mRNA Modification LC-MS Analysis Service
mRNA Modification LC-MS Analysis Service is a highly sensitive modified nucleoside analysis service based on liquid chromatography-mass spectrometry (LC-MS), which aims to accurately identify and quantify different types of chemical modifications in mRNA. It is particularly suitable for drawing modification spectra, comparing modification differences between different treatment groups, or verifying the change trend of specific modified nucleosides.
Shi H. et al. Mol Cancer. 2020.
In eukaryotes, messenger RNA (mRNA) is not only a carrier of genetic information but also undergoes various covalent modifications that regulate its processing, including splicing, transport, stability, translation efficiency, and degradation. Common mRNA modifications include N6-methyladenosine (m6A), 5-methylcytosine (m5C), pseudouridine (Ψ), and N1-methyladenosine (m1A). These modifications are collectively known as "epitranscriptomic modifications" and play critical roles in processes such as embryonic development, stem cell differentiation, tumor formation, and antiviral responses.
Systematic analysis of mRNA modifications, especially quantitative detection and modification profiling, is crucial for unveiling gene expression regulation networks, mechanisms of cell fate determination, and disease development mechanisms. LC-MS, as a high-sensitivity and high-resolution analytical platform, has become a core tool for studying RNA modifications.
Based on advanced chromatography and mass spectrometry platforms, MtoZ Biolabs offers mRNA Modification LC-MS Analysis Service providing high-sensitivity identification and accurate quantification of various mRNA chemical modifications. This service can help researchers comprehensively analyze mRNA modification profiles, revealing their functional mechanisms in gene expression regulation, cell fate determination, and disease progression.
Analysis Workflow
Zhang Y. et al. Exp Mol Med. 2022.
The main analysis workflow for mRNA Modification LC-MS Analysis Service is as follows:
1. Sample Preparation
Extract high-quality total RNA, then isolate and purify the mRNA.
2. Enzymatic Hydrolysis
Use an optimized enzymatic digestion system to degrade mRNA into nucleosides.
3. Chromatographic Separation
Employ optimized chromatographic protocols to separate the target modified nucleosides.
4. Mass Spectrometry Detection
Utilize a high-resolution mass spectrometry platform to collect both precursor and fragment ion data.
5. Data Analysis and Report Generation
Identify modification types, quantify modification abundance, and generate modification profiles and visual reports.
Service Advantages
1. Comprehensive Coverage of Multiple RNA Modification Types: Supports m6A, m5C, m1A and other common modifications.
2. High Sensitivity and High Resolution: Can detect low-abundance modifications and distinguish heterogeneous modification forms.
3. Advanced Analysis Platform: MtoZ Biolabs established an advanced mRNA Modification LC-MS Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
5. Flexible Customized Service: Supports targeted detection and method development for specific modification types.
Sample Submission Suggestions
MtoZ Biolabs offers RNA extraction services and accepts both total RNA and purified mRNA. Please ensure that samples are transported on dry ice to avoid freeze-thaw cycles.
Feel free to contact MtoZ Biolabs' technical team for tailored sample submission guidance before submitting your samples.
Applications
The applications of mRNA Modification LC-MS Analysis Service include, but are not limited to:
Epitranscriptomics Research
Mapping mRNA modification profiles specific to cells or tissues.
Disease Mechanism Research
Unveiling the patterns of mRNA modifications in diseases and their biological significance.
Stem Cells and Regenerative Medicine
Studying the regulatory role of modifications in stem cell fate determination and tissue development.
Drug Development
Assessing the impact of drugs on mRNA modifications, aiding in the elucidation of pharmacological mechanisms.
Virus Infections and Immune Evasion Mechanism Exploration
Investigating how pathogens manipulate host mRNA modifications to regulate the infection process.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Mass Spectrometry-based m6A Modification Quantitative Analysis Service
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