Peptide Molecular Weight Distribution Analysis Service
Peptide Molecular Weight Distribution Analysis Service is a high-performance liquid chromatography (HPLC)-based quality evaluation service designed to precisely characterize the molecular weight distribution of peptide mixtures. This service is applicable to a wide range of fields, including peptide drug development, assessment of natural peptide extracts, and analysis of functional peptide components in food products.
Peptides, as essential signaling molecules and functional components within biological systems, play critical roles across drug development, functional foods, and tissue engineering. Given that peptide samples are often complex mixtures, key properties such as bioactivity, stability, and transdermal permeability are significantly influenced by their molecular weight distribution. Therefore, accurately assessing molecular weight profiles is essential for ensuring product consistency, purity control, and structural characterization.
Xie Y. et al. Int J Mol Sci. 2012.
Using HPLC, peptide mixtures are separated within a chromatographic column based on differences in molecular weight, polarity, and hydrophobicity. As individual peptides elute at different retention times, the detector captures signals to generate a chromatogram, where each peak corresponds to peptide fractions of specific molecular weights. This enables effective analysis of molecular weight distribution in complex peptide samples.
Leveraging an advanced HPLC platform, MtoZ Biolabs offers Peptide Molecular Weight Distribution Analysis Service to support quantitative characterization of raw peptide materials, synthetic peptide blends, or natural peptide mixtures. This service enables clients to gain deeper insight into sample composition and functional potential.
Analysis Workflow
The general workflow of Peptide Molecular Weight Distribution Analysis Service is as follows:
1. Sample Preparation
Dissolve peptide samples and remove interfering impurities to ensure analytical accuracy.
2. Standard Curve Establishment
Prepare a calibration curve using peptide standards of known molecular weights.
3. HPLC Separation
Separate peptide components using an appropriate chromatographic column and mobile phase system.
4. Signal Detection
Acquire chromatographic signals via a sensitive detector.
5. Data Interpretation
Analyze peak areas and retention times to generate a molecular weight distribution profile.
6. Report Delivery
Provide a detailed report including distribution plots, peak area statistics, average molecular weight, and other key metrics.
Service Advantages
1. Accurate Distribution Profiling: Enables high-confidence molecular weight range calculation based on a calibrated standard curve.
2. High-Throughput Capability: Supports rapid analysis of large sample batches to enhance R&D efficiency.
3. Robust and Reproducible Methodology: Delivers high reproducibility and consistency, suitable for reliable quantitative comparison.
Sample Submission Suggestions
Sample Type: Supports purified peptides, synthetic peptide mixtures, or naturally extracted peptides.
Transportation Requirements: Lyophilized samples can be transported at room temperature; solution samples are recommended to be transported with refrigeration or on dry ice to avoid repeated freeze–thaw cycles.
Additional Notes: If the sample is special or in small quantity, please contact us in advance to confirm feasibility. MtoZ Biolabs will provide you with the most suitable analysis plan and technical support.
Applications
Application examples of Peptide Molecular Weight Distribution Analysis Service:
Drug Development and Quality Control
Evaluate the purity, stability, and molecular consistency of peptide-based drugs.
Functional Peptide Analysis in Food
Identify the molecular weight distribution of peptides added to food, ensuring functionality and compliance.
Synthesis Process Optimization
Compare the molecular weight differences of peptides under different synthesis conditions.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Molecular Weight Standard Curve and Fitting Parameters
4. Molecular Weight Distribution Results Table
5. Data Analysis Explanation Document
6. Raw Data Files
Related Services
How to order?
