Peroxisome Proteomics Services
MtoZ Biolabs delivers expert Peroxisome Proteomics Services that enable researchers to comprehensively profile peroxisomal protein composition, dynamic expression patterns, and post-translational modifications. As essential subcellular organelles, peroxisomes orchestrate fatty acid β-oxidation, reactive oxygen species detoxification, and metabolic regulation. Our precision proteomics approach generates the high-quality data needed to unravel mechanisms of metabolic reprogramming, oxidative stress responses, and disease pathogenesis.
We employ a combination of density gradient centrifugation and immuno-enrichment to isolate peroxisomes with exceptional purity. These are then analyzed on state-of-the-art mass spectrometry platforms, including Orbitrap Fusion Lumos, complemented by an advanced multidimensional data analysis pipeline. This end-to-end solution provides tailored proteomic insights for your research objectives.
Services at MtoZ Biolabs
MtoZ Biolabs offers a fully integrated Peroxisome Proteomics Services workflow, designed to support every stage of your study—from experimental design and sample prep to quantitative analysis and bioinformatics interpretation:
· Comprehensive Peroxisomal Protein Identification
Utilizing high-resolution LC-MS/MS, we achieve deep proteome coverage of membrane proteins, matrix enzymes, metabolic factors, and PEX family components—facilitating functional categorization and pathway mapping.
· Quantitative Proteomics (Label-free / TMT / DIA)
We apply diverse quantification strategies to detect condition-specific expression changes, supporting robust comparative proteomics and differential analysis designs.
· Post-translational Modification Profiling (Phosphorylation, Acetylation, Ubiquitination)
Enrichment and targeted methods (PRM/SWATH) capture low-abundance regulatory PTMs, enabling detailed signaling pathway investigations.
Analysis Workflow
MtoZ Biolabs follows a rigorous, standardized protocol to guarantee sample integrity, analytical sensitivity, and data reliability:
1. Sample Processing & Peroxisome Isolation
Differential and density-gradient centrifugation, coupled with peroxisome-marker validation, ensures highly purified organelle fractions.
2. Protein Extraction & Enzymatic Digestion
Custom lysis buffers and a two-step (Lys-C + Trypsin) digestion workflow ensure efficient extraction and peptide recovery for membrane-rich proteomes.
3. Mass Spectrometry Analysis
Leveraging Orbitrap platforms, we maximize detection of both high- and low-abundance proteins.
4. Data Processing & Functional Annotation
We integrate UniProt, GO, PeroxiBase, and other databases for protein classification, subcellular localization prediction, enrichment analysis, and protein-protein interaction network modeling.
Why Choose MtoZ Biolabs?
✅ Advanced Analysis Platform: We blend the capabilities of Orbitrap Fusion Lumos, Q Exactive to deliver high-resolution, high-dynamic-range proteomic data.
✅ Purity-First Isolation: Dual centrifugation and immuno-bead enrichment minimize cytosolic contamination and boost peroxisomal specificity.
✅ Flexible Quantitative Strategy: Supports TMT/iTRAQ, Label-Free, SWATH modalities to match diverse experimental schemes.
✅ Complete PTM Coverage: From phosphorylation to ubiquitination, we design workflows for comprehensive PTM profiling.
✅ High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all peroxisome proteomics data, providing clients with a comprehensive data report.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
Our Peroxisome Proteomics Services are applicable to basic life science research, disease mechanism exploration, and crop improvement. Specific applications include:
· Metabolic Disease Research: Cataloging enzymes involved in β-oxidation and ROS clearance, aiding insight into conditions like obesity and fatty liver disease.
· Plant Stress Biology: Deciphering peroxisome response networks under drought, salinity, and cold—fueling innovations in stress-resistant crop breeding.
· Toxicology & Environmental Health: Profiling proteomic alterations due to toxins (e.g., pharmaceuticals, heavy metals), advancing understanding of antioxidant defense mechanisms.
· Organelle Crosstalk Studies: Mapping interactions between peroxisomes, mitochondria, and ER via membrane-protein interactomics.
· PTM-Regulatory Network Construction: Identifying modification sites to reconstruct signaling pathways and dynamic regulatory landscapes.
FAQ
Q1: How is peroxisome purity and integrity maintained to prevent cytosolic contamination?
We combine differential and density-gradient centrifugation, with optional immunomagnetic bead enrichment customized per sample type. Purity is confirmed via Western blot for peroxisomal markers (PMP70, Catalase) alongside cytosolic (GAPDH) and mitochondrial (COX IV) controls—ensuring trustworthy downstream proteomics.
Q2: How do you optimize extraction and digestion of hydrophobic peroxisomal membrane proteins?
We use MS-compatible detergents, high-salt and mild-alkaline lysis, sonication, heat treatment, followed by two-step enzymatic digestion (Lys-C + Trypsin) and SP2-bead desalting. This strategy enhances peptide yield and mass spectrometry compatibility, enabling robust membrane proteome analysis.
As a full-service multi-omics provider, MtoZ Biolabs also offers isolation and proteomics for mitochondria, ER, ribosomes, lysosomes, and more—enabling comprehensive studies of subcellular structures and metabolic regulation. Contact us to discuss tailored solutions and project assessments based on our Peroxisome Proteomics Services.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
How to order?
