Multiple Reaction Monitoring Mass Spectrometry Analysis Service

Multiple Reaction Monitoring Mass Spectrometry is a targeted quantitative analysis technique based on triple quadrupole mass spectrometry, known for its high sensitivity and specificity. It is widely applied in proteomics, metabolomics, and small molecule research. The core principle involves selectively monitoring parent and fragment ion pairs (transitions) to achieve precise quantification.

 



Boja, E. S. et al. Korean J Lab Med. 2011.

Figure1. A Schematic of a Triple Quadrupole Mass Spectrometer (Qqq-Ms) Commonly Used In MRM-MS Analysis

Traditional quantitative methods, such as ELISA and Western blotting, have limitations in terms of specificity and throughput. While non-targeted mass spectrometry techniques like DIA or PRM offer broad coverage, they may not meet the high-precision requirements for specific target molecules. Multiple Reaction Monitoring Mass Spectrometry addresses this gap, providing an ideal solution. Its stability, reproducibility, and high-throughput capabilities make it indispensable in biomarker validation, drug metabolism studies, and molecular mechanism exploration.

 

Service at MtoZ Biolabs

As a leader in mass spectrometry technology, MtoZ Biolabs offers Multiple Reaction Monitoring Mass Spectrometry Service supported by world-class mass spectrometry platforms (such as the AB Sciex QTRAP 6500+ and Thermo TSQ Altis) and an expert technical team. We provide a full-service workflow, from method development to data analysis, to support your research projects with the highest standards:

1.Experimental Design

The expert team at MtoZ Biolabs will develop a customized experimental plan based on the client’s research objectives. Through in-depth communication, we precisely identify the target molecules, ensuring the scientific and feasible nature of the research plan.

 

2.Sample Preparation and Processing

High-quality samples are essential for obtaining reliable data. We offer optimized sample preparation processes, including protein/peptide enrichment, target molecule extraction, and stability enhancement measures, ensuring that sample quality meets mass spectrometry detection standards.

 

3.Method Development and Optimization

MRM method development is a key step in technical implementation. Based on the chemical properties of the target molecules, we develop and optimize MRM methods for parent and fragment ion pairs, ensuring the best balance of sensitivity, linear range, and specificity.

 

4.Data Acquisition and Quality Control

Using triple quadrupole mass spectrometers, we acquire high-quality MRM data and apply strict quality control procedures to verify the stability and reliability of the data.

 

5.Data Analysis and Interpretation

We utilize professional analysis software (such as Skyline and MultiQuant) to provide detailed quantitative results and in-depth data interpretation reports, helping clients quickly gain key research insights.

 



Figure2. Analysis workflow of Multiple Reaction Monitoring Mass Spectrometry Service

 

Service Advantages

1.High Sensitivity and Specificity

Multiple Reaction Monitoring Mass Spectrometry significantly reduces background noise by accurately selecting parent and fragment ion pairs, enabling the reliable detection of low-abundance target molecules with a detection limit down to the picomolar level.

 

2.Simultaneous Analysis of Multiple Target Molecules

Multiple Reaction Monitoring Mass Spectrometry allows for the analysis of dozens to hundreds of target molecules in a single experiment, greatly improving research efficiency.

 

3.High Reproducibility and Stability

With its exceptional reproducibility, Multiple Reaction Monitoring Mass Spectrometry has become a gold standard in both scientific research and clinical testing.

 

4.Flexible Customized Services

We adjust the number of target molecules, detection range, and sensitivity requirements based on the client’s needs, providing truly personalized solutions that meet specific research objectives.

 

Applications

1.Disease Biomarker Validation

Multiple Reaction Monitoring Mass Spectrometry can accurately quantify the levels of candidate biomarkers, providing reliable data for early disease diagnosis, efficacy evaluation, and prognosis prediction.

 

2.Drug Metabolism and Pharmacodynamics Research

Evaluate the pharmacokinetics and target interactions of drugs in vivo, providing crucial insights for new drug development and personalized medicine.

 

3.Post-Translational Modifications (PTM) Analysis

Multiple Reaction Monitoring Mass Spectrometry can quantitatively analyze post-translational modifications such as phosphorylation, methylation, and acetylation, offering in-depth insights into functional protein research.

 

4.Functional Protein Studies

Quantifying the expression levels of key functional proteins to uncover the regulatory mechanisms of biological processes.

 

5.Industrial Quality Control and Food Safety

Multiple Reaction Monitoring Mass Spectrometry is widely used in industrial applications, such as detecting food additives, contaminants, and ensuring compliance with safety standards.

 

Sample Submission Suggestions

The Multiple Reaction Monitoring Mass Spectrometry Service is highly compatible with a wide range of sample types. MtoZ Biolabs accepts the following types of samples:

• Biological Fluids: Serum, plasma, urine, saliva, etc.
• Tissue Samples: Fresh or frozen biological tissue homogenates.
• Cell Samples: Cell lysates, culture supernatants.
• Synthetic Samples: Purified proteins, peptides, standard solutions.

*Recommended Sample Amount:

For each sample, it is recommended to provide ≥50 µg of protein or peptide fragments. For low-abundance molecule analysis, samples with higher concentrations can be provided.

*Storage and Shipping:

Samples should be stored at ultra-low temperatures (-80°C) and shipped with dry ice to ensure stability.

 

In targeted quantitative analysis, Multiple Reaction Monitoring Mass Spectrometry Service has become an indispensable technological tool due to its outstanding performance. MtoZ Biolabs, with its cutting-edge technology platforms and service philosophy, is committed to providing clients with precise and reliable solutions. Whether for basic research or applied development, we aim to be your trusted partner. Choose MtoZ Biolabs to take your research to the next level!