: Supports both discovery-driven and hypothesis-based analysis strategies, covering lipids, organic acids, alcohols, ketones, amino acids, and their derivatives.
· Flexible Analytical Platforms: Compatible with LC-MS, GC-MS, and NMR technologies to accommodate diverse compound classes and experimental designs.
· Custom Method Development: Species-specific and pathway-specific workflows can be developed to address complex biological questions and specialized targets.
· Rigorous Quality Assurance: Standardized protocols and multi-layer QC checkpoints ensure reproducible and high-fidelity results.
· One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
FAQ
Q1: Can I submit whole tissue or whole-cell samples without pre-isolating peroxisomes?
Yes. MtoZ Biolabs accepts native tissue or cell lysate samples. Our team can selectively isolate peroxisome-enriched fractions using differential centrifugation, density gradient separation, or immunoaffinity-based enrichment prior to metabolite extraction. This ensures the resulting metabolomic profile accurately reflects peroxisome-specific metabolic activity, supporting both basic research and mechanistic investigations.
Q2: Do you support integrated multi-omics analysis across metabolomics, transcriptomics, and proteomics?
Absolutely. MtoZ Biolabs offers integrated multi-omics analytics to explore regulatory connections across transcriptional, translational, and metabolic layers. Our pipeline includes correlation analysis, co-expression networks, and pathway-based enrichment to support biomarker discovery and holistic understanding of complex biological systems.
What Could be Included in the Report?
✔ High-resolution raw MS data (RAW files) and peak intensity tables
✔ Annotated metabolite lists with database references
✔ Statistical reports with differential metabolite profiles (e.g., volcano plots, heatmaps)
✔ Pathway enrichment analysis reports (KEGG/HMDB)
✔ Network visualizations and functional annotation of metabolic pathways
✔ High-purity peroxisome samples (if isolation service is requested)
MtoZ Biolabs is committed to advancing subcellular multi-omics research. By combining organelle-level separation techniques with high-resolution mass spectrometry, our Peroxisome Metabolomics Analysis Services provide comprehensive insights into peroxisomal function in health and disease. Contact us for customized study design and project consultation.
Related Services
Peroxisome Proteomics Services
Peroxisome Isolation and Purification Service
How to order?
