GPC Analysis Service

Gel Permeation Chromatography (GPC), also known as size-exclusion chromatography, is a widely adopted technique for molecular weight analysis of polymers. Unlike methods that rely on chemical composition, GPC separates molecules based on their hydrodynamic volume, making it particularly suitable for analyzing molecular weight and distribution in polydisperse systems such as synthetic polymers, biopolymers, and natural products.

 

As a professional third-party analytical service provider, MtoZ Biolabs offers efficient and precise GPC Analysis Service built on an advanced chromatographic platform. Whether supporting fundamental research during early-stage product development or performing quality control during manufacturing, we deliver standardized, industry-compliant data that empowers our clients to make informed decisions and accelerate innovation.

 

Technical Principle  

GPC operates on the principle of molecular size-based separation. As samples pass through a column packed with porous materials, larger molecules are excluded from the pores and elute first, while smaller molecules enter the pores and are retained longer. This results in effective separation by size — or more precisely, by molecular weight.

 



Wang, Z. et al. Adv Phys Res. 2024.

Figure 1. Principle of Gel Permeation Chromatography  

 

GPC can be coupled with a variety of detectors — including refractive index (RI), ultraviolet (UV), light scattering (LS), and viscometric (VIS) detectors — to accommodate diverse analytical needs. Compared to other molecular weight analysis methods, GPC offers the following advantages:  

1. Non-destructive and mild operation conditions  

2. Broad applicability to synthetic polymers, natural macromolecules, and biopolymers  

3. Insight into molecular weight distribution, not just average values  

4. High reproducibility and standardized methodology  

 

Analysis Workflow  

At MtoZ Biolabs, our GPC Analysis Service follows strict standard operating procedures (SOPs) to ensure analytical accuracy and reproducibility. The workflow includes:

1. Sample Preparation and Pretreatment  

Based on sample type and solubility information, appropriate solvents (e.g., THF, water, buffered acids) are selected. Samples are dissolved, filtered, and purified to eliminate particulates and ensure compatibility with the system.

 

2. System Configuration and Method Setup  

Chromatographic columns, mobile phases, and detectors are selected according to the analytical goal and sample characteristics. Flow rate, column temperature, and other parameters are optimized for stable performance.

 

3. Calibration with Standards  

Calibration curves are established using certified standards (e.g., polystyrene, polyethylene glycol) with known molecular weights. For absolute molecular weight analysis, light scattering or viscometric detectors can be employed, eliminating the need for calibration standards.

 

4. Sample Analysis and Data Acquisition  

Prepared samples are injected into the system for separation and detection. Retention times, peak areas, and detector responses are recorded in real time for each fraction.

 

5. Data Processing and Report Generation  

Dedicated software is used to calculate Mn, Mw, PDI, and other key metrics. Reports include molecular weight distribution graphs, chromatographic conditions, sample identifiers, and interpretive results.

 

Service Advantages  

MtoZ Biolabs provides comprehensive and reliable GPC analysis service with the following strengths:

1. Professional Platform with Advanced Instrumentation  

Equipped with high-performance GPC systems and multiple detector options, we support a wide range of molecular weight ranges and sample types — from oligomers to ultrahigh-molecular-weight polymers.

 

2. Flexible Detection Modes  

We offer standard GPC (RI/UV), multi-detector GPC (RI + LS + VIS), and SEC-MALS setups to meet both routine quantification and advanced structural characterization needs.

 

3. Rigorous Quality Control  

All processes adhere to validated SOPs, with concurrent calibration and system checks to guarantee data traceability, reliability, and integrity.

 

4. Support for Complex Samples and Custom Analysis  

For samples with poor solubility, structural complexity, or low stability, we offer customized solvent systems and pretreatment strategies to ensure accurate and reproducible results.

 

5. Rapid Turnaround and Expert Support  

Our dedicated technical and customer service teams provide timely assistance, experimental design consultation, and post-analysis interpretation, helping clients translate data into actionable insights.

 

Applications  

GPC analysis plays a critical role across multiple research and industrial domains. Key applications include:

1. Drug Delivery and Controlled Release Materials  

In the development of nanocarriers such as PLGA microspheres, PEGylated systems, and liposomes, GPC determines how molecular weight affects encapsulation efficiency and release kinetics.

 

2. Purity and Stability Assessment of Biopolymers  

Used for evaluating molecular weight and distribution in polysaccharides, biogels, and biodegradable materials, GPC helps determine product consistency and functionality.

 

3. Food and Natural Product Development  

Applied to natural polysaccharides, plant-derived gums, and functional proteins, GPC supports structural analysis and quality assessment, aiding formulation design and regulatory compliance.

 

Case Study

1. Separation and Characterization of Gelatins Using Aqueous Gel Permeation Chromatography with Advanced Detection Systems

 



Crawshaw, B. et al. American Chemical Society. 2018.

Figure 2. GPC Chromatograms of Five Different Gelatin Materials

 

2. Investigation of Sample Preparation for High Temperature Gel Permeation Chromatography Using a Low Solvent Consumption Method

 



Boborodea, A. et al. Polym Test. 2017.

Figure 3. Evolution of the Mw Based on the DRI Chromatograms

 

FAQ

Q1: What types of samples are suitable for GPC?  

GPC is applicable to most natural or synthetic polymers, biopolymers, modified materials, and food polysaccharides. Samples should be soluble and chemically stable in the selected solvent.

 

Q3: Can absolute molecular weight be measured?  

Yes. Absolute molecular weight can be determined using light scattering (LS) or multi-angle light scattering (MALS) detectors, both of which are available at MtoZ Biolabs.

 

Q4: What is included in the report?  

Standard reports provide molecular weight parameters (Mn, Mw, PDI), chromatograms, analytical conditions, and reference standards. Custom formats or additional annotations can be included upon request.

 

Q6: Do you offer method development or long-term monitoring services?  

Yes. We support method development, validation, and ongoing QC monitoring for enterprise clients and research projects. Batch analysis services are also available.

 

As a cornerstone technique for polymer characterization, Gel Permeation Chromatography (GPC) is extensively applied in both academic and industrial settings. Leveraging advanced instrumentation, deep technical expertise, and a standardized management system, MtoZ Biolabs delivers accurate, reliable GPC Analysis Service tailored to your R&D and quality assurance needs. If you are seeking a robust solution for molecular weight analysis, MtoZ Biolabs is here to provide professional support and end-to-end service.