Peptides Analysis Service

Peptides are composed of two or more amino acids linked together by peptide bonds formed through dehydration condensation. Peptides consisting of fewer than ten amino acids are referred to as oligopeptides (small peptides), while those with more than ten are classified as polypeptides. If a peptide chain exceeds fifty amino acids, it is considered a protein. Peptides play extensive physiological roles in biological systems, including participation in cell signal transduction, regulation of physiological processes, and functioning as hormones. Therefore, peptide analysis has become critically important. Currently, peptides analysis service has multiple methods, such as mass spectrometry (MS), Edman degradation, and high-performance liquid chromatography (HPLC). The choice of analytical method depends on the specific properties of the peptide segment being analyzed.

 



Figure 1. 3D Structure of Peptides.

 

Services at MtoZ Biolabs

Leveraging a comprehensive peptide analysis platform, MtoZ Biolabs provides a full spectrum of peptides analysis service including but not limited to amino acid composition analysis, purity assessment, molecular weight determination (LC-MS), molecular weight distribution, peptide identification, peptide sequencing, post-translational modification analysis, circular dichroism spectroscopy, and infrared spectroscopy. Our expert laboratory and analytical team ensures strict control over every step, from sample preparation to data analysis, guaranteeing high efficiency in detection processes and accurate results.

 

1. Amino Acid Composition Analysis

Amino acid composition analysis is one of the fundamental aspects of peptide chemistry research. It provides preliminary insight into the chemical structure characteristics of peptides and the distribution of specific functional amino acids, offering valuable scientific information for peptide structure and function studies. MtoZ Biolabs offers two methods for amino acid composition analysis: high-performance liquid chromatography (HPLC) and automatic amino acid analyzer technology. Both methods effectively determine the amino acid composition of peptide sequences.

 

2. Purity Assessment

Peptide purity analysis is crucial for biopharmaceutical research and bioproduct manufacturing, as it directly impacts product quality and safety. It is an essential step in the research and production process. Our HPLC-based method enables precise assessment of peptide purity, providing reliable support for your research and production needs.

 



Figure 2. Application Areas for Peptides of Different Purities.

 

3. Molecular Weight (LC-MS) Analysis

Molecular weight is one of the fundamental properties of peptides. By accurately determining the molecular weight, it is possible to assist in identifying peptide composition and structure, differentiate between different peptides, and achieve precise peptide characterization. MtoZ Biolabs offers peptide molecular weight analysis using various methods, including LC-MS, MALDI-TOF, SEC-MALS, SEC, and SDS-PAGE.

 

4. Molecular Weight Distribution Analysis

Molecular weight distribution analysis provides precise measurement of the molecular weight range and relative content of components within a peptide mixture. This analysis is critical for understanding the complexity and purity of peptide samples, as well as for controlling synthesis or degradation processes. We primarily employ the SEC method to assess peptide molecular weight distribution.

 

5. Peptide Identification

Peptide identification is essentially the process of assigning an "identity tag" to a peptide, determining its amino acid sequence and corresponding source protein. At MtoZ Biolabs, peptide identification is conducted primarily using mass spectrometry, where acquired data is matched against theoretical protein sequences to determine peptide composition.

 



Figure 3. Peptide Identification Process.

 

6. Peptide Sequencing

Peptide sequencing enables the precise determination of amino acid sequences in peptides without relying on any pre-existing sequence data. This is a key step in understanding the structure-function relationship of peptides. MtoZ Biolabs provides peptide sequencing services using both mass spectrometry and Edman degradation techniques to determine peptide amino acid sequences.

 

7. Circular Dichroism (CD) Spectroscopy Analysis

Circular dichroism (CD) spectroscopy is primarily used to investigate the secondary structure of peptides, such as α-helices, β-sheets, and random coils, as well as their relative content and distribution. We collect CD spectra of samples in the far-ultraviolet range (190–260 nm) and analyze their secondary structures using CDNN software.

 

8. Infrared Spectroscopy (IR) Analysis

Infrared (IR) spectroscopy analyzes peptide molecular vibrations upon infrared light exposure to obtain structural information. MtoZ Biolabs utilizes advanced infrared spectroscopy instruments to directly generate IR spectra of peptides.

 

Service Advantages

1. Advanced Analytical Platform

MtoZ Biolabs has a comprehensive experimental analysis platform, established a one-stop peptides analysis service platform for you to solve all the peptide analysis.

 

2. Experienced Technical Team

Our experienced technical team provides one-on-one consultation, addressing all technical inquiries with precision.

 

3. Transparent Pricing

We offer transparent pricing with no hidden or additional fees, along with lifetime post-service support.

 

Sample Submission Suggestions

1. Sample Types

(1) Single or mixed peptide samples.

 

(2) Liquid/solid samples, cells, bacteria, tissues, blood, urine, paraffin-embedded tissue sections, immunopeptides, etc.

Note: If you have any special requirements or need assistance with sample preparation, please contact us.

 

Applications

1. Biopharmaceutical Development

The peptides analysis service is widely used in the development of biological drugs to identify the interaction between target proteins and its candidate drugs, and evaluate the selectivity and effectiveness of the drug. This helps accelerate the discovery and optimization process of biopharmaceuticals.

 

2. Disease Diagnosis and Biomarker Identification

By analyzing peptide fragments of proteins, we can identify and quantify disease-related protein expression levels and modifications, aiding in early disease diagnosis and biomarker discovery.

 

3. Drug Quality Control

Peptides analysis service can be used to compare with reference peptide maps to evaluate and monitor protein quality during the production process, ensuring drug consistency and quality stability of the production.

 

Case Study

Identifying actionable tumor antigens is essential for developing multiple cancer immunotherapies, including T cell receptor-engineered T cells and patient-specific mRNA or peptide vaccines. Most known tumor antigens are identified through extensive molecular characterization and are considered canonical if derived from the protein-coding regions of the genome. Tumor-associated peptides can be eluted from tumors, bound to human leukocyte antigens, and identified via mass spectrometry by matching spectra to reference databases. Recently, mass spectrometry-based immunopeptidomics has enabled the discovery of non-canonical antigens originating from non-coding regions or produced through unconventional antigen-processing mechanisms. By integrating transcriptomics and ribosome profiling, this method can identify thousands of non-canonical peptides, many of which may be tumor-exclusive. While matching spectra against vast non-canonical reference databases can generate false positives, advancements in high-sensitivity mass spectrometry, analytical validation, and sophisticated bioinformatics solutions hold promise for fully characterizing presented antigens and clinically relevant targets.

 

Deliverables

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Data Analysis, Result Tables, and Project Reports

 

4. Raw Data Files

 

MtoZ Biolabs has achieved outstanding results in peptide analysis and has successfully partnered with numerous biopharmaceutical companies, providing a professional peptides analysis service platform. Upholding a "customer-first" philosophy, we are committed to delivering the highest quality services to each client. We prioritize effective communication, gaining in-depth insights into customer needs and research goals before project initiation, and providing professional guidance and tailored solutions. Throughout the project, we ensure timely updates on progress and address any inquiries. Upon project completion, we deliver detailed and accurate analytical reports, offering professional interpretations to help clients better understand and utilize the results. If you require similar testing services, feel free to contact us for consultation!